Identification of Proliferating Keratinocytes in Middle Ear Cholesteatoma Using the Monoclonal Antibody Ki-67

ORL ◽  
1996 ◽  
Vol 58 (1) ◽  
pp. 23-26 ◽  
Author(s):  
Jesús Bujía ◽  
Anja Holly ◽  
Holger Sudhoff ◽  
Francisco Antolí-Candela ◽  
Mario Guzman Tapia ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Middle Ear ◽  
Ki 67 ◽  
Middle Ear Cholesteatoma

Role of Langerhans cells, Ki-67 protein and apoptosis in acquired cholesteatoma: prospective clinical study

2013 ◽  
Vol 127 (3) ◽  
pp. 252-259 ◽  
Author(s):  
V Akdogan ◽  
I Yilmaz ◽  
T Canpolat ◽  
L N Ozluoglu
Keyword(s):  
Middle Ear ◽  
Langerhans Cells ◽  
Prospective Clinical Study ◽  
Ear Canal ◽  
Ki 67 ◽  
Middle Ear Cholesteatoma ◽  
Proliferation And Apoptosis ◽  
Acquired Cholesteatoma ◽  
Open Versus

AbstractObjective:To investigate the role of Langerhans cells in the pathogenesis and clinical picture of middle-ear cholesteatoma.Subjects and methods:The study included 40 patients operated upon for a diagnosis of chronic otitis due to acquired cholesteatoma.Results and analysis:A closed surgical technique was used in 20 per cent of patients and an open technique in 80 per cent. Langerhans cells were more densely accumulated in cholesteatoma epithelium, compared with external ear canal skin (p < 0.001). Staining for Ki-67 protein was greater in cholesteatoma epithelium (p < 0.001) and Apo2.7 protein staining (indicating apoptosis) was more prominent (p < 0.001), compared with ear canal skin. Regarding significant relationships between clinical and pathological findings, staining for Ki-67 (p = 0.046) and Apo2.7 (p = 0.037) was more prominent in patients undergoing open versus closed surgery.Conclusion:Using cell proliferation and apoptosis markers, a dense Langerhans cell infiltration was found to occur as a host response to middle-ear cholesteatoma.

Expression Patterns of Ki-67 and Telomerase Activity in Middle Ear Cholesteatoma

2007 ◽  
Vol 28 (2) ◽  
pp. 204-207 ◽  
Author(s):  
Jerzy Kuczkowski ◽  
Tadeusz Pawelczyk ◽  
Alicja Bakowska ◽  
Waldemar Narozny ◽  
Boguslaw Mikaszewski
Keyword(s):  
Middle Ear ◽  
Expression Patterns ◽  
Telomerase Activity ◽  
Ki 67 ◽  
Middle Ear Cholesteatoma

scholarly journals The significance of the expression of cell proliferation and inflammation markers in the development of acquired middle ear cholesteatoma

2018 ◽  
Vol 75 (5) ◽  
pp. 487-495
Author(s):  
Milan Erdoglija ◽  
Ugljesa Grgurevic ◽  
Snezana Cerovic ◽  
Milena Jovic ◽  
Nenad Baletic ◽  
...  
Keyword(s):  
Middle Ear ◽  
External Auditory Canal ◽  
Clinical Picture ◽  
Clinical Characteristics ◽  
Biological Behavior ◽  
Ki 67 ◽  
Middle Ear Cholesteatoma ◽  
Significant Difference ◽  
Acquired Cholesteatoma ◽  
Cox 2

