Regulation of Multilineage Gene Expression and Apoptosis during in vitro Expansion of Human Bone Marrow Stromal Cells with Different Cell Culture Media

2010 ◽  
Vol 192 (4) ◽  
pp. 211-220 ◽  
Author(s):  
Huiyong Zhu ◽  
Nicolai Miosge ◽  
Jutta Schulz ◽  
Henning Schliephake
Keyword(s):  
Gene Expression ◽  
Cell Culture ◽  
Bone Marrow ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Culture Media ◽  
Marrow Stromal Cells ◽  
Cell Culture Media ◽  
In Vitro Expansion

Evaluation of in vitro gene expression of GDNF in transfected bone marrow stromal cells

2011 ◽  
Vol 44 (13) ◽  
pp. S306-S307 ◽  
Author(s):  
Ali Nourizadeh ◽  
Tiraihi Taki ◽  
Seyed A. Mesbah-Namin ◽  
Masoumeh Rajabi
Keyword(s):  
Gene Expression ◽  
Bone Marrow ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Marrow Stromal Cells

Human bone marrow stromal cells: In vitro expansion and differentiation for bone engineering

Biomaterials ◽  
2006 ◽  
Vol 27 (36) ◽  
pp. 6150-6160 ◽  
Author(s):  
G. Ciapetti ◽  
L. Ambrosio ◽  
G. Marletta ◽  
N. Baldini ◽  
A. Giunti
Keyword(s):  
Bone Marrow ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Marrow Stromal Cells ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Bone Engineering ◽  
In Vitro Expansion

scholarly journals A Method for Measurement of Drug Sensitivity of Myeloma Cells Co-Cultured with Bone Marrow Stromal Cells

2013 ◽  
Vol 18 (6) ◽  
pp. 637-646 ◽  
Author(s):  
Kristine Misund ◽  
Katarzyna A. Baranowska ◽  
Toril Holien ◽  
Christoph Rampa ◽  
Dionne C. G. Klein ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Cell Survival ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Large Scale ◽  
Stromal Cell ◽  
Marrow Stromal Cells ◽  
Cell Nuclei ◽  
Myeloma Cells

The tumor microenvironment can profoundly affect tumor cell survival as well as alter antitumor drug activity. However, conventional anticancer drug screening typically is performed in the absence of stromal cells. Here, we analyzed survival of myeloma cells co-cultured with bone marrow stromal cells (BMSC) using an automated fluorescence microscope platform, ScanR. By staining the cell nuclei with DRAQ5, we could distinguish between BMSC and myeloma cells, based on their staining intensity and nuclear shape. Using the apoptotic marker YO-PRO-1, the effects of drug treatment on the viability of the myeloma cells in the presence of stromal cells could be measured. The method does not require cell staining before incubation with drugs, and less than 5000 cells are required per condition. The method can be used for large-scale screening of anticancer drugs on primary myeloma cells. This study shows the importance of stromal cell support for primary myeloma cell survival in vitro, as half of the cell samples had a marked increase in their viability when cultured in the presence of BMSC. Stromal cell–induced protection against common myeloma drugs is also observed with this method.

In vitro differentiation of human bone marrow stromal cells into neural precursor cells using small molecules

2021 ◽  
Vol 363 ◽  
pp. 109340
Author(s):  
Abeer Sallam ◽  
Thangirala Sudha ◽  
Noureldien H.E. Darwish ◽  
Samar Eghotny ◽  
Abeer E-Dief ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Small Molecules ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Precursor Cells ◽  
Marrow Stromal Cells ◽  
Neural Precursor Cells ◽  
Neural Precursor ◽  
In Vitro Differentiation
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