Substitution of an Aspartic Acid Results in Constitutive Activation of c-kitReceptor Tyrosine Kinase in a Rat Tumor Mast Cell Line RBL-2H3

1995 ◽  
Vol 106 (4) ◽  
pp. 377-385 ◽  
Author(s):  
Tohru Tsujimura ◽  
Takuma Furitsu ◽  
Masahiro Morimoto ◽  
Yoshio Kanayama ◽  
Shintaro Nomura ◽  
...  
Keyword(s):  
Mast Cell ◽  
Tyrosine Kinase ◽  
Cell Line ◽  
Aspartic Acid ◽  
Receptor Tyrosine Kinase ◽  
Constitutive Activation ◽  
Mast Cell Line ◽  
Rat Tumor

scholarly journals Transcriptional complexity of the HSPG2 gene in the human mast cell line, HMC-1

2014 ◽  
Vol 35 ◽  
pp. 123-131 ◽  
Author(s):  
Megan S. Lord ◽  
MoonSun Jung ◽  
Bill Cheng ◽  
John M. Whitelock
Keyword(s):  
Mast Cell ◽  
Cell Line ◽  
Human Mast Cell ◽  
Mast Cell Line

The lysosomal protease cathepsin D is efficiently sorted to and secreted from regulated secretory compartments in the rat basophilic/mast cell line RBL

2000 ◽  
Vol 113 (18) ◽  
pp. 3289-3298 ◽  
Author(s):  
A. Dragonetti ◽  
M. Baldassarre ◽  
R. Castino ◽  
M. Demoz ◽  
A. Luini ◽  
...  
Keyword(s):  
Mast Cell ◽  
Mast Cells ◽  
Cell Line ◽  
Cathepsin D ◽  
Secretory Granules ◽  
Bioactive Molecules ◽  
Blue Staining ◽  
Lysosomal Protease ◽  
Mannose 6 Phosphate ◽  
Mast Cell Line

Basophils and mast cells contain a peculiar class of inflammatory granules that discharge their content upon antigen-mediated crosslinking of IgE-membrane receptors. The pathways for granule biogenesis and exocytosis in these cells are still largely obscure. In this study we employed the rat basophilic leukemia (RBL)/mast cell line to verify the hypothesis that inflammatory granules share common bioactive molecules and functional properties with lysosomes. We demonstrate that inflammatory granules, as identified by the monoclonal 5G10 antibody (which recognises an integral membrane protein) or by Toluidine Blue staining, have an intralumenal acidic pH, possess lysosomal enzymes and are accessible by fluid-phase and membrane endocytosis markers. In addition, we studied the targeting, subcellular localisation and regulated secretion of the lysosomal aspartic protease cathepsin D (CD) as affected by IgE receptor stimulation in order to obtain information on the pathways for granule biogenesis and exocytosis. Stimulation with DNP-BSA of specific IgE-primed RBL cells led to a prompt release of processed forms of CD, along with other mature lysosomal hydrolases. This release could be prevented by addition of EGTA, indicating that it was dependent on extracellular calcium influx. Antigen stimulation also induced exocytosis of immature CD forms accumulated by ammonium chloride, suggesting the existence of an intermediate station in the pathway for granule biogenesis still sensitive to regulated exocytosis. The targeting of molecules to secretory granules may occur via either a mannose-6-phosphate-dependent or mannose-6-phosphate-independent pathway. We conclude that endosomes and lysosomes in basophils/mast cells can act as regulated secretory granules or actually identify with them.

Tenidap: a novel inhibitor of calcium influx in a mast cell line

Cell Calcium ◽  
1993 ◽  
Vol 14 (1) ◽  
pp. 1-16 ◽  
Author(s):  
P.L. Cleveland ◽  
P.J. Millard ◽  
H.J. Showell ◽  
C.M.S. Fewtrell
Keyword(s):  
Mast Cell ◽  
Cell Line ◽  
Calcium Influx ◽  
Mast Cell Line
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