scholarly journals Differentially Expressed Protein Profile of Renal Tubule Cell Stimulated by Elevated Uric Acid Using SILAC Coupled to LC-MS

2011 ◽  
Vol 27 (1) ◽  
pp. 91-98 ◽  
Author(s):  
Hong Quan ◽  
Xue Peng ◽  
Shuwen Liu ◽  
Fu Bo ◽  
Lv Yang ◽  
...  
Keyword(s):  
Uric Acid ◽  
Renal Tubule ◽  
Protein Profile ◽  
Differentially Expressed ◽  
Tubule Cell ◽  
Differentially Expressed Protein ◽  
Renal Tubule Cell

Effect of basement membrane and colloid osmotic pressure on renal tubule cell volume

1977 ◽  
Vol 233 (4) ◽  
pp. F325-F332
Author(s):  
M. A. Linshaw ◽  
F. B. Stapleton ◽  
F. E. Cuppage ◽  
J. J. Grantham
Keyword(s):  
Basement Membrane ◽  
Osmotic Pressure ◽  
Cell Volume ◽  
Cell Size ◽  
Renal Tubule ◽  
Colloid Osmotic Pressure ◽  
Outer Diameter ◽  
Tubule Cell ◽  
Cation Pump ◽  
Renal Tubule Cell

Renal tubule cell volume is thought to be kept constant by a cation pump. When active transport is blocked, intracellular impermeant solutes cause cells to swell. Cell size is then determined by transmembrane hydrostatic and colloid osmotic forces. We studied the importance of passive transmembrane forces in determining cell size in isolated rabbit proximal straight tubules (PST). We blocked active solute transport with ouabain and evaluated subsequent changes in cell size by measuring outer diameter of nonperfused tubules. Tubules in a ouabain and 6 g/100 ml protein bath swelled only 40% above control. However, removal of the tubule basement membrane with collagenase dissipated a transmembrane hydrostatic pressure and caused more swelling. Final cell volume was determined largely by bath protein concentration. Tubules in ouabain and collagenase swelled enormously in hyponcotic protein, moderately in isoncotic protein, and could be shrunk below control in hyperoncotic protein. Intracellular colloid osmotic pressure was estimated to exceed 38 cmH20. We conclude that hydrostatic and colloid osmotic forces are major determinants of cell size in isolated PST treated with ouabain.

scholarly journals The Effect of Chronic Progressive Nephropathy on the Incidence of Renal Tubule Cell Neoplasms in Control Male F344 Rats

2002 ◽  
Vol 30 (6) ◽  
pp. 681-686 ◽  
Author(s):  
John C. Seely ◽  
Joseph K. Haseman ◽  
Abraham Nyska ◽  
Douglas C. Wolf ◽  
Jeffrey I. Everitt ◽  
...  
Keyword(s):  
Renal Tubule ◽  
Tubule Cell ◽  
Control Male ◽  
Renal Tubule Cell ◽  
F344 Rats ◽  
Male F344 Rats

Effect of metabolic inhibitors on renal tubule cell volume

1980 ◽  
Vol 239 (6) ◽  
pp. F571-F577 ◽  
Author(s):  
M. A. Linshaw
Keyword(s):  
Cell Volume ◽  
Cell Size ◽  
Renal Tubule ◽  
Cation Transport ◽  
Metabolic Inhibitors ◽  
Cell Swelling ◽  
Tubule Cell ◽  
Bathing Medium ◽  
Cation Pump ◽  
Renal Tubule Cell

Renal tubule cell volume is thought to be kept constant by a cation pump. Ouabain, by inhibiting Na+-K+-ATPase, blocks cation transport with resultant cell swelling, but the degree of swelling is less than expected were active cation transport completely inhibited. Although the relatively rigid tubule basement membrane may limit swelling of ouabain-treated tubules, some investigators have alternatively postulated that an energy-dependent ouabain-insensitive cation pump regulates cell size. This notion derives from studies of renal cortical slices in which metabolic inhibitors such as 2,4-dinitrophenol (DNP) cause more swelling than ouabain. We blocked cellular metabolism of isolated rabbit proximal straight tubules by adding metabolic inhibitors to or removing acetate and glucose (energy substrate) from the bathing medium and evaluated subsequent changes in cell size by measuring outer diameter of nonperfused tubules. In isotonic medium, cell volume increased 36% with addition of 10(-4) M ouabain, 40% with 10(-2) M DNP, 46% with 10(-3) M cyanide, 39% with ouabain + DNP + cyanide, and 37% with removal of bath substrate (P = NS). We conclude that renal tubule cell volume is not regulated by a unique-ouabain-insensitive cation pump.

Using Hyperactive Antifreeze Proteins To Supercool A Renal Tubule Cell Line

Cryobiology ◽  
2021 ◽  
Vol 103 ◽  
pp. 181
Author(s):  
Heather E. Tomalty ◽  
Virginia K. Walker ◽  
Peter L. Davies
Keyword(s):  
Cell Line ◽  
Renal Tubule ◽  
Antifreeze Proteins ◽  
Tubule Cell ◽  
Renal Tubule Cell

Proteomics Based Study of Soybean and Phakopsora pachyrhizi Interaction

2013 ◽  
Vol 14 (1) ◽  
pp. 29 ◽  
Author(s):  
M. Ganiger ◽  
D. R. Walker ◽  
Z. Y. Chen
Keyword(s):  
Gel Electrophoresis ◽  
Time Course ◽  
Molecular Level ◽  
Protein Profile ◽  
Differentially Expressed ◽  
Soybean Rust ◽  
Phakopsora Pachyrhizi ◽  
Yield Losses ◽  
Resistant Line ◽  
Differentially Expressed Protein

Phakopsora pachyrhizi, the causal agent of Asian soybean rust (ASR), has the potential to cause severe yield losses as all currently grown U.S. commercial soybean varieties are susceptible. In this proteomics study, we compared two soybean sibling lines, a resistant line 10G18 and a susceptible line 10G21 derived from Recombinant Inbred Line (RIL) population RN06-32-2 to understand the compatible and incompatible host-pathogen interactions at the molecular level. We compared the protein profile differences between the two lines over a time-course of 14 days with or without P. pachyrhizi inoculation using differential in-gel electrophoresis (DIGE). Approximately 70 differentially expressed spots between 10G18 and 10G21 lines with and without P. pachyrhizi inoculation were identified. Some of these spots, which were up- and down-regulated in resistant line 10G18, were sequenced using LC-MS/MS. Of the 70 differentially expressed protein spots, 31 up-regulated and 6 down-regulated spots in resistant line 10G18 were sequenced. These sequenced proteins were mostly involved in photosynthesis based on homology searches. The involvement in disease resistance for some of these differentially up-regulated proteins has been reported, indicating their possible role in soybean defense against ASR. However, further studies are necessary. Accepted for publication 3 September 2013. Published 25 November 2013.

The Role of Nitric Oxide and Vasp in Cytokine-Induced Renal Tubule Cell Shedding

2003 ◽  
Vol 104 (s49) ◽  
pp. 55P-55P
Author(s):  
Susan M. Crail ◽  
Thomas J. Evans
Keyword(s):  
Nitric Oxide ◽  
Renal Tubule ◽  
Tubule Cell ◽  
Renal Tubule Cell ◽  
Cell Shedding
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