Pollen Enzymes Degrade Human Tear Fluid and Conjunctival Cells: An Approach to Understanding Seasonal Non-Allergic Conjunctivitis

2010 ◽  
pp. 83-92 ◽  
Author(s):  
Dieter Franz Rabensteiner ◽  
Eva Spreitzhofer ◽  
Gabriele Trummer ◽  
Christine Wachswender ◽  
Sieglinde Kirchengast ◽  
...  
Keyword(s):  
Allergic Conjunctivitis ◽  
Tear Fluid ◽  
Conjunctival Cells

Allergic conjunctivitis, total and specific IgE in the tear fluid

2009 ◽  
Vol 63 (1) ◽  
pp. 97-99 ◽  
Author(s):  
O. Kari ◽  
O. P. Salo ◽  
F. Björkstén ◽  
A. Backman
Keyword(s):  
Allergic Conjunctivitis ◽  
Specific Ige ◽  
Tear Fluid

Characterization of Cytokine mRNA Transcripts in Conjunctival Cells from Patients with Allergic Conjunctivitis

1995 ◽  
Vol 35 (4) ◽  
pp. 173
Author(s):  
H. Fujishima ◽  
K. Yamada ◽  
Y. Yagi ◽  
J. Shimazaki ◽  
I. Saito ◽  
...  
Keyword(s):  
Allergic Conjunctivitis ◽  
Cytokine Mrna ◽  
Mrna Transcripts ◽  
Conjunctival Cells

Specific IgE in Tear Fluid and Features of Allergic Conjunctivitis

2013 ◽  
Vol 38 (9) ◽  
pp. 917-925 ◽  
Author(s):  
Tatsuya Mimura ◽  
Satoru Yamagami ◽  
Yuko Kamei ◽  
Mari Goto ◽  
Masao Matsubara
Keyword(s):  
Allergic Conjunctivitis ◽  
Specific Ige ◽  
Tear Fluid

Characterization of Cytokine mRNA Transcripts in Conjunctival Cells from Patients with Allergic Conjunctivitis

1995 ◽  
Vol 35 (4) ◽  
pp. 173
Author(s):  
H Fujishima ◽  
K Yamada ◽  
Y Yagi ◽  
J Shimazaki ◽  
I Saito ◽  
...  
Keyword(s):  
Allergic Conjunctivitis ◽  
Cytokine Mrna ◽  
Mrna Transcripts ◽  
Conjunctival Cells

scholarly journals Altered hyaluronic acid content in tear fluid of patients with adenoviral conjunctivitis

2015 ◽  
Vol 87 (1) ◽  
pp. 455-462 ◽  
Author(s):  
JULIANA L. DREYFUSS ◽  
CAIO V. REGATIERI ◽  
BRUNO COELHO ◽  
JOSÉ B. BARBOSA ◽  
DENISE DE FREITAS ◽  
...  
Keyword(s):  
Allergic Conjunctivitis ◽  
Protein Profile ◽  
Clinical Signs ◽  
Tear Fluid ◽  
Specific Test ◽  
Tear Proteins ◽  
Viral Culture ◽  
Sds Page ◽  
The World ◽  
Acute Conjunctivitis

The adenoviral conjunctivitis is one of the biggest causes of conjunctival infection in the world. Conjunctivitis causes relatively nonspecific symptoms, as hyperaemia and chemosis. Even after biomicroscopy, complex laboratory tests, such as viral culture, are necessary to identify the pathogen or its etiology. To contribute to the better understanding of the pathobiology of the adenoviral conjunctivitis, the tear fluids of patients with unilateral acute adenovirus conjunctivitis (UAAC), normal donors (control) and patients with allergic conjunctivitis were analyzed. Tear samples were collected with Schirmer strips from control, allergic conjunctivitis and UAAC patients, diagnosed by clinical signs. UAAC tears were tested positive in viral cultures. After the elution, HA was quantified using an ELISA-like fluorometric assay and the protein profile was determined by SDS-PAGE. A profound increase in the HA tear content in UAAC patients was found when compared to control and ALC. This HA increase in UAAC tears remarkably was not observed in tears from contralateral eyes without clinical signs, nor in allergic conjunctivitis. In addition a distinct profile of UAAC tear proteins was observed in patients with UAAC. The quantification of HA in the tear fluid is a rapid, sensitive and specific test. This molecule might be a biomarker candidate for acute conjunctivitis.

