scholarly journals Epithelial Stem/Progenitor Cells in the Embryonic Mouse Submandibular Gland

2010 ◽  
pp. 90-106 ◽  
Author(s):  
Isabelle M.A. Lombaert ◽  
Matthew P. Hoffman
Keyword(s):  
Progenitor Cells ◽  
Submandibular Gland ◽  
Embryonic Mouse

Maintenance of neural stem-progenitor cells by the lysosomal biosynthesis regulators TFEB and TFE3 in the embryonic mouse telencephalon

Stem Cells ◽  
2021 ◽  
Vol 39 (7) ◽  
pp. 929-944
Author(s):  
Naoya Yuizumi ◽  
Yujin Harada ◽  
Takaaki Kuniya ◽  
Takehiko Sunabori ◽  
Masato Koike ◽  
...  
Keyword(s):  
Progenitor Cells ◽  
Embryonic Mouse ◽  
Neural Stem Progenitor Cells

scholarly journals Specification and maintenance of oligodendrocyte precursor cells from neural progenitor cells: involvement of microRNA-7a

2012 ◽  
Vol 23 (15) ◽  
pp. 2867-2877 ◽  
Author(s):  
Xianghui Zhao ◽  
Jiang Wu ◽  
Minhua Zheng ◽  
Fang Gao ◽  
Gong Ju
Keyword(s):  
Progenitor Cells ◽  
Cell Fate ◽  
Neural Progenitor Cells ◽  
Neural Progenitor ◽  
Precursor Cells ◽  
Specific Gene ◽  
Oligodendrocyte Precursor Cells ◽  
Embryonic Mouse ◽  
New Strategy ◽  
Oligodendrocyte Precursor

The generation of myelinating cells from multipotential neural stem cells in the CNS requires the initiation of specific gene expression programs in oligodendrocytes (OLs). We reasoned that microRNAs (miRNAs) could play an important role in this process by regulating genes crucial for OL development. Here we identified miR-7a as one of the highly enriched miRNAs in oligodendrocyte precursor cells (OPCs), overexpression of which in either neural progenitor cells (NPCs) or embryonic mouse cortex promoted the generation of OL lineage cells. Blocking the function of miR-7a in differentiating NPCs led to a reduction in OL number and an expansion of neuronal populations simultaneously. We also found that overexpression of this miRNA in purified OPC cultures promoted cell proliferation and inhibited further maturation. In addition, miR-7a might exert the effects just mentioned partially by directly repressing proneuronal differentiation factors including Pax6 and NeuroD4, or proOL genes involved in oligodendrocyte maturation. These results suggest that miRNA pathway is essential in determining cell fate commitment for OLs and thus providing a new strategy for modulating this process in OL loss diseases.

scholarly journals Sox9 regulates cell state and activity of embryonic mouse mammary progenitor cells

2018 ◽  
Vol 1 (1) ◽  
Author(s):  
Naoko Kogata ◽  
Philip Bland ◽  
Mandy Tsang ◽  
Erik Oliemuller ◽  
Anne Lowe ◽  
...  
Keyword(s):  
Progenitor Cells ◽  
Embryonic Mouse ◽  
Cell State

scholarly journals MON-727 The Chromatin Landscape of Glucocorticoid Regulated Genes in Mouse Embryonic Neural Stem / Progenitor Cells

2020 ◽  
Vol 4 (Supplement_1) ◽  
Author(s):  
Kimberly Berry ◽  
Uma Chandran ◽  
Fangping Mu ◽  
Donald DeFranco
Keyword(s):  
Progenitor Cells ◽  
Target Genes ◽  
Therapeutic Potential ◽  
Transcriptional Response ◽  
Clinical Intervention ◽  
Enrichment Analysis ◽  
Open Chromatin ◽  
Transcriptional Responses ◽  
Embryonic Mouse

