Newly Synthesized Proteins in a Cell-Free System Supplemented with Human and Bovine Membrane-Bound Polysomes Isolated from Lens Fiber Cells

1988 ◽  
Vol 20 (6) ◽  
pp. 363-367 ◽  
Author(s):  
Lieko Ozaki
Keyword(s):  
Lens Fiber ◽  
Free System ◽  
Cell Free System ◽  
Fiber Cells ◽  
A Cell ◽  
Membrane Bound

The properties of free and membrane bound polysomes studied in a cell free system of Bombix mori silkglands

FEBS Letters ◽  
1971 ◽  
Vol 13 (6) ◽  
pp. 321-324 ◽  
Author(s):  
J. Daillie ◽  
L. Grasset ◽  
J.-C. Beck ◽  
J.-P. Prudhomme ◽  
J.-P. Ebel
Keyword(s):  
Free System ◽  
Cell Free System ◽  
A Cell ◽  
Membrane Bound

scholarly journals A Role for the Vesicle Tethering Protein, P115, in the Post-Mitotic Stacking of Reassembling Golgi Cisternae in a Cell-Free System

1999 ◽  
Vol 146 (1) ◽  
pp. 57-70 ◽  
Author(s):  
James Shorter ◽  
Graham Warren
Keyword(s):  
Temporal Analysis ◽  
Heterogeneous Population ◽  
Initial Alignment ◽  
Attachment Protein ◽  
Free System ◽  
Cell Free System ◽  
Vesicle Tethering ◽  
Protein Receptors ◽  
A Cell ◽  
Membrane Bound

During telophase, Golgi cisternae are regenerated and stacked from a heterogeneous population of tubulovesicular clusters. A cell-free system that reconstructs these events has revealed that cisternal regrowth requires interplay between soluble factors and soluble N-ethylmaleimide (NEM)–sensitive fusion protein (NSF) attachment protein receptors (SNAREs) via two intersecting pathways controlled by the ATPases, p97 and NSF. Golgi reassembly stacking protein 65 (GRASP65), an NEM-sensitive membrane-bound component, is required for the stacking process. NSF-mediated cisternal regrowth requires a vesicle tethering protein, p115, which we now show operates through its two Golgi receptors, GM130 and giantin. p97-mediated cisternal regrowth is p115-independent, but we now demonstrate a role for p115, in conjunction with its receptors, in stacking p97 generated cisternae. Temporal analysis suggests that p115 plays a transient role in stacking that may be upstream of GRASP65-mediated stacking. These results implicate p115 and its receptors in the initial alignment and docking of single cisternae that may be an important prerequisite for stack formation.

scholarly journals Effect of detergents on sterol synthesis in a cell-free system of yeast

1982 ◽  
Vol 23 (6) ◽  
pp. 803-810
Author(s):  
S Hata ◽  
T Nishino ◽  
N Ariga ◽  
H Katsuki
Keyword(s):  
Sterol Synthesis ◽  
Free System ◽  
Cell Free System ◽  
A Cell

scholarly journals Reconstitution of Endosomal Proteolysis in a Cell-free System

1989 ◽  
Vol 264 (10) ◽  
pp. 5392-5399
Author(s):  
L S Mayorga ◽  
R Diaz ◽  
P D Stahl
Keyword(s):  
Free System ◽  
Cell Free System ◽  
A Cell
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