Plasma Concentrations of Insulin, Glucose, Free Fatty Acids and Ketone Bodies in the Foetal and Newborn Sheep and the Response to a Glucose Load Before and After Birth

Neonatology ◽  
1969 ◽  
Vol 14 (3-4) ◽  
pp. 178-193 ◽  
Author(s):  
Pauline Alexander ◽  
H.G. Britton ◽  
N.M. Cohen ◽  
D.A. Nixon
Keyword(s):  
Fatty Acids ◽  
Free Fatty Acids ◽  
Ketone Bodies ◽  
Plasma Concentrations ◽  
Glucose Load ◽  
Before And After

Changes in the plasma concentration of gastric inhibitory polypeptide and other metabolites in response to feeding in sheep

1992 ◽  
Vol 134 (2) ◽  
pp. 235-240 ◽  
Author(s):  
J. P. McCarthy ◽  
A. Faulkner ◽  
P. A. Martin ◽  
D. J. Flint
Keyword(s):  
Fatty Acids ◽  
Gastrointestinal Tract ◽  
Plasma Concentration ◽  
Free Fatty Acids ◽  
Plasma Concentrations ◽  
Gastric Inhibitory Polypeptide ◽  
Before And After ◽  
Ruminant Animals ◽  
Time Courses ◽  
Long Chain

ABSTRACT Plasma concentrations of gastric inhibitory polypeptide (GIP)-like activity were determined in sheep before and after refeeding following a 48-h fast. Plasma concentrations increased significantly after feeding, from about 250 pg/ml to about 550 pg/ml. Other metabolites in plasma also increased at this time, reflecting the absorption of nutrients from the gastrointestinal tract. Significant increases were observed in the plasma concentrations of acetate, β-hydroxybutyrate and triacylglycerol. By comparing the time-courses of the changes in concentration of GIP and other metabolites in plasma, possible sites of secretion and secretagogues of GIP in ruminant animals are proposed. The results demonstrate that GIP is secreted in response to nutrient absorption in adult ruminants and that, as in simple-stomached animals, the absorption of long-chain free fatty acids plays an important role in this secretion. Journal of Endocrinology (1992) 134, 235–240

Factors influencing the concentration of minerals and metabolites in the plasma of cattle

1980 ◽  
Vol 94 (2) ◽  
pp. 369-376 ◽  
Author(s):  
G. Wiener ◽  
W. S. Russell ◽  
A. C. Field
Keyword(s):  
Fatty Acids ◽  
Free Fatty Acids ◽  
Milk Yield ◽  
Ketone Bodies ◽  
Plasma Concentrations ◽  
Standard Errors ◽  
Factors Influencing

SummaryOne hundred and seventy-seven female Ayrshire cattle from approximately 6 months to 6 years of age were sampled on one occasion to determine the concentrations in plasma of Cu, P, Ca, Mg, Na, K and glucose. Eighty-nine adults, either lactating or dry, were re-sampled 2 months later to estimate concentrations of ketone bodies and free fatty acids. The data were analysed to estimate the contributions of various factors to variation in the concentrations.The combination of age and stage of pregnancy or lactation significantly affected variation in all the constituents although some of this variation was accounted for by milk yield. The concentrations of Cu, Mg, glucose and ketones were significantly affected by milk yield. The presence of ketone bodies in milk (recorded in relation to the first occasion of bleeding only) was associated with a significant increase in the plasma concentrations of Na and Mg and a significant decrease of K and Ca.The herd comprised pairs of genetically related animals. However, the estimates of heritability had large standard errors and only those for P and Ca were significantly positive.

