Mitosis of Normal Human Lens Epithelium in vitro

1983 ◽  
Vol 15 (6) ◽  
pp. 314-317
Author(s):  
K. Cansu
Keyword(s):  
Lens Epithelium ◽  
Human Lens ◽  
Normal Human ◽  
Human Lens Epithelium

Transfection of CTGF siRNA inhibits transdifferentiation in human lens epithelium cell line B3 in vitro

2020 ◽  
Vol 1 (1) ◽  
pp. 17-23
Author(s):  
Hua Zhuang ◽  
◽  
Jing Wu ◽  
Wei Xu ◽  
Jian-Zhang Hu ◽  
...  
Keyword(s):  
Smooth Muscle Actin ◽  
Negative Control ◽  
Tissue Growth ◽  
Lens Epithelium ◽  
Cell Counting ◽  
Human Lens ◽  
Posterior Capsular Opacification ◽  
Epithelium Cell ◽  
Human Lens Epithelium

AIM: To investigate the expression of connective tissue growth factor (CTGF) and α-smooth muscle actin (α-SMA) in human lens epithelium cell (HLEC) line B3 after transfection by liposome-coated small interfering RNA (siRNA) targeting CTGF. METHODS: HLECs were transfected with siRNA targeting CTGF, labeled with 5’-fluorescein isothiocyanate (5’-FITC) and coated with lipofectamine. The transfection ratio was evaluated via fluorescence intensity. Cell counting kit-8 (CCK-8) assay was performed to assess cytoviability of both non-transfected and transfected HLECs. Quantitative reverse transcription-polymerase chain reaction (RT-PCR), cell immunochemistry and Western blot analysis were conducted to detect the expression changes of CTGF and α-SMA after transfection. RESULTS: A highly effective transfection ratio was observed in siRNA co-transfected with lipofectamine. The transfection ratio reached 95% at 24h. The proliferation of HLECs was inhibited by siRNA after 72h transfection. The expression of CTGF and α-SMA significantly decreased in HLECs after transfected by CTGF siRNA for 24h. This effect was not found in negative control siRNA. CONCLUSION: siRNA targeting CTGF decrease CTGF and α-SMA expression in HLECs, which is a potential therapeutic strategy for posterior capsular opacification.

scholarly journals Somatic Variants in the Human Lens Epithelium: A Preliminary Assessment

2016 ◽  
Vol 57 (10) ◽  
pp. 4063 ◽  
Author(s):  
Rosana Mesa ◽  
Manoj Tyagi ◽  
George Harocopos ◽  
David Vollman ◽  
Steven Bassnett
Keyword(s):  
Lens Epithelium ◽  
Human Lens ◽  
Preliminary Assessment ◽  
Human Lens Epithelium

An Immunoperoxidase Study of Human Lens Epithelium

1990 ◽  
Vol 2 (1) ◽  
pp. 71-73 ◽  
Author(s):  
Margaret J. Jeffrey ◽  
W.T. Green ◽  
D.L. Boase ◽  
M.N. Jeffrey
Keyword(s):  
Lens Epithelium ◽  
Human Lens ◽  
Human Lens Epithelium

scholarly journals Ethylene glycol coated nanoceria protects against oxidative stress in human lens epithelium

RSC Advances ◽  
2019 ◽  
Vol 9 (29) ◽  
pp. 16596-16605 ◽  
Author(s):  
Belal I. Hanafy ◽  
Gareth W. V. Cave ◽  
Yvonne Barnett ◽  
Barbara Pierscionek
Keyword(s):  
Oxidative Stress ◽  
Ethylene Glycol ◽  
Lens Epithelium ◽  
Human Lens ◽  
Human Lens Epithelium

Nanoceria (<5 nm), stabilised with ethylene glycol, protects human lens epithelium from oxidative stress and exhibits multicoloured photoluminescence.

Upregulation of miR-630 Induced by Oxidative Damage Resists Cell Migration Through Targeting ALCAM in Human Lens Epithelium Cells

2019 ◽  
Vol 45 (2) ◽  
pp. 153-161 ◽  
Author(s):  
Lin Mei ◽  
Hong Yan ◽  
Song Wang ◽  
Chenjun Guo ◽  
Xiaoliang Zheng ◽  
...  
Keyword(s):  
Cell Migration ◽  
Oxidative Damage ◽  
Lens Epithelium ◽  
Human Lens ◽  
Human Lens Epithelium ◽  
Epithelium Cells
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