Constitutive and Tumor Necrosis Factor-Alpha-Stimulated Activation of Nuclear Factor-KappaB in Immortalized Endometriotic Cells and Their Suppression by Trichostatin A

2010 ◽  
Vol 70 (1) ◽  
pp. 23-33 ◽  
Author(s):  
Yan Wu ◽  
Anna Starzinski-Powitz ◽  
Sun-Wei Guo
Keyword(s):  
Tumor Necrosis Factor ◽  
Tumor Necrosis Factor Alpha ◽  
Tumor Necrosis ◽  
Trichostatin A ◽  
Nuclear Factor Kappab ◽  
Nuclear Factor ◽  
Necrosis Factor Alpha ◽  
Factor Alpha ◽  
Necrosis Factor

scholarly journals Tumor Necrosis Factor Alpha-Mediated Reduction of KLF2 Is Due to Inhibition of MEF2 by NF-κB and Histone Deacetylases

2005 ◽  
Vol 25 (14) ◽  
pp. 5893-5903 ◽  
Author(s):  
Ajay Kumar ◽  
Zhiyong Lin ◽  
Sucharita SenBanerjee ◽  
Mukesh K. Jain
Keyword(s):  
Tumor Necrosis Factor ◽  
Tumor Necrosis Factor Alpha ◽  
Histone Deacetylases ◽  
Tumor Necrosis ◽  
Trichostatin A ◽  
Cellular Systems ◽  
Necrosis Factor Alpha ◽  
Tnf Α ◽  
Factor Alpha ◽  
Necrosis Factor

ABSTRACT Activation of the endothelium by inflammatory cytokines is a key event in the pathogenesis of vascular disease states. Proinflammatory cytokines repress the expression of KLF2, a recently identified transcriptional inhibitor of the cytokine-mediated activation of endothelial cells. In this study the molecular basis for the cytokine-mediated inhibition of KLF2 is elucidated. Tumor necrosis factor alpha (TNF-α) potently inhibited KLF2 expression. This effect was completely abrogated by a constitutively active form of IκBα, as well as treatment with trichostatin A, implicating a role for the NF-κB pathway and histone deacetylases. Overexpression studies coupled with observations with p50/p65 null cells support an essential role for p65. A combination of promoter deletion and mutational analyses, chromatin immunoprecipitation assays, and coimmunoprecipitation studies indicates that p65 and histone deacetylases 4 cooperate to inhibit the ability of MEF2 factors to induce the KLF2 promoter. These studies identify a novel mechanism by which TNF-α can inhibit endothelial gene expression. Furthermore, the inhibition of MEF2 function by p65 and HDAC4 has implications for other cellular systems where these factors are operative.

scholarly journals Tumor necrosis factor alpha-mediated inhibition of melanogenesis is dependent on nuclear factor kappa B activation

Oncogene ◽  
1999 ◽  
Vol 18 (8) ◽  
pp. 1553-1559 ◽  
Author(s):  
Walter Englaro ◽  
Philippe Bahadoran ◽  
Corine Bertolotto ◽  
Roser Buscà ◽  
Benoit Dérijard ◽  
...  
Keyword(s):  
Tumor Necrosis Factor ◽  
Tumor Necrosis Factor Alpha ◽  
Tumor Necrosis ◽  
Nuclear Factor Kappa B ◽  
Nuclear Factor ◽  
Necrosis Factor Alpha ◽  
Nuclear Factor Kappa ◽  
Factor Alpha ◽  
Necrosis Factor
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