Fluorescent Microsatellite Analysis of Urine Sediment in Patients with Urothelial Carcinoma

2010 ◽  
Vol 85 (3) ◽  
pp. 296-303 ◽  
Author(s):  
Jian-Fang Liang ◽  
Hui-Xia Zheng ◽  
Ning Li ◽  
Yan Yin ◽  
Cai-Xia Cheng ◽  
...  
Keyword(s):  
Urothelial Carcinoma ◽  
Microsatellite Analysis ◽  
Urine Sediment ◽  
Analysis Of Urine

Microsatellite analysis of urine sediment versus urine cytology for diagnosing transitional cell tumors of the urinary bladder

Apmis ◽  
2004 ◽  
Vol 112 (2) ◽  
pp. 148-152 ◽  
Author(s):  
DELFINA FORNARI ◽  
KENNETH STEVEN ◽  
ALASTAIR B. HANSEN ◽  
HENRIK VIBITS ◽  
JAN V. JEPSEN ◽  
...  
Keyword(s):  
Urinary Bladder ◽  
Transitional Cell ◽  
Microsatellite Analysis ◽  
Urine Cytology ◽  
Urine Sediment ◽  
Transitional Cell Tumors ◽  
Analysis Of Urine

Improved Filter Method for Urine Sediment Detection of Urothelial Carcinoma by Fluorescence In Situ Hybridization

2007 ◽  
Vol 131 (10) ◽  
pp. 1574-1577 ◽  
Author(s):  
Isabelle Meiers ◽  
Harpreet Singh ◽  
Deloar Hossain ◽  
Kevin Lang ◽  
Lina Liu ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Urothelial Carcinoma ◽  
Urine Cytology ◽  
Filter Method ◽  
Urine Sediment ◽  
Specific Test ◽  
Significant Difference ◽  
Time Required

AbstractContext.—Fluorescence in situ hybridization (FISH) of voided urine sediment is a sensitive and specific test for the detection of urothelial carcinoma. The time required for slide preparation using the conventional cytospin method is lengthy.Objective.—To present an alternative to the conventional cytospin method.Design.—We compared the results of an improved filter monolayer method with published results of the conventional cytospin method. A total of 624 patients with cytology and FISH analyses were followed with cystoscopy and/ or bladder biopsy. Fluorescence in situ hybridization analysis was performed on 624 cases using fluorescence-labeled probes to the pericentromeric regions of chromosomes 3, 7, and 17 and band 9p21; cytology was also performed in all cases.Results.—A total of 217 (34.7%) of 624 patients had follow-up bladder biopsies, and 170 of these (78.3%) had urothelial carcinoma. The sensitivity for cancer detection was higher for FISH than for urine cytology (92.9% [158/ 170] for FISH vs 72.9% [124/170] for urine cytology, P = <5%). The specificity was equivalent for FISH and urine cytology (97.5% [443/454] for FISH vs 92.2% [419/454] for cytology). The sensitivity for FISH was better (92.9% vs 81%), and there was no significant difference in specificity (97.5% vs 96%) between the filter method and the conventional cytospin method. Unlike the conventional cytospin method, the filter method did not require multiple centrifugation and decantation steps or investment in dedicated equipment.Conclusions.—The improved filter method was faster, easier, and less expensive than published results with the conventional cytospin method with better sensitivity and equivalent specificity.

Detection of Bladder Cancer Recurrence by Microsatellite Analysis of Urine

1998 ◽  
Vol 159 (5) ◽  
pp. 1764-1765 ◽  
Author(s):  
G. Steiner ◽  
M.P. Schoenberg ◽  
J.F. Linn ◽  
L. Mao ◽  
D. Sidransky
Keyword(s):  
Bladder Cancer ◽  
Cancer Recurrence ◽  
Microsatellite Analysis ◽  
Bladder Cancer Recurrence ◽  
Analysis Of Urine

scholarly journals Analysis of Urine Sediment for Cytology and Antigen Expression in Acute Renal Allograft Rejection

2012 ◽  
Vol 137 (5) ◽  
pp. 816-824 ◽  
Author(s):  
Priti Chatterjee ◽  
Sandeep R. Mathur ◽  
Amit K. Dinda ◽  
Sandeep Guleria ◽  
Sandeep Mahajan ◽  
...  
Keyword(s):  
Allograft Rejection ◽  
Renal Allograft ◽  
Antigen Expression ◽  
Urine Sediment ◽  
Renal Allograft Rejection ◽  
Acute Renal Allograft Rejection ◽  
Analysis Of Urine

A New Chemical Preservative That Permits Analysis of Urine Sediment for Light Microscopic Examination 12 h after Emission

Nephron ◽  
1999 ◽  
Vol 82 (1) ◽  
pp. 65-71 ◽  
Author(s):  
M. del Rosario Rodríguez Moreno ◽  
Irene Rodríguez Moreno ◽  
M. Teresa Miranda León ◽  
Monique Boy ◽  
Nina Cowdry Agnieszka
Keyword(s):  
Microscopic Examination ◽  
Urine Sediment ◽  
Light Microscopic Examination ◽  
Chemical Preservative ◽  
Analysis Of Urine

Detection of bladder cancer recurrence by microsatellite analysis of urine

1997 ◽  
Vol 3 (6) ◽  
pp. 621-624 ◽  
Author(s):  
Gabriel Steiner ◽  
Mark P. Schoenberg ◽  
Jurgen F. Linn ◽  
Li Mao ◽  
David Sidransky
Keyword(s):  
Bladder Cancer ◽  
Cancer Recurrence ◽  
Microsatellite Analysis ◽  
Bladder Cancer Recurrence ◽  
Analysis Of Urine

Surveillance with Microsatellite Analysis of Urine in Bladder Cancer Patients Treated by Radiotherapy

2003 ◽  
Vol 43 (4) ◽  
pp. 369-373 ◽  
Author(s):  
B VANRHIJN ◽  
M SMIT ◽  
D VANGEENEN ◽  
A WIJNMAALEN ◽  
W KIRKELS ◽  
...  
Keyword(s):  
Bladder Cancer ◽  
Cancer Patients ◽  
Microsatellite Analysis ◽  
Analysis Of Urine
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