Functional Characterization of Three Aquaglyceroporins from Trypanosoma brucei in Osmoregulation and Glycerol Transport

2011 ◽  
Vol 27 (3-4) ◽  
pp. 411-420 ◽  
Author(s):  
Björn Bassarak ◽  
Nestor L. Uzcátegui ◽  
Caroline Schönfeld ◽  
Michael Duszenko
Keyword(s):  
Trypanosoma Brucei ◽  
Functional Characterization ◽  
Glycerol Transport

scholarly journals Functional characterization of two paralogs that are novel RNA binding proteins influencing mitochondrial transcripts of Trypanosoma brucei

RNA ◽  
2012 ◽  
Vol 18 (10) ◽  
pp. 1846-1861 ◽  
Author(s):  
L. Kafkova ◽  
M. L. Ammerman ◽  
D. Faktorova ◽  
J. C. Fisk ◽  
S. L. Zimmer ◽  
...  
Keyword(s):  
Trypanosoma Brucei ◽  
Binding Proteins ◽  
Rna Binding ◽  
Rna Binding Proteins ◽  
Functional Characterization ◽  
Mitochondrial Transcripts

scholarly journals Functional characterization of two novel parvulins in Trypanosoma brucei

FEBS Letters ◽  
2010 ◽  
Vol 584 (13) ◽  
pp. 2901-2908 ◽  
Author(s):  
Jian Yuan Goh ◽  
Cheng-Yu Lai ◽  
Luuan Chin Tan ◽  
Daiwen Yang ◽  
Cynthia Y. He ◽  
...  
Keyword(s):  
Trypanosoma Brucei ◽  
Functional Characterization

scholarly journals Proximity Interactions among Basal Body Components in Trypanosoma brucei Identify Novel Regulators of Basal Body Biogenesis and Inheritance

mBio ◽  
2017 ◽  
Vol 8 (1) ◽  
Author(s):  
Hung Quang Dang ◽  
Qing Zhou ◽  
Veronica W. Rowlett ◽  
Huiqing Hu ◽  
Kyu Joon Lee ◽  
...  
Keyword(s):  
Trypanosoma Brucei ◽  
Basal Body ◽  
Functional Characterization ◽  
Body Protein ◽  
Superresolution Microscopy ◽  
Novel Proteins ◽  
Organelle Segregation ◽  
Body Components ◽  
Flagellar Axoneme

ABSTRACT The basal body shares similar architecture with centrioles in animals and is involved in nucleating flagellar axonemal microtubules in flagellated eukaryotes. The early-branching Trypanosoma brucei possesses a motile flagellum nucleated from the basal body that consists of a mature basal body and an adjacent pro-basal body. Little is known about the basal body proteome and its roles in basal body biogenesis and flagellar axoneme assembly in T. brucei . Here, we report the identification of 14 conserved centriole/basal body protein homologs and 25 trypanosome-specific basal body proteins. These proteins localize to distinct subdomains of the basal body, and several of them form a ring-like structure surrounding the basal body barrel. Functional characterization of representative basal body proteins revealed distinct roles in basal body duplication/separation and flagellar axoneme assembly. Overall, this work identified novel proteins required for basal body duplication and separation and uncovered new functions of conserved basal body proteins in basal body duplication and separation, highlighting an unusual mechanism of basal body biogenesis and inheritance in this early divergent eukaryote. IMPORTANCE The basal body in the early-branching protozoan Trypanosoma brucei nucleates flagellum assembly and also regulates organelle segregation, cell morphogenesis, and cell division. However, the molecular composition and the assembly process of the basal body remain poorly understood. Here, we identify 14 conserved basal body proteins and 25 trypanosome-specific basal body proteins via bioinformatics, localization-based screening, and proximity-dependent biotin identification. We further localized these proteins to distinct subdomains of the basal body by using fluorescence microscopy and superresolution microscopy, discovered novel regulators of basal body duplication and separation, and uncovered new functions of conserved basal body proteins in basal body duplication and separation. This work lays the foundation for dissecting the mechanisms underlying basal body biogenesis and inheritance in T. brucei .

FUNCTIONAL CHARACTERIZATION OF THE SERINE/THREONINE PROTEIN PHOSPHATASE 5 FROM TRYPANOSOMA BRUCEI

2006 ◽  
Vol 92 (6) ◽  
pp. 1152-1161 ◽  
Author(s):  
Sedrick Anderson ◽  
Candace Jones ◽  
Lipi Saha ◽  
Minu Chaudhuri
Keyword(s):  
Trypanosoma Brucei ◽  
Protein Phosphatase ◽  
Functional Characterization ◽  
Protein Phosphatase 5

scholarly journals Molecular and Functional Characterization of Grapevine NIPs through Heterologous Expression in aqy-Null Saccharomyces cerevisiae

2020 ◽  
Vol 21 (2) ◽  
pp. 663 ◽  
Author(s):  
Farzana Sabir ◽  
Sara Gomes ◽  
Maria C. Loureiro-Dias ◽  
Graça Soveral ◽  
Catarina Prista
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Functional Characterization ◽  
High Water ◽  
Specific Amino Acid ◽  
Transmembrane Flux ◽  
Glycerol Transport ◽  
Internal Ph ◽  
Cytosolic Acidification ◽  
Glycerol Permeability

Plant Nodulin 26-like Intrinsic Proteins (NIPs) are multifunctional membrane channels of the Major Intrinsic Protein (MIP) family. Unlike other homologs, they have low intrinsic water permeability. NIPs possess diverse substrate selectivity, ranging from water to glycerol and to other small solutes, depending on the group-specific amino acid composition at aromatic/Arg (ar/R) constriction. We cloned three NIPs (NIP1;1, NIP5;1, and NIP6;1) from grapevine (cv. Touriga Nacional). Their expression in the membrane of aqy-null Saccharomyces cerevisiae enabled their functional characterization for water and glycerol transport through stopped-flow spectroscopy. VvTnNIP1;1 demonstrated high water as well as glycerol permeability, whereas VvTnNIP6;1 was impermeable to water but presented high glycerol permeability. Their transport activities were declined by cytosolic acidification, implying that internal-pH can regulate NIPs gating. Furthermore, an extension of C-terminal in VvTnNIP6;1M homolog, led to improved channel activity, suggesting that NIPs gating is putatively regulated by C-terminal. Yeast growth assays in the presence of diverse substrates suggest that the transmembrane flux of metalloids (As, B, and Se) and the heavy metal (Cd) are facilitated through grapevine NIPs. This is the first molecular and functional characterization of grapevine NIPs, providing crucial insights into understanding their role for uptake and translocation of small solutes, and extrusion of toxic compounds in grapevine.

Functional Characterization of Glyoxalase II from Trypanosoma brucei

2006 ◽  
Vol 2006 (Spring) ◽  
Author(s):  
Alexandra Wendler ◽  
Thorsten Irsch ◽  
R. Luise Krauth-Siegel
Keyword(s):  
Trypanosoma Brucei ◽  
Functional Characterization ◽  
Glyoxalase Ii
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