Human Chorionic Gonadotropin: Inhibitory Effect on Mixed Lymphocyte Cultures

1973 ◽  
Vol 4 (5-6) ◽  
pp. 263-269 ◽  
Author(s):  
F. Teasdale ◽  
E.W. Adcock, III ◽  
C.S. August ◽  
Sheila Cox ◽  
F.C. Battaglia ◽  
...  
Keyword(s):  
Chorionic Gonadotropin ◽  
Human Chorionic Gonadotropin ◽  
Inhibitory Effect ◽  
Lymphocyte Cultures ◽  
Mixed Lymphocyte Cultures

scholarly journals Selective modification of a private I-A allo-stimulating determinant(s) upon association of antigen with an antigen-presenting cell.

1984 ◽  
Vol 159 (4) ◽  
pp. 1238-1252 ◽  
Author(s):  
K L Rock ◽  
B Benacerraf
Keyword(s):  
T Cell ◽  
Interleukin 2 ◽  
Inhibitory Effect ◽  
Stimulator Cell ◽  
Antigen Presenting Cell ◽  
Lymphocyte Cultures ◽  
Nominal Antigen ◽  
Mixed Lymphocyte Cultures ◽  
Antigen Presenting

A large panel of alloreactive, interleukin 2 (IL-2)-producing T cell hybridomas was constructed from B10 alpha BALB/c primary mixed lymphocyte cultures (MLC). Functional hybrids had specificity for either I-Ad or I-Ed. These cells were used to probe determinants on Ia molecules in an attempt to detect molecular association between a nominal antigen and an Ia molecule on an antigen-presenting cell (APC). The response of a small number of these clones was significantly blocked by the addition of the Ir gene-controlled copolymer L-glutamic acid60-L-alanine30-L-tyrosine10 (GAT) to culture. A comparison of the inhibited and uninhibited hybrids revealed an identical dose response curve. Further, both types of hybrids were activated by the same stimulator cell and frequently recognized the identical Ia molecule on that cell. Nevertheless, the inhibitory effect of GAT was localized to the stimulator cell and not to the T cell hybrids. All of the hybrids whose stimulation was blocked had specificity for the I-A molecule, which is the gene product known to control and restrict responsiveness to GAT. Further, only GT, but not a number of other related antigens, was also specifically inhibitory, which correlates with the known associational specificity of these antigens on an APC. Finally, the same stimulator cell could be shown to coordinately lose an allostimulatory determinant(s), while it was gaining an I-Ad plus GAT determinant(s). The implications of these findings on the nature of antigen-Ia association and on the role of polymorphic Ia determinants are discussed.

scholarly journals Immunohistomorphometric Features of ACTH Cells in Juvenile Rats after Treatment with Estradiol or Human Chorionic Gonadotropin

2012 ◽  
Vol 31 (1) ◽  
pp. 34-39 ◽  
Author(s):  
Verica Milošević ◽  
Danijela Todorović ◽  
Miroslava Veličković ◽  
Nataša Ristić ◽  
Gordana Ušćebrka ◽  
...  
Keyword(s):  
Chorionic Gonadotropin ◽  
Human Chorionic Gonadotropin ◽  
Neonatal Period ◽  
Secretory Activity ◽  
Treated Group ◽  
Inhibitory Effect ◽  
Volume Density ◽  
Female Rats ◽  
Female Rat ◽  
Juvenile Rats

