The Physical Ability of Japanese Female Elderly with Cerebrovascular Disease Correlates with the Telomere Length and Subtelomeric Methylation Status in Their Peripheral Blood Leukocytes

Gerontology ◽  
2011 ◽  
Vol 57 (2) ◽  
pp. 137-143 ◽  
Author(s):  
Toyoki Maeda ◽  
Jun-ichi Oyama ◽  
Yoshihiro Higuchi ◽  
Yasuhiro Nishiyama ◽  
Yoshihiro Kudo ◽  
...  
Keyword(s):  
Cerebrovascular Disease ◽  
Telomere Length ◽  
Peripheral Blood ◽  
Methylation Status ◽  
Peripheral Blood Leukocytes ◽  
Blood Leukocytes ◽  
Physical Ability ◽  
Japanese Female

Low THRB (thyroid hormone receptor beta) Promoter Methylation Levels in Peripheral Blood Leukocytes Induced By Systematic Inflammation Are Involved in Low Thyroid Hormone Function in Metabolic Syndrome

Hypertension ◽  
2021 ◽  
Author(s):  
Bing Cui ◽  
Xiao Xiao ◽  
Jin’e Wang ◽  
Hongrui Wang ◽  
Cunjin Wu ◽  
...  
Keyword(s):  
Metabolic Syndrome ◽  
Thyroid Hormone ◽  
Promoter Methylation ◽  
Peripheral Blood ◽  
Thyroid Hormone Receptor ◽  
Oral Treatment ◽  
Methylation Status ◽  
Peripheral Blood Leukocytes ◽  
Blood Leukocytes ◽  
A Genome

Abnormal thyroid hormone (TH) function has been observed in all components of metabolic syndrome (MetS), but the mechanisms remain unclear. Altered genomic methylation status is closely related to MetS. Our aim was to determine whether methylation regulation in TH function–related genes is involved in MetS. In a small strictly selected cohort, low TH function was observed in MetS group, as well as lower THRB promoter methylation levels in peripheral blood leukocytes in a genome-wide methylation screening by Illumina 450K beadchip. The results of beadchip assay were then confirmed by Sequenom MassARRAY. Low THRB promoter methylation levels and low TH function in MetS were confirmed in another big-size validation cohort. Lower methylation levels were associated with higher THRB expression in peripheral blood leukocytes, and altered THRB promoter methylation status influenced its promoter activity and expression. In the MetS rat models constructed by high fat and high fructose diet, lower TH function was also observed, as well as lower Thrb promoter methylation levels. Furthermore, systematic inflammation observed in MetS was found to induce decreased THRB promoter methylation levels as well as corresponding THRB expression. Additionally, oral treatment with a physiological T3 dose mitigated hypertension and insulin resistance and partially alleviated hepatic steatosis and adipocyte hypertrophy in MetS rats. Low methylation levels of THRB promoter in peripheral blood leukocytes induced by systematic inflammation were involved in low TH function in MetS, whereas low TH function deteriorates MetS. This might serve as a novel therapeutic target of MetS.

Short telomere length in peripheral blood leukocytes in head and neck cancer: Findings in a Brazilian cohort

Head & Neck ◽  
2018 ◽  
Vol 41 (3) ◽  
pp. 672-677 ◽  
Author(s):  
Raquel M Alves-Paiva ◽  
Fernanda Gutierrez-Rodrigues ◽  
Diego A Pereira-Martins ◽  
David Livingstone Alves Figueiredo ◽  
Diego V Clé ◽  
...  
Keyword(s):  
Head And Neck Cancer ◽  
Head And Neck ◽  
Telomere Length ◽  
Neck Cancer ◽  
Peripheral Blood ◽  
Short Telomere ◽  
Peripheral Blood Leukocytes ◽  
Blood Leukocytes ◽  
Brazilian Cohort

Shorter Telomere Length in Peripheral Blood Leukocytes Is Associated with Post-Traumatic Chronic Osteomyelitis

2020 ◽  
Vol 21 (9) ◽  
pp. 773-777
Author(s):  
Songyun Deng ◽  
Shengyao Liu ◽  
Shaoyong Xu ◽  
Yongbin He ◽  
Xia Zhou ◽  
...  
Keyword(s):  
Telomere Length ◽  
Peripheral Blood ◽  
Chronic Osteomyelitis ◽  
Peripheral Blood Leukocytes ◽  
Blood Leukocytes ◽  
Post Traumatic

Telomere Length of Peripheral Blood Leukocytes Predicts Relapse and Clonal Evolution after Immunosuppressive Therapy in Severe Aplastic Anemia

Blood ◽  
2008 ◽  
Vol 112 (11) ◽  
pp. 442-442 ◽  
Author(s):  
James N Cooper ◽  
Rodrigo Calado ◽  
Colin Wu ◽  
Phillip Scheinberg ◽  
Neal Young
Keyword(s):  
Stem Cell ◽  
Cell Division ◽  
Aplastic Anemia ◽  
Telomere Length ◽  
Peripheral Blood ◽  
Copy Number ◽  
Clonal Evolution ◽  
Peripheral Blood Leukocytes ◽  
Blood Leukocytes ◽  
Hematopoietic Stem

