Diagnosis of Cervical Thymoma by Fine Needle Aspiration Biopsy with Flow Cytometry

2002 ◽  
Vol 46 (6) ◽  
pp. 1129-1132 ◽  
Author(s):  
T. Brent Ponder ◽  
Brian T. Collins ◽  
Maj Christopher S. Bee ◽  
Alan B. Silverberg ◽  
Leonard E. Grosso ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Fine Needle Aspiration ◽  
Fine Needle Aspiration Biopsy ◽  
Needle Aspiration ◽  
Aspiration Biopsy ◽  
Fine Needle ◽  
Needle Aspiration Biopsy

scholarly journals Fine-needle aspiration biopsy and flow cytometry immunophenotyping of lymphoid and myeloproliferative disorders of the spleen

Cancer ◽  
2002 ◽  
Vol 99 (2) ◽  
pp. 118-127 ◽  
Author(s):  
Pio Zeppa ◽  
Marco Picardi ◽  
Gilda Marino ◽  
Giancarlo Troncone ◽  
Franco Fulciniti ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Fine Needle Aspiration ◽  
Fine Needle Aspiration Biopsy ◽  
Myeloproliferative Disorders ◽  
Needle Aspiration ◽  
Aspiration Biopsy ◽  
Fine Needle ◽  
Needle Aspiration Biopsy

Fine Needle Aspiration Biopsy with Flow Cytometry Is a Reliable Method for Defining Immunophenotype of Prognostic Value in T Lymphoblastic Lymphoma.

Blood ◽  
2009 ◽  
Vol 114 (22) ◽  
pp. 3928-3928
Author(s):  
Beata Ostrowska ◽  
Grzegorz Rymkiewicz ◽  
Joanna Romejko-Jarosinska ◽  
Katarzyna Blachnio ◽  
Katarzyna Domanska-Czyz ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Fine Needle Aspiration ◽  
Prognostic Value ◽  
Fine Needle Aspiration Biopsy ◽  
Needle Aspiration ◽  
Lymphoblastic Lymphoma ◽  
Aspiration Biopsy ◽  
Fine Needle ◽  
Needle Aspiration Biopsy ◽  
T Lymphoblastic Lymphoma

Abstract Abstract 3928 Poster Board III-864 Background and aim In contrast to T lymphoblastic leukemia (T-ALL), there is a little data on the incidence and prognostic value of immunologic subtypes of adult T lymphoblastic lymphoma (T-LBL) as the use of flow cytometry in the absence of leukemia may be problematic. Our aim was to define immunophenotype of T-LBL and T-ALL in 51 consecutive patients by use of the flow cytometry (FCM) of tissue aspirates if peripheral blood (PB) and bone marrow (BM) were uninvolved. We also evaluated prognostic value of immunophenotype and clinical features of adult patients treated on uniform ALL protocol. Methods Between 1997 and 2006, 51 adult patients with T-LBL/ALL were treated according to the GMALL (German Multicenter Study Group for Adult ALL) 05/93 and T-LBL/2004 protocols (D.Hoelzer et al., Blood 2002; 99:4379). Immunophenotype was determined by specimen immunohistochemical staining and by FCM of cellular suspension obtained from lymph nodes (n=22), skin tumors (n=2) or mediastinal mass (n=9) by fine needle aspiration biopsy (FNAB), as well as of BM (n=6), PB (n=7) and pleural fluid (n=5). Disease subtype was defined according to WHO 2008 classification. Pro-T: cCD3+, CD7+, CD2-, CD1a-, CD34+/-; pre-T: cCD3+, CD7+, CD2+, CD1a-, CD34+/-; cortical T: cCD3+, CD7+, CD2+, CD1a+, CD34-, CD4+, CD8+; medullary T: cCD3+, sCD3+, CD7+, CD2+, CD1a-, CD34-, CD4+ or CD8+. Recognition of pan-T-cell CD antigens (pTAg) expression included: CD1a, CD2, cCD3, sCD3, CD4, CD5, CD7, CD8 and CD10, CD16&CD56, CD56, CD13, CD15, CD33. Results Patients (pts) characteristics: ALL (BM+ >20%): 37%, LBL: 63%, age < 35: 80%, males: 75%, median WBC: 22 G/L, Hb: 13.6 g/dl, plt: 267 G/L, mediastinal mass (MM): 92%, primary CNS+: 10%, PS 0-1: 60%, LDH > normal: 69% of pts. Immunophenotype: pro-T: 20%, pre-T: 12%, cortical: 51%, medullary: 12%, blastic plasmacytoid dendritic cell neoplasm with skin manifestation: 6% of pts. Number of pTAg present: 0-3: 39%, and 4-7: 61% of pts. Most frequently expressed pTAg were: CD7: 86%, CD5: 78%, CD2: 69%, CD1a: 47%, and CD56: 59%. Myeloid markers: CD13/33/15 were expressed in 14%/23%/10% of pts. Complete and partial remission (mostly residual MM) rate was 75% and 21%. With a median follow up for surviving patients of 62 months, 5 yr overall (OS) and disease-free (DFS) survival (95%C.I.) was 45% (31%; 59%) and 46% (32%, 60%), respectively. 5 yr OS for pts with CD2 and more than 3 pTAg present was 61% and 63% compared to 7% and 19% for pts without CD2 and 3 or less pTAg, (p=0.004 and 0.025) respectively. CD1a expression was favorable but of unconfirmed significance (p=0.09). On Cox's analysis of clinical features, only PS and LDH were predictive for OS and DFS and only expression of CD2 among immunophenotypic variants was significant for OS (p=0.004) and DFS (p=0.004). Conclusion Combined use of fine needle aspiration biopsy and flow cytometry is a reliable method for defining immunologic subtype of lymphoblastic lymphoma. In a prospective evaluation of 51 consecutive T-LBL/ALL patients treated on GMALL protocols, expression of CD2 antigen – mostly consistent with cortical and pre-T subtypes, and presence of more than 3 pTAg along with PS less than 2 and normal LDH were predictive for favorable outcome. Disclosures: No relevant conflicts of interest to declare.

