scholarly journals Phospholipase A2 Activity of Peripheral-Blood Mononuclear Leukocytes in Psoriasis

Dermatology ◽  
1995 ◽  
Vol 190 (4) ◽  
pp. 318-319
Author(s):  
R.O. Leder ◽  
S. Vossough ◽  
H. Weltman ◽  
D.I. Wilkinson
Keyword(s):  
Peripheral Blood ◽  
Phospholipase A ◽  
Mononuclear Leukocytes ◽  
Peripheral Blood Mononuclear

Arachidonic acid and LTB4 enhance aggregation of psoriatic peripheral blood mononuclear leukocytes in vitro

1987 ◽  
Vol 279 (5) ◽  
pp. 347-350 ◽  
Author(s):  
P. D. Pigatto ◽  
M. M. Polengni ◽  
G. F. Altomare ◽  
G. L. Tadini ◽  
S. Villa
Keyword(s):  
Arachidonic Acid ◽  
Peripheral Blood ◽  
Mononuclear Leukocytes ◽  
Peripheral Blood Mononuclear

scholarly journals Expression of Matrix Metalloproteinase-2 and -9 and Their Inhibitors in Peripheral Blood Cells of Patients with Chronic Hepatitis C

2000 ◽  
Vol 46 (2) ◽  
pp. 183-192 ◽  
Author(s):  
Ralf Lichtinghagen ◽  
Omar Huegel ◽  
Thomas Seifert ◽  
Christian I Haberkorn ◽  
Dirk Michels ◽  
...  
Keyword(s):  
Hepatitis C ◽  
Peripheral Blood ◽  
Serum Markers ◽  
Peripheral Blood Cell ◽  
Chronic Liver ◽  
Matrix Metalloproteinase 2 ◽  
Mononuclear Leukocytes ◽  
Healthy Controls ◽  
Peripheral Blood Mononuclear ◽  
Progression Marker

Abstract Background: To clarify whether circulating matrix metalloproteinases (MMPs) and their tissue inhibitors (TIMPs) can be used as serum markers of fibroproliferation in chronic liver diseases, we studied the expression of MMP-2 and MMP-9 in relation to TIMP-1 and TIMP-2 in peripheral blood mononuclear leukocytes (MNLs) and polymorphonuclear leukocytes (PMLs), and compared this expression to circulating concentrations and hepatic histology in patients with chronic active hepatitis C (CAH). Methods: Quantitative reverse transcription-PCR/ELISA assays were performed for MMP and TIMP RNA, and corresponding circulating protein concentrations were studied by ELISA in 20 healthy controls, 40 patients with CAH, and 20 patients with hepatitis C-induced cirrhosis (Ci). Results: MMP-2 mRNA was found almost exclusively in the liver, MMP-9 mRNA in leukocytes. TIMP RNA-equivalents were decreased in MNLs of CAH patients, but neither MMP-9 nor TIMP RNA expression showed any correlation to the extent of inflammation and fibrosis. MMP-2 and TIMP-1 protein concentrations were increased in Ci patients and showed a wide overlap in CAH patients and healthy controls. MMP-9 values were lower in CAH and Ci patients than in healthy controls. TIMP-2 values showed a wide overlap in all three groups. The MMP-2/TIMP-1 and MMP-9/TIMP-1 ratios were lower in Ci patients than in healthy controls; the MMP-2/TIMP-2 and MMP-9/TIMP-2 ratios were not different. Circulating TIMP-1 and the MMP-2/TIMP-1 ratio correlated to the inflammatory activity in liver biopsies, but only the circulating MMP-2/TIMP-1 ratio also correlated with the degree of fibrosis. Conclusions: Peripheral blood cell expression of MMP-2, MMP-9, and TIMP revealed no correlation with the circulating concentrations of these proteins. Only the circulating MMP-2/TIMP-1 ratio correlated to the histological degree of fibrosis in hepatitis C and should be further evaluated as a progression marker in patients with chronic liver disease.

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