Mechanism of Action: In vitro Studies

1986 ◽  
pp. 198-221 ◽  
Author(s):  
Allan D. Hess ◽  
Paul M. Colombani
Keyword(s):  
Mechanism Of Action ◽  
In Vitro Studies

scholarly journals Network pharmacology-based analysis for unraveling potential cancer-related molecular targets of Egyptian propolis phytoconstituents accompanied with molecular docking and in vitro studies

RSC Advances ◽  
2021 ◽  
Vol 11 (19) ◽  
pp. 11610-11626
Author(s):  
Reham S. Ibrahim ◽  
Alaa A. El-Banna
Keyword(s):  
Molecular Docking ◽  
Cancer Treatment ◽  
Mechanism Of Action ◽  
Molecular Targets ◽  
Integrated Approach ◽  
In Vitro Studies ◽  
Network Pharmacology ◽  
Multi Level ◽  
Potential Cancer

Multi-level mechanism of action of propolis constituents in cancer treatment using an integrated approach of network pharmacology-based analysis, molecular docking and in vitro cytotoxicity testing.

The acute hypocalcaemic effect of ethanol and its mechanism of action in the rat

1983 ◽  
Vol 61 (4) ◽  
pp. 388-394 ◽  
Author(s):  
Nateetip Krishnamra ◽  
Liangchai Limlomwongse
Keyword(s):  
Body Weight ◽  
Mechanism Of Action ◽  
Calcium Release ◽  
Calcium Influx ◽  
Plasma Calcium ◽  
In Vitro Studies ◽  
Calcium Efflux ◽  
Plasma Pool ◽  
Suppressive Action

The hypocalcaemic action of ethanol (3 g/kg body weight) was investigated in intact, thyroparathyroidectomized and antrectomized rats. It was found that ethanol administered either intraperitoneally or orally reduced plasma calcium concentrations within 30 min and that this response lasted for 8 h. Additional studies performed in antrectomized and thyroparathyroidectomized rats indicated that neither gastrin nor the hormones parathormone and calcitonin had any effect on the hypocalcaemic effect of ethanol. Investigation of the mechanism of action of ethanol-induced hypocalcaemia involved measurements of calcium efflux from and influx into the plasma pool. Ethanol did not have any effect on the disappearance from plasma of 45Ca administered intravenously at 0 min. In contrast, ethanol was found to enhance the disappearance of 45Ca administered intraperitoneally 17 h prior to the experiment. The interpretation of 45Ca studies was discussed and it was concluded that ethanol-induced hypocalcaemia resulted from a decrease in calcium influx into the plasma. Additional in vitro studies did not indicate the suppressive action of ethanol on the release of calcium from tibias. In conclusion, our results show that the mechanism of hypocalcaemia caused by ethanol is the suppression of calcium release from some tissue(s) into the plasma.

In vitro studies on the mechanism of action of a new antiallergic, Ro 21-7634

1981 ◽  
Vol 11 (4) ◽  
pp. 345-351 ◽  
Author(s):  
Ann F. Welton ◽  
William C. Hope ◽  
Herman J. Crowley ◽  
Richard A. Salvador
Keyword(s):  
Mechanism Of Action ◽  
In Vitro Studies

In Vitro Studies on Erythrophagocytosis

Blood ◽  
1963 ◽  
Vol 22 (3) ◽  
pp. 295-312 ◽  
Author(s):  
ROBERT M. GREENDYKE ◽  
ROBERT E. BRIERTY ◽  
SCOTT M. SWISHER ◽  
Norma Trabold ◽  
Richard Ryser
Keyword(s):  
Energy Metabolism ◽  
Quantitative Study ◽  
Mechanism Of Action ◽  
The State ◽  
In Vitro Studies ◽  
Physical Factors ◽  
Factors Influencing ◽  
Physical Chemical ◽  
Two Systems

Abstract 1. Two systems for the quantitative study of erythrophagocytosis in vitro are described. 2. The effects of a number of physical factors, cations, anticoagulants, and metabolic poisons in these systems are reported. 3. Existing views on the mechanism of action of factors influencing phagocytosis are briefly reviewed. The importance of the state of energy metabolism of the phagocyte is stressed. 4. The importance of physical, chemical, and metabolic aspects of erythrophagocytic systems is emphasized in connection with the use of erythrophagocytosis as a serologic tool in immunohematology.

scholarly journals Molecular Mechanisms of Mitotane Action in Adrenocortical Cancer Based on In Vitro Studies

Cancers ◽  
2021 ◽  
Vol 13 (21) ◽  
pp. 5255
Author(s):  
Marco Lo Iacono ◽  
Soraya Puglisi ◽  
Paola Perotti ◽  
Laura Saba ◽  
Jessica Petiti ◽  
...  
Keyword(s):  
Cell Lines ◽  
Mechanism Of Action ◽  
Molecular Mechanisms ◽  
Cytochromes P450 ◽  
Culture Conditions ◽  
In Vitro Studies ◽  
Adrenocortical Cancer ◽  
Experimental Conditions ◽  
Cell Strains

Mitotane is the only approved drug for the treatment of advanced adrenocortical carcinoma and is increasingly used for postoperative adjuvant therapy. Mitotane action involves the deregulation of cytochromes P450 enzymes, depolarization of mitochondrial membranes, and accumulation of free cholesterol, leading to cell death. Although it is known that mitotane destroys the adrenal cortex and impairs steroidogenesis, its exact mechanism of action is still unclear. The most used cell models are H295-derived cell strains and SW13 cell lines. The diverging results obtained in presumably identical cell lines highlight the need for a stable in vitro model and/or a standard methodology to perform experiments on H295 strains. The presence of several enzymatic targets responsive to mitotane in mitochondria and mitochondria-associated membranes causes progressive alteration in mitochondrial structure when cells were exposed to mitotane. Confounding factors of culture affecting in vitro experiments could reduce the significance of any molecular mechanism identified in vitro. To ensure experimental reproducibility, particular care should be taken in the choice of culture conditions: aspects such as cell strains, culture serum, lipoproteins concentration, and culture passages should be carefully considered and explicated in the presentation of results. We aimed to review in vitro studies on mitotane effects, highlighting how different experimental conditions might contribute to the controversial findings. If the concerns pointed out in this review will be overcome, the new insights into mitotane mechanism of action observed in-vitro could allow the identification of novel pharmacological molecular pathways to be used to implement personalized therapy.

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