Genotypic and Phenotypic Variation of HIV-1: Impact on AIDS Pathogenesis and Vaccination (Part 1 of 2)

Chemical Immunology and Allergy - Immunochemistry of AIDS ◽

10.1159/000319152 ◽

1993 ◽

pp. 1-17 ◽

Cited By ~ 3

Author(s):

Tom F.W. Wolfs ◽

Peter L. Nara ◽

Jaap Goudsmit

Keyword(s):

Phenotypic Variation ◽

Hiv 1

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Genotypic and Phenotypic Variation of HIV-1: Impact on AIDS Pathogenesis and Vaccination (Part 2 of 2)

Chemical Immunology and Allergy - Immunochemistry of AIDS ◽

10.1159/000319154 ◽

1993 ◽

pp. 18-33

Author(s):

Tom F.W. Wolfs ◽

Peter L. Nara ◽

Jaap Goudsmit

Keyword(s):

Phenotypic Variation ◽

Hiv 1

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Genotypic and Phenotypic Variation of HIV-1: Impact on AIDS Pathogenesis and Vaccination

Chemical Immunology and Allergy - Immunochemistry of AIDS ◽

10.1159/000421945 ◽

2015 ◽

pp. 1-33

Author(s):

Tom F. W. Wolfs ◽

Peter L. Nara ◽

Jaap Goudsmit

Keyword(s):

Phenotypic Variation ◽

Hiv 1

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Role of R5 phenotypic variation in mother-to-child transmission of HIV-1

Retrovirology ◽

10.1186/1742-4690-5-s1-o2 ◽

2008 ◽

Vol 5 (Suppl 1) ◽

pp. O2

Author(s):

Mariangela Cavarelli ◽

Ingrid Karlsson ◽

Marisa Zanchetta ◽

Liselotte Antonsson ◽

Anna Plebani ◽

...

Keyword(s):

Phenotypic Variation ◽

Mother To Child Transmission ◽

Child Transmission ◽

Mother To Child ◽

Hiv 1

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Ultrastructural observations of human monocytes infected with HIV-1

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100084843 ◽

1991 ◽

Vol 49 ◽

pp. 108-109

Author(s):

James K. Koehler ◽

Steven G. Reed ◽

Joao S. Silva

Keyword(s):

Gradient Centrifugation ◽

Virus Production ◽

Human Monocyte ◽

Red Cross ◽

Human Monocytes ◽

Blood Monocytes ◽

Hiv Replication ◽

Electron Microscopic ◽

Ultrastructural Studies ◽

Hiv 1

As part of a larger study involving the co-infection of human monocyte cultures with HIV and protozoan parasites, electron microscopic observations were made on the course of HIV replication and infection in these cells. Although several ultrastructural studies of the cytopathology associated with HIV infection have appeared, few studies have shown the details of virus production in “normal,” human monocytes/macrophages, one of the natural targets of the virus, and suspected of being a locus of quiescent virus during its long latent period. In this report, we detail some of the interactions of developing virons with the membranes and organelles of the monocyte host.Peripheral blood monocytes were prepared from buffy coats (Portland Red Cross) by Percoll gradient centrifugation, followed by adherence to cover slips. 90-95% pure monocytes were cultured in RPMI with 5% non-activated human AB serum for four days and infected with 100 TCID50/ml of HIV-1 for four hours, washed and incubated in fresh medium for 14 days.

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