Effect of pH on Acid Production from Sorbitol in Washed Cell Suspensions of Oral Bacteria

1990 ◽  
Vol 24 (2) ◽  
pp. 107-112 ◽  
Author(s):  
S. Kalfas ◽  
Y. Maki ◽  
D. Birkhed ◽  
S. Edwardsson
Keyword(s):  
Acid Production ◽  
Oral Bacteria ◽  
Cell Suspensions ◽  
Effect Of Ph ◽  
Washed Cell

scholarly journals Starvation Survival and Biofilm Formation under Subminimum Inhibitory Concentration of QAMs

2021 ◽  
Vol 2021 ◽  
pp. 1-10
Author(s):  
Sanjay Kumar Tiwari ◽  
Suping Wang ◽  
Yannan Huang ◽  
Xuedong Zhou ◽  
Hockin H. K. Xu ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Biofilm Formation ◽  
Acid Production ◽  
Inhibitory Concentration ◽  
Lactic Acid Production ◽  
Oral Bacteria ◽  
Laser Scanning Microscopy ◽  
Prolonged Starvation ◽  
Survival And Development ◽  
Development Of Tolerance

Quaternary ammonium methacrylates (QAMs) are useful antimicrobial compounds against oral bacteria. Here, we investigated the effects of two QAMs, dimethylaminododecyl methacrylate (DMADDM) and dimethylaminohexadecyl methacrylate (DMAHDM), on biofilm formation, survival and development of tolerance by biofilm, and survival and development of tolerance against QAMs after prolonged starvation. Enterococcus faecalis (E. faecalis), Streptococcus gordonii (S. gordonii), Lactobacillus acidophilus (L. acidophilus), and Actinomyces naeslundii (A. naeslundii) were used. Minimum inhibitory concentration (MIC) of QAMs against multispecies biofilm was determined. Biofilm formed under sub-MIC was observed by crystal violet staining and confocal laser scanning microscopy (CLSM). Metabolic activity was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and lactic acid production measurement. Development of tolerance was determined by MIC values before and after exposure to QAMs or after prolonged starvation. It was found that E. faecalis and S. gordonii could survive and form biofilm under sub-MIC of QAMs. Lactic acid production from biofilms formed under sub-MIC was significantly higher than control specimens ( p < 0.05 ). The exposure to sub-MIC of QAMs promoted biofilm formation, and prolonged starvation or prolonged contact with sub-MIC helped bacteria develop tolerance against killing by QAMs.

The sesquiterpene botrydial from Botrytis cinerea induces phosphatidic acid production in tomato cell suspensions

Planta ◽  
2018 ◽  
Vol 247 (4) ◽  
pp. 1001-1009 ◽  
Author(s):  
Juan Martin D’Ambrosio ◽  
Gabriela Gonorazky ◽  
Daniela J. Sueldo ◽  
Javier Moraga ◽  
Andrés Arruebarrena Di Palma ◽  
...  
Keyword(s):  
Phosphatidic Acid ◽  
Botrytis Cinerea ◽  
Acid Production ◽  
Cell Suspensions

scholarly journals Polyphosphate-Accumulating Bacteria: Potential Contributors to Mineral Dissolution in the Oral Cavity

2018 ◽  
Vol 84 (7) ◽  
Author(s):  
Ashley A. Breiland ◽  
Beverly E. Flood ◽  
Julia Nikrad ◽  
John Bakarich ◽  
Matthew Husman ◽  
...  
Keyword(s):  
Oral Cavity ◽  
Acid Production ◽  
Phosphate Uptake ◽  
Model Organism ◽  
Oral Bacteria ◽  
Mineral Dissolution ◽  
Public Health Issue ◽  
Saturation State ◽  
Content Type ◽  
Carious Lesions