Immunohistomorphometric Features of ACTH Cells in Juvenile Rats after Treatment with Estradiol or Human Chorionic GonadotropinEstradiol and human chorionic gonadotropin (hCG) are very important in controlling the secretory activity of hormone producing cells in the female rat pituitary glands. The aim of the present study was to examine the morphometric parameters of immunohistochemically labeled ACTH cells in juvenile (16th day) female rat pituitaries after treatment with five doses of estradiol dipropionate (EDP) and two doses of hCG during the neonatal period of life. The controls were treated on the same schedule with an equivalent volume of vehicle. All animals were sacrificed 24 h after the last treatment. ACTH-producing cells were studied using the peroxidase-antiperoxidase immunohistochemical procedure. The absolute and relative pituitary weights were increased (p<0.05) only in the EDP treated group by 120.0% and by 121.1% respectively, in comparison with the controls. In this group, the volume of ACTH cells, volume of their nuclei and volume density were significantly decreased (p<0.05) by 6.4%, 33.3% and 46.2% respectively, compared to the corresponding controls. After treatment with hCG, there were no significant (p>0.05) changes neither in the volume of ACTH cells nor in the volume of their nuclei, in comparison with the controls. On the basis of the results obtained in our study, it can be concluded that EDP, injected into female rats during the neonatal period of life, has an inhibitory effect on the immunohistomorphometric parameters of ACTH cells, but such an effect is not clearly expressed after treatment with hCG.

Effect of Tokishakuyakusan on Corpus Luteum Endothelin

1991 ◽  
Vol 19 (03n04) ◽  
pp. 251-258 ◽  
Author(s):  
Satoshi Usuki
Keyword(s):  
Corpus Luteum ◽  
Chorionic Gonadotropin ◽  
Human Chorionic Gonadotropin ◽  
Inhibitory Effect ◽  
Corpora Lutea ◽  
High Concentration ◽  
Endothelin 1 ◽  
Serum Gonadotropin ◽  
Wet Weight

The presence of endothelin-1 (ET) and effect of Tokishakuyakusan (TS) on ET in rat corpora lutea (CL) was investigated in superovulated ovaries, induced with pregnant mare's serum gonadotropin and human chorionic gonadotropin. A high concentration of ET was found in the CL. The level of ET was significantly lower in the CL from TS-treated rats than that in TS-untreated rats (402.68 versus 575.60 pg/g wet weight, p < 0.05). In contrast, the ET levels in plasma were by far lower than those in CL. These data indicate an inhibitory effect of TS on ET, an intraovarian peptide, production or accumulation in the CL.

scholarly journals Urocortin 1 Inhibits Rat Leydig Cell Function

Endocrinology ◽  
2008 ◽  
Vol 149 (12) ◽  
pp. 6425-6432 ◽  
Author(s):  
Catherine L. Rivier
Keyword(s):  
Chorionic Gonadotropin ◽  
Leydig Cell ◽  
Human Chorionic Gonadotropin ◽  
Leydig Cells ◽  
Cell Function ◽  
Cell Activity ◽  
Inhibitory Effect ◽  
Urocortin 1 ◽  
Stress Models

Corticotropin-releasing factor (CRF) has previously been reported in rat testes in which it inhibits Leydig cells activity. However, recent studies in our laboratory have suggested that some of the effects originally attributed to CRF were instead due to the related peptide Urocortin 1 (Ucn 1) and that this latter hormone, not CRF, was detectable in Leydig cells. We show here that Ucn 1 [a mixed CRF receptor (CRFR) type 1 and CRFR2 agonist] and the CRFR1-selective peptide Stressin 1, but not Ucn 2 or Ucn 3 (both considered selective CRFR2 ligands), significantly blunt the testosterone response to human chorionic gonadotropin. The effect of Ucn 1 is observed regardless of whether this peptide is injected iv or directly into the testes, and it is reversed by the mixed CRFR1/R2 antagonist Astressin B. Blockade of GnRH receptors with the antagonist Azalin B does not interfere with the influence of Ucn 1, thereby demonstrating that pituitary luteinizing hormone does not appear to be involved in this model. Collectively these results suggest that Ucn 1, not CRF, is present in the rat testes and interferes with Leydig cell activity. However, whereas we previously reported that alcohol up-regulated gonadal Ucn 1 gene expression, CRF receptor antagonists were unable to reverse the inhibitory effect exerted by alcohol on human chorionic gonadotropin-induced testosterone release. The functional role played by testicular Ucn 1 in stress models characterized by blunted androgen levels therefore needs to be further investigated.

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