scholarly journals Development and Validation of a Method for Densitometric Analysis of Berberine in Herbal Extract and Polyherbal Formulation

2010 ◽  
Vol 19 (6) ◽  
pp. 473-478 ◽  
Author(s):  
V.K. Ghosh ◽  
D.H. Nagore ◽  
M.J. Patil ◽  
A. Prakash
Keyword(s):  
Herbal Extract ◽  
Densitometric Analysis ◽  
Polyherbal Formulation ◽  
Development And Validation

scholarly journals DEVELOPMENT AND VALIDATION OF A HPTLC METHOD FOR SIMULTANEOUS DENSITOMETRIC ANALYSIS OF GLYCYRRHETIC ACID AND SOLASODINE IN HERBAL DRUG FORMULATION

2017 ◽  
Vol 9 (4) ◽  
pp. 29 ◽  
Author(s):  
Kinjal Patel ◽  
Chaitanya Bhatt
Keyword(s):  
High Performance ◽  
Area Under The Curve ◽  
Retention Factor ◽  
Drug Formulation ◽  
Glycyrrhetic Acid ◽  
Densitometric Analysis ◽  
Polyherbal Formulation ◽  
Quality Control Tool ◽  
Development And Validation ◽  
Hptlc Method

Objective: To develop and validate a simple, precise, selective, and accurate high-performance thin layer chromatographic method for simultaneous densitometric analysis of glycyrrhetic acid and solasodine in the polyherbal formulation.Methods: The method was developed using HPTLC silica gel GF254precoated aluminium plate as the stationary phase and Chloroform: Methanol (9:1 v/v) as the mobile phase. Quantization of glycyrrhetic acid was achieved by determining the area under the curve at 267 nm using CAMAG TLC Scanner and CATS 3 software. Since the structure of solasodine lacks conjugated double bond, it does not give any fluorescence either in the absorbance mode or reflectance mode hence solasodine was derivatized using 0.5%anisaldehyde sulphuric acids which gave a bluish spot as seen on TLC plate. These spots were scanned at 546 nm wavelength using CAMAG TLC Scanner and CATS 3 software.Results: The retention factor for glycyrrhetic acid and solasodine were found to be 0.52±0.01, 0.40±0.01% w/w respectively. The developed HPTLC method was validated using parameters described in International Conference on Harmonization (ICH) guideline. The proposed method showed good linearity in the range of 400-2000 ng spot-1 for glycyrrhetic acid as well as for solasodine. The content of glycyrrhetic acid and solasodine in marketed polyherbal formulation were found to be 0.67%±0.8 and 0.10±0.35%w/w respectively.Conclusion: The developed method can be used as quality control tool for the routine analysis of glycyrrhetic acid and solasodine in polyherbal formulation.

Development and validation of a method for simultaneous densitometric analysis of simvastatin and ezetimibe as the bulk drugs and in the tablet dosage form

2008 ◽  
Vol 20 (1) ◽  
pp. 71-79 ◽  
Author(s):  
S. Dhaneshwar Dhaneshwar ◽  
P. Deshpande ◽  
M. Patil ◽  
G. Vadnerkar ◽  
S. Dhaneshwar Dhaneshwar
Keyword(s):  
Dosage Form ◽  
Densitometric Analysis ◽  
Development And Validation ◽  
Tablet Dosage

Efinaconazole: DoE-supported development and validation of a quantitative HPTLC method and its application for the assay of drugs in solution and microemulsion-based formulations

2020 ◽  
Vol 12 (10) ◽  
pp. 1380-1388 ◽  
Author(s):  
Rashmin B. Patel ◽  
Mrunali R. Patel ◽  
Nikesh R. Patni ◽  
Vikas Agrawal
Keyword(s):  
Pharmaceutical Formulations ◽  
Densitometric Analysis ◽  
Development And Validation ◽  
Hptlc Method

Herein, a simple and rapid HPTLC method using densitometric analysis was developed and validated for the assay of efinaconazole in pharmaceutical formulations (solution and microemulsion).

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