Background/Aim. Permanent proliferation and periodical infection are the main clinical characteristics of acquired middle ear cholesteatoma. The aim of this study was to research immunohistochemical characteristics of the skin along with the cholesteatoma process in the nearby tissue. This research should influence further studying of etiology and development of acquired middle ear cholesteatoma. Methods. We investigated clinical, histological and immunohistochemical characteristics of cholesteatoma in 50 samples from operated patients with acquired middle ear cholesteatomas. We classified all samples according to their clinical characteristics of cholesteatoma such as bone destruction, presence of infection or cholesteatoma extension and histological characteristics of cholesteatoma such as keratinisation, inflammatory process and extracellular matrix proliferation. We used mouse monoclonal antibodies for proliferating cell nuclear antigen (MAbs for PCNA), Ki-67, COX-2, CD 4 and CD 8 lymphocytes to investigate the expression of those characteristics in the cholesteatoma and in the control skin tissue. Statistical analyses were performed using SPSS for Windows version 16.0 (SPSS, Chicago, IL, USA). We used the independent group t-test, Spearman?s correlation analysis and Mann-Whitney U test to analyze statistical analysis. Results. Expression of PCNA, Ki-67, COX-2 and CD 8 lymphocytes in more serious clinical picture of cholesteatoma was almost equal as in less serious clinical picture of cholesteatoma. There was statistically significantly higher concentration of inflammation marker CD 4 lymphocytes, both in the acquired cholesteatoma and in the skin of bony portion of the external auditory canal near fibrocartilaginous annulus in more serious clinical picture of cholesteatoma than in less serious clinical picture of cholesteatoma (p < 0.01). There was statistically significant difference of expression of PCNA, Ki- 67, COX-2, CD 4 and CD 8 lymphocytes between all cholesteatoma samples and the skin of bony portion of the external auditory canal (p < 0.05) and statistically significant difference of expression of those markers between the skin of bony portion of the external auditory canal and retroauricular skin (p < 0.05). Conclusion. Inflammation of the skin of bony portion of the external auditory canal is a milestone in pathogenesis of acquired middle ear cholesteatoma. Expression of CD 4 lymphocytes can be the prognostic factor for acquired cholesteatoma clinical picture development. We found so much diversity in biological behavior through very different levels of cholesteatoma development. Expression of Ki-67 in acquired middle ear cholesteatoma is a reliable and stable marker of proliferation for acquired middle ear cholesteatoma.

Nucleoplasm staining patterns and cell cycle–associated expression of Ki-67 in middle ear cholesteatoma

2005 ◽  
Vol 26 (5) ◽  
pp. 296-301 ◽  
Author(s):  
Alexander M. Raynov ◽  
Sung-Kyun Moon ◽  
Yun-Hoon Choung ◽  
Sung Pyo Hong ◽  
Keehyun Park
Keyword(s):  
Cell Cycle ◽  
Middle Ear ◽  
Ki 67 ◽  
Middle Ear Cholesteatoma

A comparison of high resolution CT scan of temporal bone and operative findings in middle ear cholesteatoma

1993 ◽  
Vol 29 (5) ◽  
pp. 896
Author(s):  
Tae Beom Kweon ◽  
Hun Seong ◽  
Mal Soon Cheon ◽  
Hack Jin Kim ◽  
Keung Jae Jang ◽  
...  
Keyword(s):  
High Resolution ◽  
Ct Scan ◽  
Temporal Bone ◽  
Middle Ear ◽  
High Resolution Ct ◽  
Middle Ear Cholesteatoma ◽  
Operative Findings

Cell cycle-dependent reactivity with the monoclonal antibody Ki-67 during myeloid cell differentiation

1988 ◽  
Vol 179 (1) ◽  
pp. 79-88 ◽  
Author(s):  
Robert P. Wersto ◽  
Fritz Herz ◽  
Robert E. Gallagher ◽  
Leopold G. Koss
Keyword(s):  
Cell Cycle ◽  
Monoclonal Antibody ◽  
Cell Differentiation ◽  
Myeloid Cell ◽  
Ki 67 ◽  
Cycle Dependent

MRI of middle ear cholesteatoma: The importance of observer reliance from diffusion sequences

2021 ◽  
Author(s):  
Natalie Fischer ◽  
Michaela Plaikner ◽  
Volker H. Schartinger ◽  
Christian Kremser ◽  
Herbert Riechelmann ◽  
...  
Keyword(s):  
Middle Ear ◽  
Middle Ear Cholesteatoma

Pediatric middle ear cholesteatoma: the comparative study of congenital cholesteatoma and acquired cholesteatoma

2015 ◽  
Vol 273 (5) ◽  
pp. 1155-1160 ◽  
Author(s):  
Yuka Morita ◽  
Yutaka Yamamoto ◽  
Shinsuke Oshima ◽  
Kuniyuki Takahashi ◽  
Sugata Takahashi
Keyword(s):  
Comparative Study ◽  
Middle Ear ◽  
Congenital Cholesteatoma ◽  
Middle Ear Cholesteatoma ◽  
Acquired Cholesteatoma ◽  
The Comparative Study
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