Enzyme Activity Patterns and some Intermediates in Human Senile Cataract

1972 ◽  
Vol 3 (4) ◽  
pp. 224-232 ◽  
Author(s):  
D. Friedburg
Keyword(s):  
Enzyme Activity ◽  
Activity Patterns ◽  
Senile Cataract

CYCLISCHE SCHWANKUNGEN DER AKTIVITÄTEN VON HYDROXYSTEROID-DEHYDROGENASEN IM CORPUS LUTEUM DES RINDES

1972 ◽  
Vol 69 (2) ◽  
pp. 369-384
Author(s):  
H. Brandau ◽  
L. Brandau ◽  
G. Mutzke
Keyword(s):  
Enzyme Activity ◽  
Corpus Luteum ◽  
Enzyme Activities ◽  
Optical Method ◽  
Activity Patterns ◽  
Hydroxysteroid Dehydrogenase ◽  
Corpora Lutea ◽  
Bovine Corpus Luteum ◽  
Glucose 6 Phosphate Dehydrogenase

ABSTRACT In the bovine corpora lutea periodical activities of the Δ53β-, 3β-, 17β-and 20β-hydroxysteroid dehydrogenase (OHSDH) as well as activities of glyceraldehyde-3-phosphate- and glucose-6-phosphate dehydrogenase were measured quantitatively and the alterations throughout the different stages of the cycle were studied. After homogenization of the tissue and fractionate centrifugation the enzyme activities were determined by a standardized optical method. The activities of the Δ53β-, and 3β- and 17β-OHSDH increase slowly during the first 7 days of the cycle, the maximum is reached abruptly on the 12th to 13th day of the cycle. After a striking reduction the activities decline continually to the 19th to 21st day reaching the values detected at the beginning of the cycle. The 20β-OHSDH increases slowly to the maximum on the 15th day of the cycle. Activities of the 3α-OHSDH were obtained only inconsistently. The behaviour of the activities of G6PDH was nearly identical with that of the 3β-OHSDH, while the GAPDH shows only little fluctuations of its activities. The obtained enzyme activity patterns of the maturating and high functional corpus luteum correspond to the well-known data of the biosynthetic function of the bovine corpus luteum. The changes of the amounts of progesterone and 20β-progesterol agree with the course of the activities of the 3β- resp. 20β-OHSDH.

Enzyme activity patterns of energy metabolism in skiers of different performance levels (M. quadriceps femoris)

1982 ◽  
Vol 48 (3) ◽  
pp. 315-322 ◽  
Author(s):  
Eva V. Macková ◽  
Arnošt Bass ◽  
štĚpánka šprynarová ◽  
Jan Teisinger ◽  
Karel Vondra ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Energy Metabolism ◽  
Activity Patterns ◽  
Quadriceps Femoris ◽  
Performance Levels

Enzyme activity patterns of energy supplying metabolism in the quadriceps femoris muscle(Vastus lateralis)

1976 ◽  
Vol 361 (2) ◽  
pp. 169-173 ◽  
Author(s):  
Arnošt Bass ◽  
Karel Vondra ◽  
Ratmír Rath ◽  
Václav Vítek ◽  
Jan Teisinger ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Vastus Lateralis ◽  
Activity Patterns ◽  
Quadriceps Femoris ◽  
Quadriceps Femoris Muscle ◽  
Femoris Muscle

scholarly journals Reduced glutathione level and gsh-dependent enzyme activities in corticonuclear blocks of lenses in patients with senile cataract

2012 ◽  
Vol 140 (9-10) ◽  
pp. 563-570 ◽  
Author(s):  
Bojana Kisic ◽  
Dijana Miric ◽  
Lepsa Zoric ◽  
Aleksandra Ilic ◽  
Ilija Dragojevic
Keyword(s):  
Enzyme Activity ◽  
Cumene Hydroperoxide ◽  
Thiol Group ◽  
Normal Function ◽  
Senile Cataract ◽  
Lens Epithelium ◽  
Cell Permeability ◽  
Ocular Tissue ◽  
Glutathione S Transferase ◽  
Mature Cataract

Introduction. Reduced compound glutathione (GSH) in the lens has the function to protect the thiol group of lens proteins, and as a substrate of glutathione peroxidase (GPx) and glutathione S-transferase (GST). Protein containing thiol groups is significant for the normal function of lens epithelium, i.e. enzymes Na-K-ATP-ase, thus influencing cell permeability. The relationship GSH/GSSG (oxidized glutathione) is normally high in the lens and other ocular tissue owing to the glutathioneredox cycle, which is localized in the lens epithelium and cortex surface. Objective. The aim of the study was to investigate non-enzymic factors of the antioxidant protection of non-protein and protein tiol, as well as to determine glutathione-dependent enzyme activity in the corticonuclear blocks of lenses in patients with senile cataract. Methods. Biochemical studies of lens were carried on 101 patients with senile cataract. According to cataract maturity degree, the patients were classified into two groups: senile incipient cataract (N=41) and mature senile cataract (N=60). GSH concentration was determined by Ellman?s reagent. GPx activity was assayed with cumene hydroperoxide, and that of glutathione S-transferase by follow-up of glutathione conjugation and 1-chloro-2.4-dinitrobenzene rates. Results. A significantly higher GSH concentration was found in the corticonuclear blocks of lenses with initial as related to mature cataract (p<0.001). The activity of enzyme GPx and GST was considerably higher in the corticonuclear blocks of lenses with initial cataract (p<0.001). With cataract progression, the quantity of available GSH, necessary for GPx and GST functioning, declined, so that the activity of these enzymes was also significantly decreased in mature cataract. Conclusion. The determined lower GSH concentration and antioxidant enzyme activity in corticonuclear blocks of lenses, particularly in cataract with a nuclear component, indicate the weakened antioxidant response of lens tissue during the development of senile cataract.

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