Cross-Species Chromosome Painting Corroborates Microchromosome Fusion during Karyotype Evolution of Birds

2009 ◽  
Vol 126 (3) ◽  
pp. 281-304 ◽  
Author(s):  
T. Hansmann ◽  
I. Nanda ◽  
V. Volobouev ◽  
F. Yang ◽  
M. Schartl ◽  
...  
Keyword(s):  
Chromosome Painting ◽  
Karyotype Evolution ◽  
Evolution Of Birds ◽  
Cross Species Chromosome Painting

Chromosome evolution in kangaroos (Marsupialia: Macropodidae): Cross species chromosome painting between the tammar wallaby and rock wallaby spp. with the 2n = 22 ancestral macropodid karyotype

Genome ◽  
1999 ◽  
Vol 42 (3) ◽  
pp. 525-530 ◽  
Author(s):  
RJ Waugh O'Neill ◽  
MDB Eldridge ◽  
R Toder ◽  
MA Ferguson-Smith ◽  
P C O'Brien ◽  
...  
Keyword(s):  
Chromosome Painting ◽  
Chromosome Evolution ◽  
Karyotype Evolution ◽  
Tammar Wallaby ◽  
Fusion Process ◽  
Ancestral Karyotype ◽  
Karyotypic Diversity ◽  
Species Groups ◽  
Cross Species Chromosome Painting ◽  
Karyotype Stability

Marsupial mammals show extraordinary karyotype stability, with 2n = 14 considered ancestral. However, macropodid marsupials (kangaroos and wallabies) exhibit a considerable variety of karyotypes, with a hypothesised ancestral karyotype of 2n = 22. Speciation and karyotypic diversity in rock wallabies (Petrogale) is exceptional. We used cross species chromosome painting to examine the chromosome evolution between the tammar wallaby (2n = 16) and three 2n = 22 rock wallaby species groups with the putative ancestral karyotype. Hybridization of chromosome paints prepared from flow sorted chromosomes of the tammar wallaby to Petrogale spp., showed that this ancestral karyotype is largely conserved among 2n = 22 rock wallaby species, and confirmed the identity of ancestral chromosomes which fused to produce the bi-armed chromosomes of the 2n = 16 tammar wallaby. These results illustrate the fission-fusion process of karyotype evolution characteristic of the kangaroo group.

Karyotype evolution in Rhinolophus bats (Rhinolophidae, Chiroptera) illuminated by cross-species chromosome painting and G-banding comparison

2007 ◽  
Vol 15 (7) ◽  
pp. 835-848 ◽  
Author(s):  
Xiuguang Mao ◽  
Wenhui Nie ◽  
Jinhuan Wang ◽  
Weiting Su ◽  
Lei Ao ◽  
...  
Keyword(s):  
Chromosome Painting ◽  
Karyotype Evolution ◽  
Cross Species Chromosome Painting

Cross-species chromosome painting in Cetartiodactyla: Reconstructing the karyotype evolution in key phylogenetic lineages

2009 ◽  
Vol 17 (3) ◽  
pp. 419-436 ◽  
Author(s):  
Anastasia I. Kulemzina ◽  
Vladimir A. Trifonov ◽  
Polina L. Perelman ◽  
Nadezhda V. Rubtsova ◽  
Vitaly Volobuev ◽  
...  
Keyword(s):  
Chromosome Painting ◽  
Karyotype Evolution ◽  
Phylogenetic Lineages ◽  
Cross Species Chromosome Painting

Phylogenomic study of the subfamily Caprinae by cross-species chromosome painting with Chinese muntjac paints

2005 ◽  
Vol 13 (4) ◽  
pp. 389-399 ◽  
Author(s):  
Ling Huang ◽  
Wenhui Nie ◽  
Jinhuan Wang ◽  
Weiting Su ◽  
Fengtang Yang
Keyword(s):  
Chromosome Painting ◽  
Cross Species Chromosome Painting ◽  
Phylogenomic Study

scholarly journals Marsupial chromosome DNA content and genome size assessed from flow karyotypes: invariable low autosomal GC content

2018 ◽  
Vol 5 (8) ◽  
pp. 171539 ◽  
Author(s):  
Fumio Kasai ◽  
Patricia C. M. O'Brien ◽  
Jorge C. Pereira ◽  
Malcolm A. Ferguson-Smith
Keyword(s):  
Genome Size ◽  
Dna Content ◽  
Chromosome Painting ◽  
Gc Content ◽  
Tammar Wallaby ◽  
Individual Chromosome ◽  
Tasmanian Devil ◽  
Marsupial Species ◽  
Cross Species Chromosome Painting ◽  
High Level