Abstract Stem/Progenitor Cells Antenatal administration of Dexamethasone (Dex), a synthetic glucocorticoid (GC), is a common clinical intervention for women at risk for preterm birth or in preterm labor that effectively reduces fetal risk of mortality and bronchopulmonary-related comorbidities. Despite the therapeutic potential of Dex, excess GC act adversely in the developing central nervous system to reprogram distinct neural circuits in the brain by acting through the glucocorticoid receptor (GR). For example, prenatal exposure to excess GCs can impact neural stem and progenitor cell (NSPC) proliferation leading to long-term alterations in prefrontal cortical neuronal complexity, which could contribute to behavioral and cognitive impairments later in life. The GR is a member of the nuclear receptor superfamily that, when bound by a ligand, translocates from the cytoplasm to the nucleus and associates indirectly or directly with DNA elements (e.g. glucocorticoid responsive elements or GREs) resulting in the activation and/or repression of target genes. While GR-regulated transcriptomes have been identified in many NSPC models, the mechanisms responsible for programming these cells for GC-responsiveness remain largely unknown. We therefore used transposase accessible chromatin followed by genome-wide sequencing (Omni ATAC-seq) to characterize the chromatin landscape of primary embryonic mouse NSPCs in response to an acute in vitro treatment with Dex. We identified a small, yet distinct fraction (0.002%, p<0.05) of open chromatin sites that were Dex-inducible. 95% of these Dex-induced changes in chromatin accessibility occur within intronic or intergenic regions, suggesting the presence of long-range enhancer-promoter contacts that mediate NSPC transcriptional responses to Dex. Motif enrichment analysis revealed putative GRE sites located in Dex-inducible open chromatin within -5kb/+2kb of a Dex-induced gene, providing possible DNA targets of GR for further validation. A number of other transcription factors implicated in neurodevelopmental processes were found to underlie both Dex-inducible and constitutively open chromatin regions. Characterization of the precise epigenetic and transcriptional response to excess GC in-utero, and its influence on acute and chronic neurological outcomes, will encourage the development of alternative GC treatment regimens that could protect the developing brain from insult while providing optimal health outcomes in neonates.

scholarly journals Generation of functional salivary gland tissue from human submandibular gland stem/progenitor cells

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Yi Sui ◽  
Siqi Zhang ◽  
Yongliang Li ◽  
Xin Zhang ◽  
Waner Hu ◽  
...  
Keyword(s):  
Salivary Gland ◽  
Progenitor Cells ◽  
Submandibular Gland ◽  
Salivary Gland Tissue ◽  
Gland Tissue

scholarly journals Embryonic mouse lung epithelial progenitor cells co-express immunohistochemical markers of diverse mature cell lineages.

1996 ◽  
Vol 44 (2) ◽  
pp. 113-123 ◽  
Author(s):  
C W Wuenschell ◽  
M E Sunday ◽  
G Singh ◽  
P Minoo ◽  
H C Slavkin ◽  
...  
Keyword(s):  
Progenitor Cells ◽  
Cell Types ◽  
Neuroendocrine Cells ◽  
Developmental Expression ◽  
Mouse Lung ◽  
Marker Genes ◽  
Mature Cell ◽  
Alveolar Cells ◽  
Embryonic Mouse ◽  
Cell Lineages

Developmental expression of marker genes representative of different mature cell types can be used to study differentiation of cell lineages. We used immunohistochemistry to study expression in developing mouse lung of calcitonin gene-related peptide (CGRP), Clara cell 10-KD protein (CC10), and surfactant protein-A (SP-A), markers that are differentially expressed in neuroendocrine cells, Clara cells, and Type II alveolar cells. Two distinct developmental phases were revealed. The earlier phase (embryonic days 13-15; E13-E15) was characterized by CGRP, CC10, and SP-A immunostaining in all epithelial cells of the distal airways, with the three patterns being virtually identical in adjacent sections. The later phase (E16-E18) was characterized by emergence of staining of the differentiated cell types. These expression patterns were recapitulated in serumless organ culture, demonstrating that information necessary to generate both phases of gene expression is present within the lung analage by E11. We conclude that CGRP, CC10, and SP-A are co-expressed in most or all cells of the distal lung epithelium at E13-E15 and later become restricted to different cell lineages. This transient expression in progenitor cells of gene products characteristic of diverse differentiated cell types may reflect an underlying mechanism of gene regulation.

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