scholarly journals Directional Cell Migration and Chemotaxis in Wound Healing Response to PDGF-AA are Coordinated by the Primary Cilium in Fibroblasts

2010 ◽  
Vol 25 (2-3) ◽  
pp. 279-292 ◽  
Author(s):  
Linda Schneider ◽  
Michael Cammer ◽  
Jonathan Lehman ◽  
Sonja Nielsen ◽  
Charles Guerra ◽  
...  
Keyword(s):  
Wound Healing ◽  
Cell Migration ◽  
Primary Cilium ◽  
Healing Response ◽  
Wound Healing Response ◽  
Directional Cell Migration

scholarly journals Autologous micrograft accelerates endogenous wound healing response through ERK-induced cell migration

2019 ◽  
Vol 27 (5) ◽  
pp. 1520-1538 ◽  
Author(s):  
Martina Balli ◽  
Francesca Vitali ◽  
Adrian Janiszewski ◽  
Ellen Caluwé ◽  
Alvaro Cortés-Calabuig ◽  
...  
Keyword(s):  
Wound Healing ◽  
Cell Migration ◽  
Molecular Mechanism ◽  
Healing Process ◽  
Skin Lesions ◽  
Wound Healing Process ◽  
Transcriptional Signature ◽  
Beneficial Effects ◽  
Healing Response ◽  
Wound Healing Response

Abstract Defective cell migration causes delayed wound healing (WH) and chronic skin lesions. Autologous micrograft (AMG) therapies have recently emerged as a new effective and affordable treatment able to improve wound healing capacity. However, the precise molecular mechanism through which AMG exhibits its beneficial effects remains unrevealed. Herein we show that AMG improves skin re-epithelialization by accelerating the migration of fibroblasts and keratinocytes. More specifically, AMG-treated wounds showed improvement of indispensable events associated with successful wound healing such as granulation tissue formation, organized collagen content, and newly formed blood vessels. We demonstrate that AMG is enriched with a pool of WH-associated growth factors that may provide the starting signal for a faster endogenous wound healing response. This work links the increased cell migration rate to the activation of the extracellular signal-regulated kinase (ERK) signaling pathway, which is followed by an increase in matrix metalloproteinase expression and their extracellular enzymatic activity. Overall we reveal the AMG-mediated wound healing transcriptional signature and shed light on the AMG molecular mechanism supporting its potential to trigger a highly improved wound healing process. In this way, we present a framework for future improvements in AMG therapy for skin tissue regeneration applications.

scholarly journals Autologous micrograft accelerates endogenous wound healing response through ERK-induced cell migration

2019 ◽  
Author(s):  
Martina Balli ◽  
Francesca Vitali ◽  
Adrian Janiszewski ◽  
Ellen Caluwé ◽  
Alvaro Cortés-Calabuig ◽  
...  
Keyword(s):  
Wound Healing ◽  
Cell Migration ◽  
Molecular Mechanisms ◽  
Healing Process ◽  
Skin Lesions ◽  
Wound Healing Process ◽  
Primary Fibroblast ◽  
Fibroblast Migration ◽  
Healing Response ◽  
Wound Healing Response

AbstractDefective fibroblast migration causes delayed wound healing (WH) and chronic skin lesions. Autologous micrograft (AMG) therapies have recently emerged as a new effective treatment able to improve wound healing capacity. However, the molecular mechanisms connecting their beneficial outcomes with the wound healing process are still unrevealed. Here, we show that AMG modulates primary fibroblast migration and accelerates skin re-epithelialization without affecting cell proliferation. We demonstrate that AMG is enriched in a pool of WH-associated growth factors that may provide the initiation signal for a faster endogenous wound healing response. This, in turn leads to increased cell migration rate by elevating activity of extracellular signal-regulated kinase (ERK) pathway and subsequent activation of matrix metalloproteinase expression and their extracellular enzymatic activity. Moreover, AMG-treated wounds showed increased granulation tissue formation and organized collagen content. Overall, we shed light on AMG molecular mechanism supporting its potential to trigger a highly improved wound healing process.

Healing gone wrong: convergence of hemostatic pathways and liver fibrosis?

