Perivascular Nitric Oxide Delivery to Saphenous Vein Grafts Prevents Graft Stenosis after Coronary Artery Bypass Grafting: A Novel Sheep Model

Cardiology ◽  
2011 ◽  
Vol 118 (1) ◽  
pp. 8-15 ◽  
Author(s):  
Kyomars Abbasi ◽  
Keivan Shalileh ◽  
Maryam Sotudeh Anvari ◽  
Shahram Rabbani ◽  
Abolfazl Mahdanian ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Coronary Artery ◽  
Coronary Artery Bypass Grafting ◽  
Saphenous Vein ◽  
Artery Bypass ◽  
Bypass Grafting ◽  
Sheep Model ◽  
Vein Grafts ◽  
Graft Stenosis ◽  
Nitric Oxide Delivery

Comparison of Conventional and No-Touch Techniques in Harvesting Saphenous Vein for Coronary Artery Bypass Grafting in View of Endothelial Damage

2013 ◽  
Vol 16 (4) ◽  
pp. 177 ◽  
Author(s):  
Onur Sen ◽  
Suheyla Gonca ◽  
Seyhun Solakoglu ◽  
Hakki Dalcik ◽  
Cannur Dalcik ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Coronary Artery ◽  
Coronary Artery Bypass Grafting ◽  
Coronary Artery Bypass ◽  
Saphenous Vein ◽  
Artery Bypass ◽  
Surrounding Tissue ◽  
Bypass Grafting ◽  
Vein Wall ◽  
Immunohistochemical Assessment

<p><b>Background:</b> Optimization of saphenous vein patency for myocardial revascularization.</p><p><b>Objective:</b> The goal of this study was to present the no-touch technique of saphenous vein preparation. This technique consists of harvesting the vein with a pedicle of surrounding tissue, which protects the vein from distension pressure.</p><p><b>Methods:</b> We performed a prospective, randomized study that compared 2 techniques for harvesting saphenous vein-conventional and no-touchin 40 patients undergoing coronary artery bypass grafting. We carried out a morphologic study of the endothelium with the aid of light and transmission electron microscopy and an immunohistochemical assessment to identify adenosine, inducible nitric oxide synthase (iNOS), and vascular endothelial growth factor (VEGF) in the vein wall.</p><p><b>Results:</b> The integrity of endothelial cell and all vascular layers was maintained better with the no-touch technique than with the conventional procedure. The immunohistochemical assessment revealed that adenosine receptor, iNOS, and VEGF immunoexpression levels were normal or lower in the no-touch group than in the conventional-harvest group, as shown by the staining densities in all layers of the vein wall.</p><p><b>Conclusion:</b> Endothelial integrity and adenosine, iNOS, and VEGF immunoreactivities were better preserved when the no-touch technique was used for vein graft harvesting. The mechanical protection provided by the cushion of surrounding tissue in the no-touch group and the vasorelaxation and thromboresistant activities of nitric oxide may be responsible for the reduction in vasospasms and the improved patency rate.</p>

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