scholarly journals Prevention of Mast Cell Degranulation by Disodium Cromoglycate Delayed the Regression of Hypoxic Pulmonary Hypertension in Rats

Respiration ◽  
2010 ◽  
Vol 80 (4) ◽  
pp. 335-339 ◽  
Author(s):  
Hana Maxová ◽  
Marianna Vasiľková ◽  
Jana Novotná ◽  
Olga Vajnerová ◽  
Alena Baňsová ◽  
...  
Keyword(s):  
Pulmonary Hypertension ◽  
Mast Cell ◽  
Mast Cell Degranulation ◽  
Disodium Cromoglycate ◽  
Hypoxic Pulmonary Hypertension

Prevention of Mast Cell Degranulation by Disodium Cromoglycate Attenuates the Development of Hypoxic Pulmonary Hypertension in Rats Exposed to Chronic Hypoxia

Respiration ◽  
2008 ◽  
Vol 76 (1) ◽  
pp. 102-107 ◽  
Author(s):  
Alena Baňasová ◽  
Hana Maxová ◽  
Václav Hampl ◽  
Martin Vízek ◽  
Viera Povýšilová ◽  
...  
Keyword(s):  
Pulmonary Hypertension ◽  
Mast Cell ◽  
Chronic Hypoxia ◽  
Mast Cell Degranulation ◽  
Disodium Cromoglycate ◽  
Hypoxic Pulmonary Hypertension

scholarly journals Mast cells degranulation affects angiogenesis in the rat uterine cervix during pregnancy

Reproduction ◽  
2004 ◽  
Vol 127 (3) ◽  
pp. 379-387 ◽  
Author(s):  
J Varayoud ◽  
J G Ramos ◽  
V L Bosquiazzo ◽  
M Muñoz-de-Toro ◽  
E H Luque
Keyword(s):  
Mast Cell ◽  
Mast Cells ◽  
Blood Vessels ◽  
Uterine Cervix ◽  
Vascular System ◽  
Mast Cell Degranulation ◽  
Endothelial Cell Proliferation ◽  
Disodium Cromoglycate ◽  
Mast Cell Stabilizer ◽  
Vascular Area

During pregnancy, it is essential that sufficient nutrients are supplied by the vascular system to support the dramatic modifications of the rat uterine cervix. Angiogenesis refers to the growth of new blood vessels from pre-existing microcirculation and mast cells have been associated with this process. This study examined the modifications of the vascular compartment and the distribution of mast cells on cervical tissue during pregnancy. Using disodium cromoglycate as a mast cell stabilizer, we determined the effects of the mast cell degranulation on cervical angiogenesis. Mast cell distribution and their degranulation status were evaluated by immunohistochemistry. Endothelial cell proliferation was measured by bromodeoxyuridine incorporation. Vascular areas (absolute and relative) and maturation indices were assessed by quantitative immunohistochemistry of von Willebrand factor and α-smooth muscle actin respectively. Mast cells were predominantly observed during the first half of pregnancy in the perivascular zones. The values of bromodeoxyuridine incorporation, absolute vascular area and vascular maturation index exhibited a significant increase throughout pregnancy. All animals that received mast cell stabilizer showed more than 40% of non-degranulated mast cells. Treated rats exhibited a decrease in endothelial proliferation and in relative vascular area; in addition, a large proportion of mature blood vessels was observed, suggesting a diminished level of new vessel formation. The effects of the mast cell stabilizer were sustained beyond the end of treatment. This is the first report that brings evidence that mast cell degranulation could be a necessary process to contribute to the normal angiogenesis of the rat cervix during pregnancy. Further investigations are needed to elucidate the possible implications of abnormal vascular development of the uterine cervix on the physiological process of ripening and parturition.

Disodium cromoglycate stabilizes mast cell degranulation while reducing the number of hemoglobin-induced microvascular leaks in rat mesentery

2004 ◽  
Vol 286 (5) ◽  
pp. H1750-H1756 ◽  
Author(s):  
Maxwell I. Ginsburg ◽  
Ann L. Baldwin
Keyword(s):  
Mast Cell ◽  
Mast Cells ◽  
Blood Substitutes ◽  
Mast Cell Degranulation ◽  
Disodium Cromoglycate ◽  
Sprague Dawley ◽  
Plasma Albumin ◽  
Rat Mesentery ◽  
Sprague Dawley Rats ◽  
Infusion Experiment

Blood substitutes, such as diaspirin cross-linked Hb (DBBF-Hb), have been considered for use during blood transfusions. Unfortunately, bolus injection of modified Hb has been shown to rapidly increase the leakage of microvessels to plasma albumin. This effect may result from production of excess reactive oxygen species (ROS) and could be linked to the observed increase in degranulated mast cells (DMC). Disodium cromoglycate (cromolyn) stabilizes mast cells and therefore might minimize the venular permeability in the rat mesentery. In 10 anesthetized Sprague-Dawley rats, the mesenteric preparation was continuously suffused with cromolyn while the microvasculature was filled with DBBF-Hb solution (10 mg/ml) for 10 min. Six animals received cromolyn pretreatment [two intravascular injections over 30 min ( experiment A)] and four animals received pretreatment with 2% HEPES-buffered saline (HBS)-BSA ( experiment B). Two more animals were pretreated with HBS-BSA without DBBF-Hb infusion but with cromolyn suffusion ( experiment C). Another set of experiments was performed on five animals without cromolyn suffusion or any pretreatment but with DBBF-Hb infusion ( experiment D). All groups then received a 1-min perfusion of FITC-albumin, fixation for 60 min, and microscopic examination. Experiments A and B demonstrated a significant reduction in the number of venular leaks and DMC compared with experiment D, but not in the area of venular leaks. These results suggest mast cell degranulation is not a major contributor to microvascular leakage induced by DBBF-Hb.

Hypoxic pulmonary hypertension in the mast cell-deficient mouse

1983 ◽  
Vol 54 (3) ◽  
pp. 680-686 ◽  
Author(s):  
Y. J. Zhu ◽  
R. Kradin ◽  
R. D. Brandstetter ◽  
G. Staton ◽  
J. Moss ◽  
...  
Keyword(s):  
Pulmonary Hypertension ◽  
Mast Cell ◽  
Deficient Mouse ◽  
Hypoxic Pulmonary Hypertension
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