Hypoxia-Ischemia in the Neonatal Rat Brain: Histopathology after Post-Treatment with NMDA and Non NMDA Receptor Antagonists

Neonatology ◽  
1994 ◽  
Vol 66 (4) ◽  
pp. 205-213 ◽  
Author(s):  
Henrik Hagberg ◽  
Eric Gilland ◽  
Nils-Henrik Diemer ◽  
Peter Andiné
Keyword(s):  
Nmda Receptor ◽  
Rat Brain ◽  
Post Treatment ◽  
Neonatal Rat ◽  
Nmda Receptor Antagonists ◽  
Receptor Antagonists ◽  
Hypoxia Ischemia ◽  
Neonatal Rat Brain

Use of Both Fluoro-Jade B and Hematoxylin and Eosin to Detect Cell Death in the Juvenile Rat Brain Exposed to NMDA-Receptor Antagonists or GABA-Receptor Agonists in Safety Assessment

2021 ◽  
pp. 019262332110077
Author(s):  
Catherine A. Picut ◽  
Odete R. Mendes ◽  
David S. Weil ◽  
Sarah Davis ◽  
Cynthia Swanson
Keyword(s):  
Cell Death ◽  
Nmda Receptor ◽  
Rat Brain ◽  
Neuronal Cell Death ◽  
Neuronal Cell ◽  
Nmda Receptor Antagonists ◽  
Neonatal Rats ◽  
Receptor Antagonists ◽  
Fluoro Jade B ◽  
Hematoxylin And Eosin

Administration of pediatric anesthetics with N-methyl D-aspartate (NMDA)-receptor antagonist and/or γ-aminobutyric acid (GABA) agonist activities may result in neuronal degeneration and/or neuronal cell death in neonatal rats. Evaluating pediatric drug candidates for this potential neurotoxicity is often part of overall preclinical new drug development strategy. This specialized assessment may require dosing neonatal rats at postnatal day 7 at the peak of the brain growth spurt and evaluating brain tissue 24 to 48 hours following dosing. The need to identify methods to aid in the accurate and reproducible detection of lesions associated with this type of neurotoxic profile is paramount for meeting the changing needs of neuropathology assessment and addressing emerging challenges in the neuroscience field. We document the use of Fluoro-Jade B (FJB) staining, to be used in conjunction with standard hematoxylin and eosin staining, to detect acute neurodegeneration and neuronal cell death that can be caused by some NMDA-receptor antagonists and/or GABA agonists in the neonatal rat brain. The FJB staining is simple, specific, and sensitive and can be performed on brain specimens from the same cohort of animals utilized for standard neurotoxicity assessment, thus satisfying animal welfare recommendations with no effect on achievement of scientific and regulatory goals.

Resveratrol ameliorates hypoxia/ischemia-induced behavioral deficits and brain injury in the neonatal rat brain

2011 ◽  
Vol 1425 ◽  
pp. 98-110 ◽  
Author(s):  
Filippos Karalis ◽  
Vassiliki Soubasi ◽  
Thomas Georgiou ◽  
Christos T. Nakas ◽  
Constantina Simeonidou ◽  
...  
Keyword(s):  
Brain Injury ◽  
Rat Brain ◽  
Neonatal Rat ◽  
Behavioral Deficits ◽  
Hypoxia Ischemia ◽  
Neonatal Rat Brain

Neuroprotection against hypoxia-ischemia in neonatal rat brain by novel superoxide dismutase mimetics

2003 ◽  
Vol 346 (1-2) ◽  
pp. 41-44 ◽  
Author(s):  
Katsuyoshi Shimizu ◽  
Nishadi Rajapakse ◽  
Takashi Horiguchi ◽  
R.Mark Payne ◽  
David W. Busija
Keyword(s):  
Superoxide Dismutase ◽  
Rat Brain ◽  
Neonatal Rat ◽  
Hypoxia Ischemia ◽  
Superoxide Dismutase Mimetics ◽  
Neonatal Rat Brain

Hypoxia—ischemia induces heat shock protein-like (HSP72) immunoreactivity in neonatal rat brain

1990 ◽  
Vol 53 (1) ◽  
pp. 145-150 ◽  
Author(s):  
Donna M. Ferriero ◽  
Hernani Q. Soberano ◽  
Roger P. Simon ◽  
Frank R. Sharp
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Rat Brain ◽  
Neonatal Rat ◽  
Hypoxia Ischemia ◽  
Neonatal Rat Brain

Expression of nitric oxide synthase isoforms and nitrotyrosine formation after hypoxia–ischemia in the neonatal rat brain

2005 ◽  
Vol 167 (1-2) ◽  
pp. 64-71 ◽  
Author(s):  
Evelyn R.W. van den Tweel ◽  
Cora Nijboer ◽  
Annemieke Kavelaars ◽  
Cobi J. Heijnen ◽  
Floris Groenendaal ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Rat Brain ◽  
Neonatal Rat ◽  
Hypoxia Ischemia ◽  
Oxide Synthase ◽  
Neonatal Rat Brain

scholarly journals Involvement of apoptosis-inducing factor in neuronal death after hypoxia-ischemia in the neonatal rat brain

2004 ◽  
Vol 86 (2) ◽  
pp. 306-317 ◽  
Author(s):  
Changlian Zhu ◽  
Lin Qiu ◽  
Xiaoyang Wang ◽  
Ulrika Hallin ◽  
Céline Candé ◽  
...  
Keyword(s):  
Rat Brain ◽  
Neuronal Death ◽  
Neonatal Rat ◽  
Hypoxia Ischemia ◽  
Apoptosis Inducing Factor ◽  
Neonatal Rat Brain
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