The Griffoniasimplicifolia I Isolectin B4 and Tomato Lectin Recognize Astrocytes instead of Microglia in Adult Hamster Retina

2008 ◽  
Vol 5 (4) ◽  
pp. 166-181
Author(s):  
Kwok-hang Chung ◽  
Eric Y.P. Cho
Keyword(s):  
Tomato Lectin ◽  
Adult Hamster ◽  
Isolectin B4

Oligodendrocytes in Developing Hameter Cerebral Cortex

1976 ◽  
Vol 34 ◽  
pp. 126-127
Author(s):  
MB. Tank Buschmann
Keyword(s):  
Cerebral Cortex ◽  
Optic Nerve ◽  
Frontal Cortex ◽  
Osmium Tetroxide ◽  
Sodium Cacodylate Buffer ◽  
Sodium Cacodylate ◽  
Tissue Samples ◽  
Cacodylate Buffer ◽  
Layer V ◽  
Adult Hamster

Development of oligodendrocytes in rat corpus callosum was described as a sequential change in cytoplasmic density which progressed from light to medium to dark (1). In rat optic nerve, changes in cytoplasmic density were not observed, but significant changes in morphology occurred just prior to and during myelination (2). In our study, the ultrastructural development of oligodendrocytes was studied in newborn, 5-, 10-, 15-, 20-day and adult frontal cortex of the golden hamster (Mesocricetus auratus).Young and adult hamster brains were perfused with paraformaldehyde-glutaraldehyde in sodium cacodylate buffer at pH 7.3 according to the method of Peters (3). Tissue samples of layer V of the frontal cortex were post-fixed in 2% osmium tetroxide, dehydrated in acetone and embedded in Epon-Araldite resin.

Monoclonal Antibodies against the Tn-Specific Isolectin B4 fromVicia villosaSeeds: Characterization of the Epitope of the Blocking Antibody VV34

2004 ◽  
Vol 23 (1) ◽  
pp. 39-44 ◽  
Author(s):  
Andrea Medeiros ◽  
Nora Berois ◽  
Henia Balter ◽  
Ana Robles ◽  
Enrique Perez-Payá ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Blocking Antibody ◽  
Isolectin B4

Cloning of two adult hamster globin cDNAs (α and βmajor)

1992 ◽  
Vol 1130 (3) ◽  
pp. 343-344 ◽  
Author(s):  
Kai M. Lee ◽  
Milayna Subar ◽  
Hong Li ◽  
Thalia Boussios
Keyword(s):  
Adult Hamster

scholarly journals The effect of spinal cord injury on the neurochemical properties of vagal sensory neurons

2015 ◽  
Vol 308 (12) ◽  
pp. R1021-R1033 ◽  
Author(s):  
April N. Herrity ◽  
Jeffrey C. Petruska ◽  
David P. Stirling ◽  
Kristofer K. Rau ◽  
Charles H. Hubscher
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Vagus Nerve ◽  
Sensory Neurons ◽  
Receptor Expression ◽  
Vagal Afferents ◽  
Cord Injury ◽  
Male Wistar Rats ◽  
Neurochemical Plasticity ◽  
Isolectin B4

The vagus nerve is composed primarily of nonmyelinated sensory neurons whose cell bodies are located in the nodose ganglion (NG). The vagus has widespread projections that supply most visceral organs, including the bladder. Because of its nonspinal route, the vagus nerve itself is not directly damaged from spinal cord injury (SCI). Because most viscera, including bladder, are dually innervated by spinal and vagal sensory neurons, an impact of SCI on the sensory component of vagal circuitry may contribute to post-SCI visceral pathologies. To determine whether SCI, in male Wistar rats, might impact neurochemical characteristics of NG neurons, immunohistochemical assessments were performed for P2X3 receptor expression, isolectin B4 (IB4) binding, and substance P expression, three known injury-responsive markers in sensory neuronal subpopulations. In addition to examining the overall population of NG neurons, those innervating the urinary bladder also were assessed separately. All three of the molecular markers were represented in the NG from noninjured animals, with the majority of the neurons binding IB4. In the chronically injured rats, there was a significant increase in the number of NG neurons expressing P2X3 and a significant decrease in the number binding IB4 compared with noninjured animals, a finding that held true also for the bladder-innervating population. Overall, these results indicate that vagal afferents, including those innervating the bladder, display neurochemical plasticity post-SCI that may have implications for visceral homeostatic mechanisms and nociceptive signaling.

Tomato Lectin

2008 ◽  
pp. 165-192
Author(s):  
Willy J. Peumans ◽  
Els J.M. Van Damme
Keyword(s):  
Tomato Lectin

scholarly journals Variants of Oxidized Regenerated Cellulose and Their Distinct Effects on Neuronal Tissue

2021 ◽  
Vol 22 (21) ◽  
pp. 11467
Author(s):  
Joshua Kleine ◽  
Sandra Leisz ◽  
Chalid Ghadban ◽  
Tim Hohmann ◽  
Julian Prell ◽  
...  
Keyword(s):  
Hippocampal Slice ◽  
Cellular Damage ◽  
Regenerated Cellulose ◽  
Clinical Settings ◽  
Oxidized Regenerated Cellulose ◽  
Tissue Properties ◽  
Neuronal Tissue ◽  
Fluorescence Dye ◽  
Isolectin B4 ◽  
Hematoxylin Eosin

Based on oxidized regenerated cellulose (ORC), several hemostyptic materials, such as Tabotamp®, Equicel® and Equitamp®, have been developed to approach challenging hemostasis in neurosurgery. The present study compares ORC that differ in terms of compositions and properties, regarding their structure, solubility, pH values and effects on neuronal tissue. Cytotoxicity was detected via DNA-binding fluorescence dye in Schwann cells, astrocytes, and neuronal cells. Additionally, organotypic hippocampal slice cultures (OHSC) were analyzed, using propidium iodide, hematoxylin-eosin, and isolectin B4 staining to investigate the cellular damage, cytoarchitecture, and microglia activation. Whereas Equicel® led to a neutral pH, Tabotamp® (pH 2.8) and Equitamp® (pH 4.8) caused a significant reduction of pH (p < 0.001). Equicel® and Tabotamp® increased cytotoxicity significantly in several cell lines (p < 0.01). On OHSC, Tabotamp® and Equicel® led to a stronger and deeper damage to the neuronal tissue than Equitamp® or gauze (p < 0.01). Equicel® increased strongly the number of microglia cells after 24 h (p < 0.001). Microglia cells were not detectable after Tabotamp® treatment, presumably due to an artifact caused by strong pH reduction. In summary, our data imply the use of Equicel®, Tabotamp® or Equitamp® for specific applications in distinct clinical settings depending on their localization or tissue properties.

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