ABSTRACT Bacteria that accumulate polyphosphates have previously been shown to dynamically influence the solubility of phosphatic minerals in marine settings and wastewater. Here, we show that dental plaque, saliva, and carious lesions all contain abundant polyphosphate-accumulating bacteria. Saturation state modeling results, informed by phosphate uptake experiments using the model organism Lactobacillus rhamnosus , which is known to inhabit advanced carious lesions, suggest that polyphosphate accumulation can lead to undersaturated conditions with respect to hydroxyapatite under some oral cavity conditions. The cell densities of polyphosphate-accumulating bacteria we observed in some regions of oral biofilms are comparable to those that produce undersaturated conditions (i.e., those that thermodynamically favor mineral dissolution) in our phosphate uptake experiments with L. rhamnosus . These results suggest that the localized generation of undersaturated conditions by polyphosphate-accumulating bacteria constitutes a new potential mechanism of tooth dissolution that may augment the effects of metabolic acid production. IMPORTANCE Dental caries is a serious public health issue that can have negative impacts on overall quality of life and oral health. The role of oral bacteria in the dissolution of dental enamel and dentin that can result in carious lesions has long been solely ascribed to metabolic acid production. Here, we show that certain oral bacteria may act as a dynamic shunt for phosphate in dental biofilms via the accumulation of a polymer known as polyphosphate—potentially mediating phosphate-dependent conditions such as caries (dental decay).

Propionic acid production from food waste in batch reactors: Effect of pH, types of inoculum, and thermal pre-treatment

2021 ◽  
Vol 319 ◽  
pp. 124166
Author(s):  
Rowayda Ali ◽  
Florencia Saravia ◽  
Andrea Hille-Reichel ◽  
Johannes Gescher ◽  
Harald Horn
Keyword(s):  
Propionic Acid ◽  
Food Waste ◽  
Acid Production ◽  
Batch Reactors ◽  
Effect Of Ph ◽  
Propionic Acid Production ◽  
Pre Treatment

scholarly journals The synthesis of indole by washed cell suspensions of Escherichia coli

1956 ◽  
Vol 64 (1) ◽  
pp. 132-137 ◽  
Author(s):  
F. Gibson ◽  
Marjorie J. Jones ◽  
H. Teltscher
Keyword(s):  
Escherichia Coli ◽  
Cell Suspensions ◽  
Washed Cell

scholarly journals Effect of Linoleic Acid Concentration on Conjugated Linoleic Acid Production by Butyrivibrio fibrisolvensA38

2000 ◽  
Vol 66 (12) ◽  
pp. 5226-5230 ◽  
Author(s):  
Young Jun Kim ◽  
Rui Hai Liu ◽  
Daniel R. Bond ◽  
James B. Russell
Keyword(s):  
Linoleic Acid ◽  
Conjugated Linoleic Acid ◽  
Cell Density ◽  
Cell Suspensions ◽  
Bacterial Inactivation ◽  
Butyrivibrio Fibrisolvens ◽  
Washed Cell ◽  
Initial Cell ◽  
Normal Enzyme ◽  
Linoleic Acid Concentration

ABSTRACT Butyrivibrio fibrisolvens A38 inocula were inhibited by as little as 15 μM linoleic acid (LA), but growing cultures tolerated 10-fold more LA before growth was inhibited. Growing cultures did not produce significant amounts of cis-9, trans-11 conjugated linoleic acid (CLA) until the LA concentration was high enough to inhibit biohydrogenation, growth was inhibited, and lysis was enhanced. Washed-cell suspensions that were incubated anaerobically with 350 μM LA converted most of the LA to hydrogenated products, and little CLA was detected. When the washed-cell suspensions were incubated aerobically, biohydrogenation was inhibited, CLA production was at least twofold greater, and CLA persisted. The LA isomerase reaction was very rapid, but the LA isomerase did not recycle like a normal enzyme to catalyze more substrate. Cells that were preincubated with CLA lost their ability to produce more CLA from LA, and the CLA accumulation was directly proportional (r 2= 0.98) to the initial cell density. Growing cells were as sensitive to CLA as LA, the LA isomerase and reductases of biohydrogenation were linked, and free CLA was not released. Because growing cultures ofB. fibrisolvens A38 did not produce significant amounts of CLA until the LA concentration was high, biohydrogenation was arrested, and the cell density had declined, the flow of CLA from the rumen may be due to LA-dependent bacterial inactivation, death, or lysis.

scholarly journals The Effect of pH and Temperature on Arachidonic Acid Production by Glycerol-Grown Mortierella alpina NRRL-A-10995

Fermentation ◽  
2018 ◽  
Vol 4 (1) ◽  
pp. 17 ◽  
Author(s):  
Aleksei Mironov ◽  
Vitaly Nemashkalov ◽  
Nadezda Stepanova ◽  
Svetlana Kamzolova ◽  
Waldemar Rymowicz ◽  
...  
Keyword(s):  
Arachidonic Acid ◽  
Acid Production ◽  
Mortierella Alpina ◽  
Effect Of Ph ◽  
Arachidonic Acid Production
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