Isolation and Characterization of Naturally Processed Peptides Bound by Class II Molecules and Peptides Presented by Normal and Mutant Antigen-Presenting Cells

1993 ◽  
pp. 152-165 ◽  
Author(s):  
Alessandro Sette ◽  
Robert DeMars ◽  
Howard M. Grey ◽  
Carla Oseroff ◽  
Scott Southwood ◽  
...  
Keyword(s):  
Antigen Presenting Cells ◽  
Class Ii ◽  
Isolation And Characterization ◽  
Antigen Presenting

Allotransplantation of culture-isolated neonatal rat islet tissue. Absence of MHC class II positive antigen-presenting cells in nonimmunogenic islets

Diabetes ◽  
1989 ◽  
Vol 38 (2) ◽  
pp. 146-151 ◽  
Author(s):  
O. D. Hegre ◽  
R. J. Ketchum ◽  
H. Popiela ◽  
C. R. Eide ◽  
R. M. Meloche ◽  
...  
Keyword(s):  
Mhc Class Ii ◽  
Antigen Presenting Cells ◽  
Class Ii ◽  
Neonatal Rat ◽  
Islet Tissue ◽  
Antigen Presenting

scholarly journals Stratification of Antigen-presenting Cells within the Normal Cornea

2009 ◽  
Vol 1 ◽  
pp. OED.S2813 ◽  
Author(s):  
Jared E. Knickelbein ◽  
Simon C. Watkins ◽  
Paul G. Mcmenamin ◽  
Robert L. Hendricks
Keyword(s):  
Bone Marrow ◽  
Basement Membrane ◽  
Mhc Class Ii ◽  
Fluorescent Protein ◽  
Antigen Presenting Cells ◽  
Class Ii ◽  
Apical Surface ◽  
Bone Marrow Derived Cells ◽  
Green Fluorescent ◽  
Antigen Presenting

The composition and location of professional antigen presenting cells (APC) varies in different mucosal surfaces. The cornea, long considered an immune-privileged tissue devoid of APCs, is now known to host a heterogeneous network of bone marrow-derived cells. Here, we utilized transgenic mice that express enhanced green fluorescent protein (EGFP) from the CD 11c promoter (pCD11c) in conjunction with immunohistochemical staining to demonstrate an interesting stratification of APCs within non-inflamed murine corneas. pCD11c+ dendritic cells (DCs) reside in the basal epithelium, seemingly embedded in the basement membrane. Most DCs express MHC class II on at least some dendrites, which extend up to 50 μm in length and traverse up 20 μm tangentially towards the apical surface of the epithelium. The DC density diminishes from peripheral to central cornea. Beneath the DCs and adjacent to the stromal side of the basement membrane reside pCD11c–CD11b+ putative macrophages that express low levels of MHC class II. Finally, MHC class II–pCD11c–CD11b+ cells form a network throughout the remainder of the stroma. This highly reproducible stratification of bone marrow-derived cells is suggestive of a progression from an APC function at the exposed corneal surface to an innate immune barrier function deeper in the stroma.

Characterization of antigen-presenting cells in human apical periodontitis lesions by flow cytometry and immunocytochemistry

2006 ◽  
Vol 39 (8) ◽  
pp. 626-636 ◽  
Author(s):  
A. Lukic ◽  
S. Vasilijic ◽  
I. Majstorovic ◽  
D. Vucevic ◽  
S. Mojsilovic ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Antigen Presenting Cells ◽  
Apical Periodontitis ◽  
Antigen Presenting

Endocytosis and Biosynthetic Transport of Murine MHC Class II Molecules in Antigen Presenting Cells

Endocytosis ◽  
1992 ◽  
pp. 341-342
Author(s):  
J. Davoust ◽  
P. Cosson ◽  
J. M. Escola ◽  
J. Henry ◽  
M. Humbert ◽  
...  
Keyword(s):  
Mhc Class Ii ◽  
Antigen Presenting Cells ◽  
Class Ii ◽  
Antigen Presenting ◽  
Mhc Class Ii Molecules

scholarly journals Isolation and characterization of the intracellular MHC class II compartment

Nature ◽  
1994 ◽  
Vol 369 (6476) ◽  
pp. 120-126 ◽  
Author(s):  
Abraham Tulp ◽  
Desirée Verwoerd ◽  
Bernhard Dobberstein ◽  
Hidde L. Ploegh ◽  
Jean Pieters
Keyword(s):  
Mhc Class Ii ◽  
Class Ii ◽  
Isolation And Characterization
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