Murine Granulocyte-Macrophage and Mast Cell Colony Formation Promoted by Nerve Growth Factor

1993 ◽  
Vol 102 (4) ◽  
pp. 362-367 ◽  
Author(s):  
Yukiko Kannan ◽  
Hiroshi Matsuda ◽  
Hiroko Ushio ◽  
Keiko Kawamoto ◽  
Yasuaki Shimada
Keyword(s):  
Mast Cell ◽  
Nerve Growth Factor ◽  
Growth Factor ◽  
Colony Formation ◽  
Nerve Growth ◽  
Cell Colony

Nerve growth factor levels and mast cell distribution in human coronary atherosclerosis

2001 ◽  
Vol 159 (1) ◽  
pp. 57-66 ◽  
Author(s):  
George N. Chaldakov ◽  
Ivan S. Stankulov ◽  
Marco Fiore ◽  
Peter I. Ghenev ◽  
Luigi Aloe
Keyword(s):  
Mast Cell ◽  
Nerve Growth Factor ◽  
Growth Factor ◽  
Coronary Atherosclerosis ◽  
Cell Distribution ◽  
Nerve Growth

Effects of nerve growth factor antagonist K252a on peritoneal mast cell degranulation: implications for rat postoperative ileus

2015 ◽  
Vol 309 (10) ◽  
pp. G801-G806 ◽  
Author(s):  
Sergio Berdún ◽  
Jakub Rychter ◽  
Patri Vergara
Keyword(s):  
Mast Cell ◽  
Nerve Growth Factor ◽  
Growth Factor ◽  
Postoperative Ileus ◽  
Peritoneal Mast Cell ◽  
In Vivo Model ◽  
Dependent Manner ◽  
Nerve Growth

Stabilization of mast cell (MC) degranulation has been proposed to prevent postoperative ileus (POI). Nerve growth factor (NGF) mediates MC degranulation. The aim of the study was to evaluate whether NGF receptor antagonist K252a acts as a MC stabilizer in vitro and in vivo model of POI. Peritoneal mast cells (PMCs) were obtained from Sprague-Dawley rats and were incubated with K252a and exposed to NGF or Compound 48/80 (C48/80). MC degranulation was assessed by β-hexosaminidase assay. POI was induced in rats by intestinal manipulation (IM). Rats were pretreated with K252a (100 μg/kg sc) 20 min prior to POI induction. At 20 min after IM, release of rat mast cell protease 6 (RMCP-6) was evaluated in peritoneal lavage. At 24 h, intestinal transit (IT) and gastric emptying (GE) were evaluated. Ileal inflammation was assessed by myeloperoxidase (MPO) activity, expression of IL-6, NGF, TrkA, RMCP-2 and 6, and MC density within the full-thickness ileum. C48/80 and NGF evoked degranulation of PMCs in a dose-dependent manner. K252a prevented NGF-evoked, but not C48/80-evoked, MC degranulation. IM evoked the release of peritoneal RMCP-6 and subsequently delayed IT and GE. IM increased MPO activity and expression of IL-6. In IM rats, K252a prevented upregulation of IL-6 expression and reduced TrkA. IT, GE, and inflammation were not affected by K252a. K252a inhibited NGF-evoked degranulation of PMCs in vitro. In vivo, K252a decreased IL-6 and PMC degranulation. This may be of relevance for the development of new therapeutic targets for POI.

scholarly journals The level of nerve growth factor and its relationship with the content of leukocytes and mast cells in experimental interstitial cystitis/painful bladder syndrome

2019 ◽  
Vol 9 (3) ◽  
pp. 5-11
Author(s):  
Rashad Farhad ogly Sholan
Keyword(s):  
Mast Cell ◽  
Nerve Growth Factor ◽  
Growth Factor ◽  
Mast Cells ◽  
Interstitial Cystitis ◽  
Cell Infiltration ◽  
Bladder Tissue ◽  
Nerve Growth ◽  
Painful Bladder ◽  
Group 2

Relevance. Interstitial cystitis/painful bladder syndrome (IC/PBS) is a debilitating condition of pain and discomfort in the bladder. Due to the limited number of published studies in this area, there is a need for further research. Objective: to evaluate the level of nerve growth factor and its relationship with various types of leukocytes and mast cells of the bladder tissue in animals with experimental models of interstitial cystitis/painful bladder syndrome. Material and methods. IC/PBS modeling was performed on 38 female rabbits. The IC/PBS modeling was created by introducing HCl into the bladder (group 1), urine into the wall of the bladder (group 2), and physiological saline (group 3). Nerve growth factor (NGF) was determined by the ELISA method, and white blood cells along with mast cells in tissues were determined by histological processing. Results. A statistically significant high level of NGF in blood and urine was observed on the 1st day after IC/PBS initiation in groups 13 and in group 2 respectively. Two weeks after the initiation of IC/PBS in animals of group 1 a decrease in the level of FRN in the blood by 29.3% and its increase in urine by 14.3% was observed; in group 2 an increase by 65.5% (p 0.01) in blood and by 52.7% (p 0.01) in urine was observed and in group 3 a decrease by 30.8% (p 0.05) in blood and by 30.5% (p 0.05) in urine was observed. The greatest number of leukocytes was determined in the tissue of the bladder in animals of the 1st group. Mast cell infiltration was observed in groups 1 and 2. Correlation between the NGF in blood and urine and between NGF and the number of leukocytes and mast cells was revealed. Findings. In IC/PBS, the level of NGF in the blood and urine is increased. Indicators of nerve growth factor in blood and urine are correlated with multidirectional connections. High mast cell infiltration occurs when damage to the integrity of the bladder by urinary toxicity. Nerve growth factor correlates with leukocyte and mast cell infiltration in the bladder tissue. Conclusion. During IC/PBS, the level of NGF in the blood and urine is increased. Levels of nerve growth factor in blood and urine are correlated with multidirectional connections. High mast cell infiltration occurs as a response to the damage of the bladder wall integrity caused by urinary toxicity. The level of nerve growth factor correlates with the bladder tissue intensity of infiltration with leukocytes and mast cells.

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