Integrated analysis of protein and glucose metabolism during surgery: effects of anesthesia

2001 ◽  
Vol 91 (6) ◽  
pp. 2523-2530 ◽  
Author(s):  
Thomas Schricker ◽  
Ralph Lattermann ◽  
Pierre Fiset ◽  
Linda Wykes ◽  
Franco Carli
Keyword(s):  
Fatty Acids ◽  
Glucose Metabolism ◽  
Free Fatty Acids ◽  
Plasma Concentrations ◽  
Glucose Production ◽  
Whole Body ◽  
Anesthetic Technique ◽  
Integrated Analysis ◽  
Body Protein ◽  
Before And After

The aim of this study was to assess dynamic changes in protein and glucose metabolism during surgery. Twelve patients undergoing colorectal surgery received either intravenous propofol anesthesia ( n = 6) or inhalational anesthesia with desflurane ( n = 6). Pre- and intraoperative protein and glucose kinetics were analyzed by an isotope dilution technique usingl-[1-13C]leucine and [6,6-2H2]glucose. Plasma concentrations of glucose, lactate, free fatty acids, insulin, glucagon, and cortisol were measured before and after 2 h of surgery. The rates of appearance of leucine and glucose, leucine oxidation, protein synthesis, and glucose clearance decreased during surgery, independent of the type of anesthesia ( P < 0.05). A correlation between the rate of appearance of leucine and glucose was observed ( r = 0.755, P < 0.001). Intraoperative plasma cortisol and glucose concentrations increased ( P< 0.05), whereas plasma concentrations of lactate, free fatty acids, insulin, and glucagon did not change. Surgery causes a depression of whole body protein and glucose metabolism, independent of the anesthetic technique. There is a correlation between perioperative glucose production and protein breakdown.

Effect of increased plasma free fatty acid concentrations on muscle metabolism in exercising men

1991 ◽  
Vol 70 (1) ◽  
pp. 194-201 ◽  
Author(s):  
M. Hargreaves ◽  
B. Kiens ◽  
E. A. Richter
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Free Fatty Acid ◽  
Free Fatty Acids ◽  
Carbohydrate Metabolism ◽  
Ketone Bodies ◽  
Plasma Concentrations ◽  
Plasma Free Fatty Acid ◽  
Substrate Utilization ◽  
Moderate Intensity

The effect of increasing plasma concentrations of free fatty acids on substrate utilization in muscle during exercise was investigated in 11 healthy young males. One hour of dynamic knee extension at 80% of knee-extensor maximal work capacity was performed first with one leg and then with the other leg during infusion of Intralipid and heparin. Substrate utilization was assessed from arterial and femoral venous blood sampling as well as from muscle biopsies. Intralipid infusion increased mean plasma free fatty acid concentrations from 0.54 +/- 0.08 to 1.12 +/- 0.09 (SE) mM. Thigh glucose uptake during rest, exercise, and recovery was decreased by 64, 33, and 42%, respectively, by Intralipid, whereas muscle glycogen breakdown and release of lactate, pyruvate, and citrate were unaffected. Concentrations of glucose, glucose 6-phosphate, and lactate in muscle before and at termination of exercise were unaffected by Intralipid. During exercise, net leg uptake of plasma free fatty acids was not measurably increased by Intralipid, whereas uptake of ketone bodies was. Local respiratory quotient across the leg was not changed by Intralipid (control 0.87 +/- 0.02, Intralipid 0.86 +/- 0.02). Arterial concentrations of insulin, norepinephrine, and epinephrine were similar in the two trials. It is concluded that at rest and during exercise at a moderate intensity (requiring approximately equal contributions from fat and carbohydrate metabolism), muscle carbohydrate metabolism is affected only with regard to uptake of glucose when plasma concentrations of lipid and lipid metabolites are increased. This effect may be by direct inhibition of glucose transport rather than by the classic glucose-fatty acid cycle.

scholarly journals Effects of 3-hydroxybutyrate and free fatty acids on muscle protein kinetics and signaling during LPS-induced inflammation in humans: anticatabolic impact of ketone bodies

2018 ◽  
Vol 108 (4) ◽  
pp. 857-867 ◽  
Author(s):  
Henrik H Thomsen ◽  
Nikolaj Rittig ◽  
Mogens Johannsen ◽  
Andreas B Møller ◽  
Jens Otto Jørgensen ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Free Fatty Acids ◽  
Acute Inflammation ◽  
Ketone Bodies ◽  
Muscle Protein ◽  
Initiation Factor ◽  
Whole Body ◽  
Protein Breakdown ◽  
Protein Kinetics ◽  
Nonesterified Fatty Acids