Extensive chromosome homologies revealed by cross-species chromosome painting between marsupials have suggested a high level of genome conservation during evolution. Surprisingly, it has been reported that marsupial genome sizes vary by more than 1.2 Gb between species. We have shown previously that individual chromosome sizes and GC content can be measured in flow karyotypes, and have applied this method to compare four marsupial species. Chromosome sizes and GC content were calculated for the grey short-tailed opossum (2 n = 18), tammar wallaby (2 n = 16), Tasmanian devil (2 n = 14) and fat-tailed dunnart (2 n = 14), resulting in genome sizes of 3.41, 3.31, 3.17 and 3.25 Gb, respectively. The findings under the same conditions allow a comparison between the four species, indicating that the genomes of these four species are 1–8% larger than human. We show that marsupial genomes are characterized by a low GC content invariable between autosomes and distinct from the higher GC content of the marsupial × chromosome.

scholarly journals Isolating Chromosomes of the Komodo Dragon: New Tools for Comparative Mapping and Sequence Assembly

2019 ◽  
Vol 157 (1-2) ◽  
pp. 123-131 ◽  
Author(s):  
Alessio Iannucci ◽  
Marie Altmanová ◽  
Claudio Ciofi ◽  
Malcolm Ferguson-Smith ◽  
Jorge C. Pereira ◽  
...  
Keyword(s):  
Karyotype Evolution ◽  
Sequencing Technology ◽  
Next Generation Sequencing Technology ◽  
Flow Karyotype ◽  
Flow Sorting ◽  
Dna Pools ◽  
Phylogenomic Analyses ◽  
Cross Species Chromosome Painting ◽  
Chromosomal Map ◽  
Generation Sequencing

We developed new tools to build a high-quality chromosomal map of the Komodo dragon (Varanus komodoensis) available for cross-species phylogenomic analyses. First, we isolated chromosomes by flow sorting and determined the chromosome content of each flow karyotype peak by FISH. We then isolated additional Komodo dragon chromosomes by microdissection and amplified chromosome-specific DNA pools. The chromosome-specific DNA pools can be sequenced, assembled, and mapped by next-generation sequencing technology. The chromosome-specific paint probes can be used to investigate karyotype evolution through cross-species chromosome painting. Overall, the set of chromosome-specific DNA pools of V. komodoensis provides new tools for detailed phylogenomic analyses of Varanidae and squamates in general.

scholarly journals Karyotype Evolution in Birds: From Conventional Staining to Chromosome Painting

Genes ◽  
2018 ◽  
Vol 9 (4) ◽  
pp. 181 ◽  
Author(s):  
Rafael Kretschmer ◽  
Malcolm Ferguson-Smith ◽  
Edivaldo de Oliveira
Keyword(s):  
Chromosome Painting ◽  
Karyotype Evolution

scholarly journals Chromosome painting among Proboscidea, Hyracoidea and Sirenia: support for Paenungulata (Afrotheria, Mammalia) but not Tethytheria

2007 ◽  
Vol 274 (1615) ◽  
pp. 1333-1340 ◽  
Author(s):  
A.T Pardini ◽  
P.C.M O'Brien ◽  
B Fu ◽  
R.K Bonde ◽  
F.F.B Elder ◽  
...  
Keyword(s):  
Chromosome Painting ◽  
Phylogenetic Signal ◽  
Chromosomal Evolution ◽  
Rapid Radiation ◽  
Trichechus Manatus Latirostris ◽  
Chromosomal Change ◽  
Outgroup Taxon ◽  
Procavia Capensis ◽  
Reduced Rate ◽  
Cross Species Chromosome Painting

Despite marked improvements in the interpretation of systematic relationships within Eutheria, particular nodes, including Paenungulata (Hyracoidea, Sirenia and Proboscidea), remain ambiguous. The combination of a rapid radiation, a deep divergence and an extensive morphological diversification has resulted in a limited phylogenetic signal confounding resolution within this clade both at the morphological and nucleotide levels. Cross-species chromosome painting was used to delineate regions of homology between Loxodonta africana (2 n =56), Procavia capensis (2 n =54), Trichechus manatus latirostris (2 n =48) and an outgroup taxon, the aardvark ( Orycteropus afer , 2 n =20). Changes specific to each lineage were identified and although the presence of a minimum of 11 synapomorphies confirmed the monophyly of Paenungulata, no change characterizing intrapaenungulate relationships was evident. The reconstruction of an ancestral paenungulate karyotype and the estimation of rates of chromosomal evolution indicate a reduced rate of genomic repatterning following the paenungulate radiation. In comparison to data available for other mammalian taxa, the paenungulate rate of chromosomal evolution is slow to moderate. As a consequence, the absence of a chromosomal character uniting two paenungulates (at the level of resolution characterized in this study) may be due to a reduced rate of chromosomal change relative to the length of time separating successive divergence events.

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