In vitro and in vivo Cytogenetic Effects of Recombinant Human Erythropoietin on the Frequency of Sister Chromatid Exchanges Alone or in Combination with Mitomycin C

Chemotherapy ◽  
2010 ◽  
Vol 56 (3) ◽  
pp. 239-247 ◽  
Author(s):  
E.N. Digkas ◽  
S. Chrisafi ◽  
T. Passadaki ◽  
A. Tsalkidis ◽  
A. Hatzimichail ◽  
...  
Keyword(s):  
Mitomycin C ◽  
Sister Chromatid ◽  
Recombinant Human Erythropoietin ◽  
Sister Chromatid Exchanges ◽  
Human Erythropoietin ◽  
Cytogenetic Effects ◽  
Chromatid Exchanges

scholarly journals PRESENCE OF A BASE LINE LEVEL OF SISTER CHROMATID EXCHANGES IN SOMATIC CELLS OF DROSOPHILA MELANOGASTER IN VIVO AND IN VITRO

Genetics ◽  
1982 ◽  
Vol 100 (2) ◽  
pp. 259-278
Author(s):  
Hideo Tsuji
Keyword(s):  
Drosophila Melanogaster ◽  
Sister Chromatid ◽  
Ganglion Cells ◽  
Sister Chromatid Exchanges ◽  
Base Line ◽  
Cell Cycles ◽  
Chromatid Exchanges ◽  
Third Instar Larvae

ABSTRACT Sister chromatid exchanges (SCEs) under in vivo and in vitro conditions were examined in ganglion cells of third-instar larvae of Drosophila melanogaster (Oregon-R). In the in vivo experiment, third-instar larvae were fed on synthetic media containing 5-bromo-2′-deoxyuridine (BrdUrd). After two cell cycles, ganglia were dissected and treated with colchicine. In the in vitro experiment, the ganglia were also incubated in media containing BrdUrd for two cell cycles, and treated with colchicine. SCEs were scored in metaphase stained with Hoechst 33258 plus Giemsa. The frequencies of SCEs stayed constant in the range of 25-150 vg/ml and 0.25-2.5 vg/ml of BrdUrd in vivo and in vitro, respectively. SCEs gradually increased at higher concentrations, strongly suggesting that at least a fraction of the detected SCEs are spontaneous. The constant levels of SCE frequency were estimated, on the average, at 0.103 per cell per two cell cycles for females and 0.101 for males in vivo and at 0.096 for females and 0.091 for males in vitro. No difference was found in the SCE frequency between sexes at any of the BrdUrd concentrations. The analysis for the distribution of SCEs within chromosomes revealed an extraordinarily high proportion of the SCEs at the junctions between euchromatin and heterochromatin; the remaining SCEs were preferentially localized in the euchromatic regions of the chromosomes and in the heterochromatic Y chromosome. These results were largely inconsistent with those of Gatti et al. (1979).

Sister chromatid exchanges induced in vitro and in vivo by an extract of black pepper

1997 ◽  
Vol 35 (6) ◽  
pp. 567-571 ◽  
Author(s):  
E. Madrigal-Bujaidar ◽  
S. Diaz Barriga ◽  
P. Mota ◽  
R. Guzman ◽  
M. Cassani
Keyword(s):  
Sister Chromatid ◽  
Black Pepper ◽  
Sister Chromatid Exchanges ◽  
Chromatid Exchanges
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