scholarly journals CXCR3, CCR5, and CRTH2 Chemokine Receptor Expression in Lymphocytes Infiltrating Thyroid Nodules with Coincident Hashimoto’s Thyroiditis Obtained by Fine Needle Aspiration Biopsy

2016 ◽  
Vol 2016 ◽  
pp. 1-7 ◽  
Author(s):  
Jan Jiskra ◽  
Marie Antošová ◽  
Jan Krátký ◽  
Hana Vítková ◽  
Zdeňka Límanová ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Fine Needle Aspiration ◽  
Peripheral Blood ◽  
Chemokine Receptors ◽  
Thyroid Nodules ◽  
Fine Needle Aspiration Biopsy ◽  
Needle Aspiration ◽  
Aspiration Biopsy ◽  
Fine Needle ◽  
Needle Aspiration Biopsy

Objective. To determine the expression of chemokine receptors in lymphocytes from thyroid nodules and peripheral blood in patients with and without Hashimoto’s thyroiditis (HT). Patients and Methods. The study included 46 women with thyroid nodules and HT and 60 women with thyroid nodules without HT (controls) who underwent a fine needle aspiration biopsy (FNAB). Expression of chemokine receptors CXCR3, CCR5, and CRTH2 was assessed by flow cytometry in lymphocytes from FNAB samples and from peripheral blood. Results. The percentage of CRTH2+ lymphocytes was higher in nodules with HT in comparison with controls, both in FNAB samples (13.95 versus 6.7%, p=0.008) and in peripheral blood (6.7 versus 5.13%, p=0.047), and positively correlated with serum antibodies to thyroid peroxidase (r=0.243; p=0.026) and negatively correlated with thyroid volume (r=-0.346; p=0.008). Lymphocytes from neoplastic nodules showed a higher expression of both CXCR3 and CCR5 than those from hyperplastic ones. Conclusion. Flow cytometry performed in FNAB samples may serve as a good tool in investigation of intrathyroidal expression of immunological parameters. In our study, the CRTH2 expression on thyroid-infiltrating lymphocytes as well as on lymphocytes from peripheral blood was increased in HT as compared to controls.

Fine needle aspiration biopsy and flow cytometry in the diagnosis of lymphoma

2007 ◽  
Vol 37 (1) ◽  
pp. 71-79 ◽  
Author(s):  
Gillaume J. Swart ◽  
Colleen Wright ◽  
Karen Brundyn ◽  
Erna Mansvelt ◽  
Marita du Plessis ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Fine Needle Aspiration ◽  
Fine Needle Aspiration Biopsy ◽  
Needle Aspiration ◽  
Aspiration Biopsy ◽  
Fine Needle ◽  
Needle Aspiration Biopsy
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