Abstract Severe aplastic anemia (SAA) can be treated with either immunosuppressive therapy (IST) or hematopoietic stem cell transplantation (HSCT). Response to IST depends on control of the immune response and on a hematopoietic stem cell (HSC) compartment capable of repopulating the bone marrow after therapy. Telomeres are protective repetitive DNA sequences at the ends of chromosomes; telomeres shorten with each cell division due to DNA polymerase deficiency to fully duplicate telomeric ends. Cells with critically shortened telomeres undergo proliferative senescence, apoptosis, and genomic instability. Excessive shortening of telomeres has been proposed as a potential biomarker for HSC exhaustion, and loss-of-function mutations in telomerase genes cause excessive telomere erosion and associate with marrow failure. To test the hypothesis that telomere length is a predictor of successful outcome after IST in SAA, telomere length of peripheral blood leukocytes were measured by quantitative PCR and expressed as the relative ratio of telomere repeat copy number to single gene copy number (T/S ratio) in 168 consecutive patients prior to IST therapy. The cohort consisted of patients with SAA (mean age, 34 years; range, 4–82) enrolled in three sequential protocols at the National Institutes of Health from 2003 to 2008 (ClinicalTrials.gov identifiers, NCT00001964, NCT00260689, and NCT00061360); all patients received IST based on horse anti-thymoglobulin (h-ATG) plus cyclosporine (CsA). Additional treatments depended on specific protocol arm: mycophenolate mofetil (32 patients), rabbit-ATG in place of h-ATG (31 patients), or rapamycin (35 patients); 70 patients received h-ATG and CsA only. None of the patients had clinical findings suggestive of dyskeratosis congenita. All adult patients or legal guardians signed informed consent according to NHLBI Institutional Review Board. Response, relapse, and clonal evolution rates were similar across all regimens. Using both a univariate and multivariate Cox regression model, telomere length was not associated with response (partial or complete) to IST at 6 months. However, for patients who initially responded to therapy, telomere length as a continuous variable inversely correlated with relapse rate (P=0.01). When telomere length was treated as a categorical variable, patients with shorter telomeres (below the 50th percentile of telomere distribution) had 2.5 times higher probability to relapse in five years then did patients with longer telomeres (P=0.002). Since telomere length physiologically shortens with aging, patients were stratified as being either greater than or equal/less than fifty years old to address whether shortened telomeres were a surrogate marker of age. In patients over 50 only (n=54), short telomere length but not age predicted relapse (P =0.04) in a multivariate model including telomere length, age, and pre-treatment blood counts, indicating that telomere length associated with relapse independently of age. Approximately 10% of patients undergoing IST eventually develop secondary clonal evolution. In this cohort 11/168 patients developed cytogenetic abnormalities, myelodysplastic syndrome, or leukemia in 5 years, and telomere length inversely correlated with risk for clonal evolution (P=0.01). These results indicate that leukocytes’ telomere length predicts sustained response to IST, possibly serving as a biological marker for HSC reserve. While initial response to treatment results from reducing cytotoxic T-cells, patients with short telomeres may quickly reach a critical length threshold where further stem cell division is not possible. Patients in this cohort with the shortest telomeres also were at risk for developing clonal evolution.

scholarly journals Telomere length in peripheral blood leukocytes and risk of renal cell carcinoma

2019 ◽  
Vol 8 (S4) ◽  
pp. S397-S403
Author(s):  
Jong Y. Park ◽  
Hung N. Luu ◽  
Hyun Y. Park ◽  
Hui-Yi Lin ◽  
Selina Radlein ◽  
...  
Keyword(s):  
Renal Cell Carcinoma ◽  
Cell Carcinoma ◽  
Telomere Length ◽  
Peripheral Blood ◽  
Renal Cell ◽  
Peripheral Blood Leukocytes ◽  
Blood Leukocytes

scholarly journals Comparison of the Relative Telomere Length Measured in Leukocytes and Eleven Different Human Tissues

2014 ◽  
pp. S343-S350 ◽  
Author(s):  
D. DLOUHA ◽  
J. MALUSKOVA ◽  
I. KRALOVA LESNA ◽  
V. LANSKA ◽  
J. A. HUBACEK
Keyword(s):  
Telomere Length ◽  
Peripheral Blood ◽  
Subcutaneous Fat ◽  
Relative Length ◽  
Biological Aging ◽  
Human Tissues ◽  
Peripheral Blood Leukocytes ◽  
Blood Leukocytes ◽  
Relative Telomere Length ◽  
Peripheral Leukocytes

The relative length of telomeres measured in peripheral blood leukocytes is a commonly used system marker for biological aging and can also be used as a biomarker of cardiovascular aging. However, to what extent the telomere length in peripheral leukocytes reflects telomere length in different organ tissues is still unclear. Therefore, we have measured relative telomere length (rTL) in twelve different human tissues (peripheral blood leukocytes, liver, kidney, heart, spleen, brain, skin, triceps, tongue mucosa, intercostal skeletal muscle, subcutaneous fat, and abdominal fat) from twelve cadavers (age range of 29 week of gestation to 88 years old). The highest rTL variability was observed in peripheral leukocytes, and the lowest variability was found in brain. We found a significant linear correlation between leukocyte rTL and both intercostal muscle (R=0.68, P<0.02) and liver rTL (R=0.60, P<0.05) only. High rTL variability was observed between different organs from one individual. Furthermore, we have shown that even slight DNA degradation (modeled by sonication of genomic DNA) leads to false rTL shortening. We conclude that the rTL in peripheral leukocytes is not strongly correlated with the rTL in different organs.

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