Exogenous Heparin, Topically Administered, Aids the Remodelling of Connective Tissue in the Healing of Experimental Tympanic Membrane Perforations

ORL ◽  
1994 ◽  
Vol 56 (1) ◽  
pp. 45-50 ◽  
Author(s):  
Sten Hellström ◽  
Odd Spandow
Keyword(s):  
Connective Tissue ◽  
Tympanic Membrane ◽  
Tympanic Membrane Perforations

scholarly journals Effect of Basic Fibroblast Growth Factor on Perforated Chinchilla Tympanic Membranes

1997 ◽  
Vol 76 (8) ◽  
pp. 559-564 ◽  
Author(s):  
Norman R. Friedman ◽  
Charles G. Wright ◽  
Karen S. Pawlowski ◽  
William L. Meyerhoff
Keyword(s):  
Growth Factor ◽  
Connective Tissue ◽  
Tympanic Membrane ◽  
Fibroblast Growth Factor ◽  
Basic Fibroblast Growth Factor ◽  
Healing Process ◽  
Fibroblast Growth ◽  
Short Term ◽  
Tympanic Membrane Perforations ◽  
Perforated Tympanic Membrane

Basic fibroblast growth factor (bFGF) is a polypeptide mitogen which stimulates proliferation of epidermal and connective tissue cells. When applied to tympanic membrane perforations it has been reported to enhance healing and produce connective tissue hyperplasia. Previous work with animal models has shown that hyperplastic alterations of the tympanic membrane play an essential role in cholesteatoma development. This study was designed to further investigate the hyperplastic effects of bFGF and to determine if it might induce cholesteatoma formation during the healing process. Ten chinchillas received bilateral tympanic membrane perforations. In each animal, three doses of bFGF (400 nanograms per dose) were applied to the perforated tympanic membrane on one side; the opposite (control) ear received saline alone. The animals were terminated at either two or four weeks and studied histologically. Although the dosage and administration schedule used were consistent with previous studies utilizing other rodent species, there was little evidence that bFGF affected tympanic membrane healing in chinchillas. In both control and bFGF-treated ears, dense connective tissue occupied the lamina propria of the tympanic membrane, providing an effective barrier against ingrowth of skin toward the middle ear. No cholesteatomas developed in any animals included in the study. The results of this work indicate that the risk of cholesteatoma formation following administration of bFGF is minimal when it is applied short-term to acute perforations.

Animal Model for Persistent Tympanic Membrane Perforations

1993 ◽  
Vol 102 (6) ◽  
pp. 467-472 ◽  
Author(s):  
Odd Spandow ◽  
Sten Hellström
Keyword(s):  
Animal Model ◽  
Connective Tissue ◽  
Light Microscopy ◽  
Tympanic Membrane ◽  
Healing Rate ◽  
Epidermal Layer ◽  
Tissue Layer ◽  
Connective Tissue Layer ◽  
Once Daily ◽  
Tympanic Membrane Perforations

Topically applied hydrocortisone was used to develop an animal model for persistent tympanic membrane (TM) perforations. Hydrocortisone suspension was applied on the margins of TM perforations of standardized size in rats once daily for 10 days. The healing patterns of the TMs were mapped weekly and, when the perforations were about to close, daily. After 50 days, all hydrocortisone-treated perforations were open, whereas the controls closed within 9 to 12 days. At 3 months, when one third of the perforations still were open, the TMs were studied by otomicroscopy and light microscopy. All TMs were thickened and covered by keratin and wax. The thickened epidermal layer at the border of the TM perforations that remained open also draped the surface of the perforation facing the middle ear cavity. The thickened connective tissue layer contained abundant fibroblasts with their axes of length oriented at random. Both application of 1.4% hyaluronan and wounding of the perforation border enhanced the healing rate of the hydrocortisone-induced chronic TM perforation.

