Insulin-Like Growth Factor 1 and Parathyroid Hormone Effects on the Growth of Fetal Rat Metatarsal Bones Cultured in Serum-Free Medium

Neonatology ◽  
1995 ◽  
Vol 68 (5) ◽  
pp. 368-376 ◽  
Author(s):  
V. Coxam ◽  
M.A. Miller ◽  
B.M. Bowman ◽  
D. Qi ◽  
S.C. Miller
Keyword(s):  
Parathyroid Hormone ◽  
Growth Factor ◽  
Insulin Like Growth Factor ◽  
Serum Free Medium ◽  
Free Medium ◽  
Serum Free ◽  
Metatarsal Bones ◽  
Fetal Rat ◽  
Hormone Effects

Platelet-derived growth factor and multiplication-stimulating activity II, but not multiplication-stimulating activity III-2, stimulate [3H]thymidine and [35S]sulphate incorporation by fetal rat costal cartilage in vitro

1984 ◽  
Vol 103 (2) ◽  
pp. 195-203 ◽  
Author(s):  
D. J. Hill ◽  
R. D. G. Milner
Keyword(s):  
Growth Factor ◽  
Costal Cartilage ◽  
Platelet Derived Growth Factor ◽  
Thymidine Uptake ◽  
Serum Free Medium ◽  
Free Medium ◽  
Serum Free ◽  
Fetal Rat ◽  
Rat Fibroblasts

ABSTRACT The actions of partially purified porcine platelet-derived growth factor (PDGF) and highly purified multiplication-stimulating activity (MSA) II and MSA III-2, which are somatomedins, were investigated on the incorporation of [3H]thymidine and [35S]sulphate by fetal rat costal cartilage in vitro. This was compared with their effects in the presence of 1% fetal calf serum (FCS) on the uptake of thymidine by growth-arrested fetal rat fibroblasts. Platelet-derived growth factor at concentrations of 0·21–21 μg/l enhanced the incorporation of both isotopes by fetal cartilage in the presence of 1% FCS, but had an inconsistent action on thymidine uptake and no significant action on sulphate uptake in serum-free medium. Platelet-derived growth factor promoted thymidine uptake by growth-arrested, isolated fetal rat fibroblasts. Multiplication-stimulating activity II (10–100 μg/l) stimulated the uptake of thymidine and sulphate by fetal cartilage in medium containing 1% FCS but had no consistent action in serum-free medium, although MSA II and PDGF had a synergistic effect on thymidine uptake in the absence of serum. Multiplication-stimulating activity III-2 had no consistent action on thymidine or sulphate incorporation by fetal cartilage in either serum-free or serum-supplemented medium. However, the same preparation of MSA III-2 stimulated the uptake of [3H]thymidine into fetal rat fibroblasts with a half-maximal response at a concentration of 5–10 μg/l. The results identify PDGF as a possible mitogenic agent for fetal rat connective tissues in vitro and show a differential sensitivity of fetal cartilage to MSA peptides. J. Endocr. (1984) 103, 195–203

scholarly journals Effect of a null mutation of the insulin-like growth factor I receptor gene on growth and transformation of mouse embryo fibroblasts.

1994 ◽  
Vol 14 (6) ◽  
pp. 3604-3612 ◽  
Author(s):  
C Sell ◽  
G Dumenil ◽  
C Deveaud ◽  
M Miura ◽  
D Coppola ◽  
...  
Keyword(s):  
Growth Factors ◽  
Growth Factor ◽  
Soft Agar ◽  
T Antigen ◽  
Insulin Like Growth Factor ◽  
Serum Free Medium ◽  
Free Medium ◽  
Serum Free ◽  
Factor I ◽  
Growth Factor I

Fibroblast cell lines, designated R- and W cells, were generated, respectively, from mouse embryos homozygous for a targeted disruption of the Igf1r gene, encoding the type 1 insulin-like growth factor receptor, and from their wild-type littermates. W cells grow normally in serum-free medium supplemented with various combinations of purified growth factors, while pre- and postcrisis R- cells cannot grow, as they are arrested before entering the S phase. R- cells are able to grow in 10% serum, albeit more slowly than W cells, and with all phases of the cell cycle being elongated. An activated Ha-ras expressed from a stably transfected plasmid is unable to overcome the inability of R- cells to grow in serum-free medium supplemented with purified clones. Nevertheless, even in the presence of serum, R- cells stably transfected with Ha-ras, alone or in combination with simian virus 40 large T antigen, fail to form colonies in soft agar. Reintroduction into R- cells (or their derivatives) of a plasmid expressing the human insulin-like growth factor I receptor RNA and protein restores their ability to grow with purified growth factors or in soft agar. The signaling pathways participating in cell growth and transformation are discussed on the basis of these results.

Effect of a null mutation of the insulin-like growth factor I receptor gene on growth and transformation of mouse embryo fibroblasts

1994 ◽  
Vol 14 (6) ◽  
pp. 3604-3612
Author(s):  
C Sell ◽  
G Dumenil ◽  
C Deveaud ◽  
M Miura ◽  
D Coppola ◽  
...  
Keyword(s):  
Growth Factors ◽  
Growth Factor ◽  
Soft Agar ◽  
T Antigen ◽  
Insulin Like Growth Factor ◽  
Serum Free Medium ◽  
Free Medium ◽  
Serum Free ◽  
Factor I ◽  
Growth Factor I

Fibroblast cell lines, designated R- and W cells, were generated, respectively, from mouse embryos homozygous for a targeted disruption of the Igf1r gene, encoding the type 1 insulin-like growth factor receptor, and from their wild-type littermates. W cells grow normally in serum-free medium supplemented with various combinations of purified growth factors, while pre- and postcrisis R- cells cannot grow, as they are arrested before entering the S phase. R- cells are able to grow in 10% serum, albeit more slowly than W cells, and with all phases of the cell cycle being elongated. An activated Ha-ras expressed from a stably transfected plasmid is unable to overcome the inability of R- cells to grow in serum-free medium supplemented with purified clones. Nevertheless, even in the presence of serum, R- cells stably transfected with Ha-ras, alone or in combination with simian virus 40 large T antigen, fail to form colonies in soft agar. Reintroduction into R- cells (or their derivatives) of a plasmid expressing the human insulin-like growth factor I receptor RNA and protein restores their ability to grow with purified growth factors or in soft agar. The signaling pathways participating in cell growth and transformation are discussed on the basis of these results.

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