scholarly journals Gene Expression Profiling of Adipose Tissues in Obesity Susceptible and Resistant Rats under a High Fat Diet

2011 ◽  
Vol 27 (3-4) ◽  
pp. 327-340 ◽  
Author(s):  
Jeong In Joo ◽  
Jong Won Yun
Keyword(s):  
Gene Expression ◽  
Gene Expression Profiling ◽  
Expression Profiling ◽  
High Fat Diet ◽  
High Fat ◽  
Adipose Tissues

scholarly journals Targeted Multiplex Gene Expression Profiling to Measure High-Fat Diet and Metformin Effects on Fetal Gene Expression in a Mouse Model

2018 ◽  
Vol 26 (5) ◽  
pp. 683-689
Author(s):  
Neeta L. Vora ◽  
Matthew R. Grace ◽  
Lisa Smeester ◽  
Sarah K. Dotters-Katz ◽  
Rebecca C. Fry ◽  
...  
Keyword(s):  
Gene Expression ◽  
Mouse Model ◽  
Gene Expression Profiling ◽  
Expression Profiling ◽  
High Fat Diet ◽  
High Fat

Identification of genes expressed differentially in subcutaneous and visceral fat of cattle, pig, and mouse

2005 ◽  
Vol 21 (3) ◽  
pp. 343-350 ◽  
Author(s):  
Daisuke Hishikawa ◽  
Yeon-Hee Hong ◽  
Sang-gun Roh ◽  
Hisae Miyahara ◽  
Yukihiko Nishimura ◽  
...  
Keyword(s):  
Gene Expression ◽  
Adipose Tissue ◽  
High Fat Diet ◽  
Animal Species ◽  
Fat Deposition ◽  
High Fat ◽  
Adipose Tissues ◽  
Fat Accumulation ◽  
Fat Depots ◽  
Visceral Adipose

The factors that control fat deposition in adipose tissues are poorly understood. It is known that visceral adipose tissues display a range of biochemical properties that distinguish them from adipose tissues of subcutaneous origin. However, we have little information on gene expression, either in relation to fat deposition or on interspecies variation in fat deposition. The first step in this study was to identify genes expressed in fat depot of cattle using the differential display RT-PCR method. Among the transcripts identified as having differential expression in the two adipose tissues were cell division cycle 42 homolog (CDC42), prefoldin-5, decorin, phosphate carrier, 12S ribosomal RNA gene, and kelch repeat and BTB domain containing 2 (Kbtbd2). In subsequent experiments, we determined the expression levels of these latter genes in the pig and in mice fed either a control or high-fat diet to compare the regulation of fat accumulation in other animal species. The levels of CDC42 and decorin mRNA were found to be higher in visceral adipose tissue than in subcutaneous adipose tissue in cattle, pig, and mice. However, the other genes studied did not show consistent expression patterns between the two tissues in cattle, pigs, and mice. Interestingly, all genes were upregulated in subcutaneous and/or visceral adipose tissues of mice fed the high-fat diet compared with the control diet. The data presented here extend our understanding of gene expression in fat depots and provide further proof that the mechanisms of fat accumulation differ significantly between animal species.

Gene expression profiles reveal effect of a high-fat diet on the development of white and brown adipose tissues

Gene ◽  
2015 ◽  
Vol 565 (1) ◽  
pp. 15-21 ◽  
Author(s):  
Hyeng-Soo Kim ◽  
Zae Young Ryoo ◽  
Sang Un Choi ◽  
Sanggyu Lee
Keyword(s):  
Gene Expression ◽  
High Fat Diet ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
High Fat ◽  
Adipose Tissues ◽  
Brown Adipose

scholarly journals Gene expression profiling reveals distinct features of various porcine adipose tissues

2013 ◽  
Vol 12 (1) ◽  
pp. 75 ◽  
Author(s):  
Chaowei Zhou ◽  
Jie Zhang ◽  
Jideng Ma ◽  
Anan Jiang ◽  
Guoqing Tang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Expression Profiling ◽  
Expression Profiling ◽  
Adipose Tissues ◽  
Distinct Features

scholarly journals Integrated Multiomic Analysis Reveals the High-Fat Diet Induced Activation of the MAPK Signaling and Inflammation Associated Metabolic Cascades via Histone Modification in Adipose Tissues

2021 ◽  
Vol 12 ◽  
Author(s):  
Zhe Wang ◽  
Ming Zhu ◽  
Meng Wang ◽  
Yihui Gao ◽  
Cong Zhang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Adipose Tissue ◽  
Energy Metabolism ◽  
Histone Modification ◽  
High Fat Diet ◽  
Whole Genome ◽  
Rna Seq ◽  
High Fat ◽  
Adipose Tissues

BackgroundThe number of diet induced obese population is increasing every year, and the incidence of type 2 diabetes is also on the rise. Histone methylation and acetylation have been shown to be associated with lipogenesis and obesity by manipulating gene expression via the formation of repression or activation domains on chromosomes.ObjectiveIn this study, we aimed to explore gene activation or repression and related biological processes by histone modification across the whole genome on a high-fat diet (HFD) condition. We also aimed to elucidate the correlation of these genes that modulated by histone modification with energy metabolism and inflammation under both short-term and long-term HFD conditions.MethodWe performed ChIP-seq analysis of H3K9me2 and H3K9me3 in brown and white adipose tissues (WATs; subcutaneous adipose tissue) from mice fed with a standard chow diet (SCD) or HFD and a composite analysis of the histone modification of H3K9me2, H3K9me3, H3K4me1 and H3K27ac throughout the whole genome. We also employed and integrated two bulk RNA-seq and a single-nuclei RNA sequencing dataset and performed western blotting (WB) to confirm the gene expression levels in adipose tissue of the SCD and HFD groups.ResultsThe ChIP-seq and transcriptome analysis of mouse adipose tissues demonstrated that a series of genes were activated by the histone modification of H3K9me2, H3K9me3, H3K4me1, and H3K27ac in response to HFD condition. These genes were enriched in Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways involved in lipogenesis, energy metabolism and inflammation. Several genes in the activated mitogen-activated protein kinase (MAPK) pathway might be related to both inflammation and energy metabolism in mice, rats and humans fed with HFD for a short or long term, as showed by bulk RNA-seq and single nuclei RNA-seq datasets. Western blot analyses further confirmed the increased expression of MET, VEGFA and the enhanced phosphorylation ratio of p44/42 MAPK upon HFD treatment.ConclusionThis study expanded our understanding of the influence of eating behavior on obesity and could assist the identification of putative therapeutic targets for the prevention and treatment of metabolic disorders in the future.

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