Correlation between tympanometry volume and three-dimensional computed tomography mastoid volumetry in tympanoplasty candidates

2021 ◽  
pp. 1-5
Author(s):  
L Epprecht ◽  
L Qingsong ◽  
N Stenz ◽  
S Hashimi ◽  
T Linder
Keyword(s):  
Computed Tomography ◽  
Tympanic Membrane ◽  
Cell Volume ◽  
Radiation Injury ◽  
Three Dimensional ◽  
Non Invasive ◽  
Tympanic Membrane Perforations ◽  
Computed Tomography Scans ◽  
Air Cells ◽  
Mastoid Air Cells

Abstract Objective Ventilation of the middle ear and mastoid air cells is believed to play an important role in the pathogenesis of chronic ear disease. Traditionally, ventilation is assessed by computed tomography. However, this exposes patients to cumulative radiation injury. In cases with a perforation in the tympanic membrane, tympanometry potentially presents a non-invasive alternative to measure the ventilated middle-ear and mastoid air cell volume. This study hypothesised that total tympanometry volume correlates with ventilated middle-ear and mastoid air cell volume. Method Total tympanometry volume was compared with ventilated middle-ear and mastoid air cell volume on computed tomography scans in 20 tympanic membrane perforations. Results There was a high correlation between tympanometry and computed tomography volumes (r = 0.78; p < 0.001). A tympanometry volume more than 2 ml predicted good ventilation on computed tomography. Conclusion These results may help reduce the need for pre-operative computed tomography in uncomplicated cases with tympanic membrane perforations.

scholarly journals Comparative study between fat plug and inlay butterfly cartilage grafts for myringoplasty in adults

2021 ◽  
Vol 37 (1) ◽  
Author(s):  
Ahmed Gamal Khafagy ◽  
Mohamed El-Begermy ◽  
Marwa Mohamed El-Begermy ◽  
Pretty O. Afifi
Keyword(s):  
Tympanic Membrane ◽  
Chronic Otitis Media ◽  
Fat Graft ◽  
Cartilage Graft ◽  
Tympanic Membrane Perforations ◽  
Number Of Patients ◽  
Significant Difference ◽  
Hearing Outcomes ◽  
Age Range

Abstract Background This study aims to compare the graft uptake rate and hearing improvement of fat graft versus inlay butterfly tragal cartilage in the repair of perforations in chronic otitis media mucosal in adults. In this retrospective study, twenty-eight patients were included with small dry anteroinferior tympanic membrane perforations (less than 1/3 of the tympanic membrane). The age range was 18 to 44 years old. Myringoplasty was done under general anesthesia for 8 patients with a fat graft (FG) and 20 patients with inlay butterfly cartilage graft (IBCG). Six months postoperatively, a follow-up evaluation was done for successful graft uptake and hearing outcomes. Results The success rate of graft uptake in the first group (fat graft) was 6/8 cases (75%) while in the second group (IBCG) was 19/20 (95%) with no statistically significant difference (P = 0.0148). Also, there was no statistical difference between the two groups as regards postoperative ABG, improvement changes in ABG, and number of patients with improved hearing. Conclusions Inlay butterfly cartilage graft is a useful graft in repairing small tympanic membrane perforations as regard graft take and hearing outcomes.

Autologous Serum Eardrops Therapy With a Chitin Membrane for Closing Tympanic Membrane Perforations

2008 ◽  
Vol 29 (6) ◽  
pp. 791-795 ◽  
Author(s):  
Seiji Kakehata ◽  
Yuki Hirose ◽  
Rei Kitani ◽  
Kazunori Futai ◽  
Shin-ichiro Maruya ◽  
...  
Keyword(s):  
Tympanic Membrane ◽  
Autologous Serum ◽  
Tympanic Membrane Perforations

scholarly journals Tympanic Membrane Perforations Closed with Fibrin Glue.

1993 ◽  
Vol 86 (5) ◽  
pp. 653-662 ◽  
Author(s):  
Michio Isono ◽  
Yasuhiko Fukuyama ◽  
Kiyotaka Murata
Keyword(s):  
Tympanic Membrane ◽  
Fibrin Glue ◽  
Tympanic Membrane Perforations
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