Specific IgE for wheat in tear fluid of patients with allergic conjunctivitis

2014 ◽  
Vol 34 (1) ◽  
pp. 25-34 ◽  
Author(s):  
Tatsuya Mimura ◽  
Satoru Yamagami ◽  
Hidetaka Noma ◽  
Yuko Kamei ◽  
Mari Goto ◽  
...  
Keyword(s):  
Allergic Conjunctivitis ◽  
Specific Ige ◽  
Tear Fluid

scholarly journals Expression of TSLP and Downstream Molecules IL-4, IL-5, and IL-13 on the Eye Surface of Patients with Various Types of Allergic Conjunctivitis

2016 ◽  
Vol 2016 ◽  
pp. 1-7 ◽  
Author(s):  
Xiaofen Zheng ◽  
Juan Yao ◽  
Bing Li
Keyword(s):  
Allergic Conjunctivitis ◽  
Rna Extraction ◽  
Cross Sectional Study ◽  
Vernal Keratoconjunctivitis ◽  
Cross Sectional ◽  
Seasonal Allergic Conjunctivitis ◽  
Significant Difference ◽  
Conjunctival Cells ◽  
Post Hoc ◽  
Normal Controls

Background.The pathogenesis of allergic conjunctivitis has not been clearly established. Moreover, previous studies fail to consider human models of allergic conjunctivitis. This study investigated the expression of thymic stromal lymphopoiet in TSLP and its downstream molecules in conjunctival scrappings and tear.Methods.This cross-sectional study compares patients with vernal keratoconjunctivitis (VKC), seasonal allergic conjunctivitis (SAC), and perennial allergic conjunctivitis (PAC) with normal controls. There are 80 people recorded in Shanxi Eye Hospital. Increasingly, 20 are with VKC, 20 are with SAC, 20 are with PAC, and the remaining 20 are normal controls. Conjunctiva were harvested for total RNA extraction and gene expression by real-time polymerase chain reaction. Epithelial cells were collected to make pathological sections for immunohistochemical staining. Human tears were evaluated by Luminex microbead assay. APvalue less than 0.05 from Dunnett’s post hoc test in SPSS means a statistical significant distinction.Results.Positive expression in conjunctival cells of patients with allergic conjunctivitis. The expression of TSLP and IL-4, IL-5, and IL-13 mRNA shows a statistically significant difference (P<0.05). TSLP and IL-4, IL-5, and IL-13 concentrations show a statistically significant difference (P<0.01).Conclusions.This study suggests that TSLP and downstream molecules are expressed in patients with various types of allergic conjunctivitis.

Platelet Activating Factor Degradation in Tear Fluid from Guinea Pigs with Allergic Conjunctivitis

2001 ◽  
Vol 17 (1) ◽  
pp. 83-91 ◽  
Author(s):  
Masatomo Kato ◽  
Hidetoshi Mano ◽  
Atsutoshi Ota ◽  
Koji Konomi ◽  
Katsuhiko Nakata
Keyword(s):  
Guinea Pigs ◽  
Allergic Conjunctivitis ◽  
Platelet Activating Factor ◽  
Tear Fluid

scholarly journals Elevated levels of prostaglandin E2 in the tears of patients with severe allergic conjunctivitis and primary cultured conjunctival cells are suppressed by ketotifen and dexamethasone

2021 ◽  
Vol 6 (1) ◽  
pp. e000571
Author(s):  
Ryutaro Yamanishi ◽  
Naoko Okada ◽  
Eisuke Shimizu ◽  
Hiroshi Fujishima
Keyword(s):  
Allergic Conjunctivitis ◽  
Primary Cultures ◽  
Allergic Inflammation ◽  
Prostaglandin E ◽  
Eye Drops ◽  
Ketotifen Fumarate ◽  
Tnf Α ◽  
Conjunctival Cells ◽  
Culture Supernatants ◽  
Necrosis Factor

ObjectiveWe examined the production of prostaglandin E2 (PGE2), which is the key prostaglandin involved in inflammatory disorders of the ocular surface. Tears and conjunctival fibroblasts were evaluated in order to assess allergic inflammation and the effect of specific drugs.Methods and analysisPGE2 was measured in tears from both patients and normal volunteers. Primary cultures of human conjunctival fibroblasts were incubated with interleukin (IL)-4 and tumour necrosis factor (TNF)-α with or without ketotifen fumarate or dexamethasone. The culture supernatants were removed 24 hours after exposure and the concentrations of PGE2 were quantified by ELISA.ResultsSignificantly higher levels of PGE2 were observed in the tears of patients with severe allergic conjunctivitis than in those with post-surgical inflammation (p=0.02), and this production was reduced by eye drops. Stimulation with IL-4 and TNF-α induced the generation of PGE2 in supernatants of conjunctival fibroblasts, and this production was significantly downregulated by ketotifen fumarate or steroids.ConclusionPGE2 may participate in the pathogenesis of severe ocular allergic disease, and both ketotifen fumarate and steroid reduce the production of PGE2.

Elevated levels of substance P in tears of patients with allergic conjunctivitis and vernal keratoconjunctivitis

1997 ◽  
Vol 27 (4) ◽  
pp. 372-378 ◽  
Author(s):  
H. FUJISHIMA ◽  
M. TAKEYAMA ◽  
T. TAKEUCHI ◽  
I. SAITO ◽  
K. TSUBOTA
Keyword(s):  
Substance P ◽  
Allergic Conjunctivitis ◽  
Vernal Keratoconjunctivitis
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