Abstract Background Acute inflammation, and subsequent release of bacterial products (e.g. LPS), inflammatory cytokines, and stress hormones, is catabolic, and the loss of lean body mass predicts morbidity and mortality. Lipid intermediates may reduce protein loss, but the roles of free fatty acids (FFAs) and ketone bodies during acute inflammation are unclear. Objective We aimed to test whether infusions of 3-hydroxybutyrate (3OHB), FFAs, and saline reduce protein catabolism during exposure to LPS and Acipimox (to restrict and control endogenous lipolysis). Design A total of 10 healthy male subjects were randomly tested 3 times, with: 1) LPS, Acipimox (Olbetam) and saline, 2) LPS, Acipimox, and nonesterified fatty acids (Intralipid), and 3) LPS, Acipimox, and 3OHB, during a 5-h basal period and a 2-h hyperinsulinemic, euglycemic clamp. Labeled phenylalanine, tyrosine, and urea tracers were used to estimate protein kinetics, and muscle biopsies were taken for Western blot analysis of protein metabolic signaling. Results 3OHB infusion increased 3OHB concentrations (P < 0.0005) to 3.5 mM and decreased whole-body phenylalanine-to-tyrosine degradation. Basal and insulin-stimulated net forearm phenylalanine release decreased by >70% (P < 0.005), with both appearance and phenylalanine disappearance being profoundly decreased. Phosphorylation of eukaryotic initiation factor 2α at Ser51 was increased in skeletal muscle, and S6 kinase phosphorylation at Ser235/236 tended (P = 0.074) to be decreased with 3OHB infusion (suggesting inhibition of protein synthesis), whereas no detectable effects were seen on markers of protein breakdown. Lipid infusion did not affect phenylalanine kinetics, and insulin sensitivity was unaffected by interventions. Conclusion During acute inflammation, 3OHB has potent anticatabolic actions in muscle and at the whole-body level; in muscle, reduction of protein breakdown overrides inhibition of synthesis. This trial was registered at clinicaltrials.gov as NCT01752348.

Influence of High Plasma Concentrations of Free Fatty Acids on Heart Rhythm in Healthy Fasting Men

2009 ◽  
Vol 205 (1-6) ◽  
pp. 299-301 ◽  
Author(s):  
Harald Wang ◽  
Knut Rasmussen ◽  
Harald Vik-Mo ◽  
Ole D. Mjøs ◽  
Helge Grendahl
Keyword(s):  
Fatty Acids ◽  
Free Fatty Acids ◽  
Plasma Concentrations ◽  
Heart Rhythm ◽  
High Plasma

The response of plasma minerals, free fatty acids and glucose to adrenaline and cortisol infusion in sheep

1986 ◽  
Vol 106 (2) ◽  
pp. 209-217 ◽  
Author(s):  
Sarah C. Bolton ◽  
T. E. C. Weekes
Keyword(s):  
Fatty Acids ◽  
Free Fatty Acids ◽  
Marked Decrease ◽  
Plasma Concentrations ◽  
Sodium Concentration ◽  
Plasma Calcium ◽  
Plasma Sodium ◽  
Plasma Phosphate ◽  
Plasma Sodium Concentration ◽  
Plasma Magnesium

SUMMARYAdrenaline was infused at three rates, 40, 15 or 3 μ/kg/h, in normal sheep and in sheep rendered hypercortisolaemic by infusion of cortisol at 150 μg/kg/h. In both normal and hypercortisolaemic animals, plasma concentrations of glucose and free fatty acids were increased by adrenaline treatment; plasma phosphate decreased with all treatments; plasma magnesium and potassium decreased on infusion of adrenaline at 40 or 15, but not at 3 μg/kg/h; plasma calcium decreased only on infusion of adrenaline in hypercortisolaemic animals, and plasma sodium concentration was unaffected by treatment.Induction of a degree of lipolysis likely to occur in the field was not associated with a marked decrease in plasma magnesium.

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