Effects of the Antiglucocorticoid RU 486 on the Initiation of Ultrastructural Type-II Cell Differentiation in Fetal Rat Lung

Neonatology ◽  
1990 ◽  
Vol 58 (3) ◽  
pp. 173-180 ◽  
Author(s):  
Nadia Guettari ◽  
Marie-Elizabeth Dufour ◽  
Léa Marin
Keyword(s):  
Cell Differentiation ◽  
Type Ii ◽  
Rat Lung ◽  
Ru 486 ◽  
Fetal Rat ◽  
Fetal Rat Lung ◽  
Type Ii Cell

Maturational changes induced by 1 alpha,25-dihydroxyvitamin D3 in type II cells from fetal rat lung explants

1993 ◽  
Vol 265 (1) ◽  
pp. L45-L52 ◽  
Author(s):  
L. Marin ◽  
M. E. Dufour ◽  
T. M. Nguyen ◽  
C. Tordet ◽  
M. Garabedian
Keyword(s):  
Surface Area ◽  
Normal Lung ◽  
Type Ii ◽  
Rat Lung ◽  
Dihydroxyvitamin D3 ◽  
Type Ii Pneumocytes ◽  
Fetal Rat ◽  
Fetal Rat Lung ◽  
Type Ii Cell ◽  
The Mean

Specific binding sites for 1 alpha,25 dihydroxyvitamin D3 [1 alpha,25-(OH)2D3] localized to type II pneumocytes have been evidenced in fetal rat lung at the end of gestation, suggesting a role for vitamin D3 in the control of lung maturation. In this study, we describe the morphological changes that occur in lung explants from 18-day-old rat fetuses grown for 1 and 2 days in control conditions and in the presence of 1 alpha,25(OH)2D3 (10(-9) M) or dexamethasone (10(-7) M). Point counting and planimetric measurements on light and electron micrographs show that 1 alpha,25-(OH)2D3 1) dramatically decreases the mean glycogen content of type II cell profiles between days 1 and 2 of the culture, suggesting an acceleration of the glycogenolytic processes normally occurring at that stage and 2) does not change the intracellular osmiophilic lamellar body (OLB) content of cell profiles, but increases the amount of intraluminal surfactant by 126% when expressed as surfactant clusters surface area/section surface area and by 129% when expressed on a per cell basis, suggesting a stimulation of surfactant synthesis and secretion. By contrast, dexamethasone increases the mean intracellular OLB content of type II cell profiles by 306% and decreases the relative surface area of secreted material by 53 and 73%. In conclusion, 1 alpha,25(OH)2D3 accelerates the physiological maturation of fetal rat type II pneumocytes and could represent a key factor for the onset of normal lung function at birth.

Effects of Cis-Hydroxyproline on Type II Cell Development in Fetal Rat Lung

1987 ◽  
Vol 12 (4) ◽  
pp. 347-362 ◽  
Author(s):  
Gillian M. King ◽  
Ian Y. R. Adamson
Keyword(s):  
Cell Development ◽  
Type Ii ◽  
Rat Lung ◽  
Fetal Rat ◽  
Fetal Rat Lung ◽  
Type Ii Cell

scholarly journals Maternal administration of dexamethasone stimulates choline-phosphate cytidylyltransferase in fetal type II cells

1987 ◽  
Vol 241 (1) ◽  
pp. 291-296 ◽  
Author(s):  
M Post
Keyword(s):  
Enzyme Activity ◽  
Specific Activity ◽  
Active Form ◽  
Type Ii Cells ◽  
Type Ii ◽  
Rat Lung ◽  
Dexamethasone Treatment ◽  
Fetal Rat ◽  
Fetal Rat Lung ◽  
Choline Phosphate

Administration of dexamethasone to pregnant rats at 19 days gestation increased phosphatidylcholine synthesis (45%) from radioactive choline in type II cells. This enhanced synthesis of phosphatidylcholine was accompanied by an increased conversion of choline phosphate into CDP-choline. Similar results were obtained by incubating organotypic cultures of 19-day-fetal rat lung with cortisol. The increased conversion of choline phosphate into CDP-choline correlated with an enhanced choline-phosphate cytidylyltransferase activity (31% after dexamethasone treatment; 47% after cortisol exposure) in the cell homogenates. A similar increase (26% after dexamethasone treatment; 39% after cortisol exposure) was found in the microsomal-associated enzyme. No differences in cytosolic enzyme activity were observed. The specific activity of the microsomal enzyme was 3-4 times that of the cytosolic enzyme. Most of the enzyme activity was located in the microsomal fraction (58-65%). The treatments had no effect on the total amount of enzyme recovered from the cell homogenates. These results, taken collectively, are interpreted to indicate that the active form of cytidylyltransferase in type II cells is the membrane-bound enzyme and that cytidylyltransferase activation in type II cells from fetal rat lung after maternal glucocorticoid administration occurs by binding of inactive cytosolic enzyme to endoplasmic reticulum.

scholarly journals Cigarette Smoke Induces Apoptosis by Activation of Caspase-3 in Isolated Fetal Rat Lung Type II Alveolar Ep-ithelial Cells <i>in Vitro</i>

2013 ◽  
Vol 03 (01) ◽  
pp. 4-12 ◽  
Author(s):  
Asra Ahmed ◽  
James A. Thliveris ◽  
Anthony Shaw ◽  
Michael Sowa ◽  
James Gilchrist ◽  
...  
Keyword(s):  
Cigarette Smoke ◽  
Caspase 3 ◽  
Type Ii ◽  
Rat Lung ◽  
Fetal Rat ◽  
Fetal Rat Lung

Isolation of alveolar type II cells from fetal rat lung by differential adherence in monolayer culture

1988 ◽  
Vol 960 (3) ◽  
pp. 441-453 ◽  
Author(s):  
Joseph J. Batenburg ◽  
Caroline J.M. Otto-Verberne ◽  
Ank A.W. Ten Have-Opbroek ◽  
Wies Klazinga
Keyword(s):  
Monolayer Culture ◽  
Alveolar Type ◽  
Type Ii Cells ◽  
Type Ii ◽  
Rat Lung ◽  
Alveolar Type Ii Cells ◽  
Fetal Rat ◽  
Fetal Rat Lung

scholarly journals The Role of Glutamine and Other Alternate Substrates as Energy Sources in the Fetal Rat Lung Type II Cell1

1996 ◽  
Vol 40 (1) ◽  
pp. 135-141 ◽  
Author(s):  
Renee E Fox ◽  
Irene B Hopkins ◽  
Erwin T Cabacungan ◽  
J Tyson Tildon
Keyword(s):  
Energy Sources ◽  
Type Ii ◽  
Rat Lung ◽  
Fetal Rat ◽  
Fetal Rat Lung
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