2020 ◽  
Vol 134 (16) ◽  
pp. 2189-2201
Author(s):  
Jessica P.E. Davis ◽  
Stephen H. Caldwell
Keyword(s):  
Wound Healing ◽  
Liver Disease ◽  
Hepatic Fibrosis ◽  
Cell Activation ◽  
Stellate Cell ◽  
Factor Xa ◽  
Clinical Trial Data ◽  
Chronic Liver ◽  
Healing Response ◽  
Wound Healing Response

Abstract Fibrosis results from a disordered wound healing response within the liver with activated hepatic stellate cells laying down dense, collagen-rich extracellular matrix that eventually restricts liver hepatic synthetic function and causes increased sinusoidal resistance. The end result of progressive fibrosis, cirrhosis, is associated with significant morbidity and mortality as well as tremendous economic burden. Fibrosis can be conceptualized as an aberrant wound healing response analogous to a chronic ankle sprain that is driven by chronic liver injury commonly over decades. Two unique aspects of hepatic fibrosis – the chronic nature of insult required and the liver’s unique ability to regenerate – give an opportunity for pharmacologic intervention to stop or slow the pace of fibrosis in patients early in the course of their liver disease. Two potential biologic mechanisms link together hemostasis and fibrosis: focal parenchymal extinction and direct stellate cell activation by thrombin and Factor Xa. Available translational research further supports the role of thrombosis in fibrosis. In this review, we will summarize what is known about the convergence of hemostatic changes and hepatic fibrosis in chronic liver disease and present current preclinical and clinical data exploring the relationship between the two. We will also present clinical trial data that underscores the potential use of anticoagulant therapy as an antifibrotic factor in liver disease.

Modulation Of Wound Healing Response In Chronic Irradiated Tissues

1993 ◽  
Vol 20 (3) ◽  
pp. 465-472 ◽  
Author(s):  
Thomas A. Mustoe ◽  
Beatriz H. Porras-Reyes
Keyword(s):  
Wound Healing ◽  
Healing Response ◽  
Wound Healing Response

Corneal Femtosecond Laser Keratotomy Results in Isolated Stromal Injury and Favorable Wound-Healing Response

2007 ◽  
Vol 48 (5) ◽  
pp. 2068 ◽  
Author(s):  
Christian Meltendorf ◽  
Guido J. Burbach ◽  
Jens Bu¨hren ◽  
Reinhold Bug ◽  
Christian Ohrloff ◽  
...  
Keyword(s):  
Wound Healing ◽  
Femtosecond Laser ◽  
Healing Response ◽  
Wound Healing Response

scholarly journals Effects of Laser at 810 Nm on Wound Healing in Albino Mice

2020 ◽  
pp. 23-33
Author(s):  
Mawada M. Funjan
Keyword(s):  
Wound Healing ◽  
Laser Therapy ◽  
Granulation Tissue ◽  
Near Infrared ◽  
Histological Evaluation ◽  
Control Groups ◽  
Respective Control ◽  
Healing Response ◽  
Wound Healing Response ◽  
Albino Mice

Many researches focused on laser therapy of wound healing in different animal models due to the lack of a standard protocol in the application of such phototherapy. Objective:  To study the effects of 810nm laser at a constant irradiance of 41.63 mw/cm2 and exposure (illumination) time of 5,15  minutes on wounds created on Albino mice (BALB/c).      Skin wound with elliptic shape and full thickness was created on the dorsal side of  ‘45 mature male albino mice. Irradiated animals were divided into two main groups based on irradiation time, the first was irradiated for 5 min and the second for 15 min, each was subdivided into three subgroups (n=5) according to number of treatment days (3, 5 and 10 days).   Both treated and respective control (n=15) subgroups were sacrificed on days 3, 5 and 10 posttreatment.  Laser therapy was applied using a 810 nm diode laser with a continuous wave, an output power of 400 mw, and irradiance of 41.63. The 5 min dose was 12 .5 J/cm2, whereas the 15 min dose was 37.4 J/cm2. The shape of the laser beam was fitted with a convex lens as ‘beam expander’ to irradiate a circular area of 3.4 cm diameter. Laser therapy was started after surgery and repeated for 3, 5 and 10 days, while its effects were examined by histological evaluation. Results:  At day 3 of treatment with near infrared 810nm laser at doses of 12.5J/cm² and 37.4J/cm², there was no evidence of wounds healing in irradiated groups which showed no differences with the respective control groups. At day 5 of treatment, the results showed an important increase in the scores of the parameters of wound healing (formation of granulation tissue and collagen deposition) in the irradiated groups. Near infrared 810nm laser had photobiostimulation effects on wound healing at irradiance of 41.63mW/cm² and doses of 12.5J/cm² for 5 minutes and 37.4J/cm² for 15 minutes exposure time. A complete picture of wound healing response appeared in all irradiated groups within 10 days of treatment, as expressed by complete ‘re-epithelialization’, moderate granulation tissue formation, and presence of collagen fibers, while incomplete wound healing response was observed in un-irradiated control groups within the same period. The study showed that 810 nm laser therapies had significant effects on wound healing, especially at a dose of 37.4J/cm².

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