DNA Typing with Digoxigenin-11-Dideoxyuridinetriphosphate-Labelled Oligonucleotide Probes Enables Non-Radioactive Analysis Using a Dual Detection System: Application for Screening HLA-DQA1 Polymorphisms

1993 ◽  
Vol 101 (1) ◽  
pp. 7-12 ◽  
Author(s):  
Hans-Peter Rihs ◽  
Andreas Thiele ◽  
Bruno Perichon ◽  
Rajagopal Krishnamoorthy ◽  
Xaver Baur
Keyword(s):  
System Application ◽  
Detection System ◽  
Dna Typing ◽  
Oligonucleotide Probes ◽  
Dual Detection ◽  
Hla Dqa1

Rapid DNA typing utilizing immobilized oligonucleotide probe and a nonradioactive detection system application to HLA-DR typing of the Japanese population

1992 ◽  
Vol 154 (2) ◽  
pp. 205-210 ◽  
Author(s):  
Akio Abe ◽  
Mihoko Kitagawa ◽  
Yuriko Ikuta ◽  
Shoichi Miyakoshi ◽  
Hirofumi Danbara ◽  
...  
Keyword(s):  
System Application ◽  
Japanese Population ◽  
Oligonucleotide Probe ◽  
Detection System ◽  
Dna Typing ◽  
Hla Dr

scholarly journals Genotyping of 27 Human Papillomavirus Types by Using L1 Consensus PCR Products by a Single-Hybridization, Reverse Line Blot Detection Method

1998 ◽  
Vol 36 (10) ◽  
pp. 3020-3027 ◽  
Author(s):  
P. E. Gravitt ◽  
C. L. Peyton ◽  
R. J. Apple ◽  
C. M. Wheeler
Keyword(s):  
Human Papillomavirus ◽  
Detection System ◽  
Sampling Error ◽  
Blot Hybridization ◽  
High Sensitivity ◽  
Oligonucleotide Probes ◽  
Dot Blot ◽  
Consensus Primer ◽  
Hpv Dna ◽  
Silver Spring

Amplification of human papillomavirus (HPV) DNA by L1 consensus primer systems (e.g., MY09/11 or GP5+/6+) can detect as few as 10 to 100 molecules of HPV targets from a genital sample. However, genotype determination by dot blot hybridization is laborious and requires at least 27 separate hybridizations for substantive HPV-type discrimination. A reverse blot method was developed which employs a biotin-labeled PCR product hybridized to an array of immobilized oligonucleotide probes. By the reverse blot strip analysis, genotype discrimination of multiple HPV types can be accomplished in a single hybridization and wash cycle. Twenty-seven HPV probe mixes, two control probe concentrations, and a single reference line were immobilized to 75- by 6-mm nylon strips. Each individual probe line contained a mixture of two bovine serum albumin-conjugated oligonucleotide probes specific to a unique HPV genotype. The genotype spectrum discriminated on this strip includes the high-risk, or cancer-associated, HPV genotypes 16, 18, 26, 31, 33, 35, 39, 45, 51, 52, 55, 56, 58, 59, 68 (ME180), MM4 (W13B), MM7 (P291), and MM9 (P238A) and the low-risk, or non-cancer-associated, genotypes 6, 11, 40, 42, 53, 54, 57, 66, and MM8 (P155). In addition, two concentrations of β-globin probes allowed for assessment of individual specimen adequacy following amplification. We have evaluated the performance of the strip method relative to that of a previously reported dot blot format (H. M. Bauer et al., p. 132–152, in C. S. Herrington and J. O. D. McGee (ed.), Diagnostic Molecular Pathology: a Practical Approach, (1992), by testing 328 cervical swab samples collected in Digene specimen transport medium (Digene Diagnostics, Silver Spring, Md.). We show excellent agreement between the two detection formats, with 92% concordance for HPV positivity (kappa = 0.78, P < 0.001). Nearly all of the discrepant HPV-positive samples resulted from weak signals and can be attributed to sampling error from specimens with low concentrations (<1 copy/μl) of HPV DNA. The primary advantage of the strip-based detection system is the ability to rapidly genotype HPVs present in genital samples with high sensitivity and specificity, minimizing the likelihood of misclassification.

Comparison of HLA class II alleles in Gypsy and Czech populations by DNA typing with oligonucleotide probes

1992 ◽  
Vol 39 (3) ◽  
pp. 111-116 ◽  
Author(s):  
Marie Cerná ◽  
Marcelo Fernandez-Viña ◽  
Eva Ivásková ◽  
Peter Stastny
Keyword(s):  
Dna Typing ◽  
Class Ii ◽  
Hla Class Ii ◽  
Oligonucleotide Probes ◽  
Hla Class Ii Alleles

DNA typing of DRw6 subtypes: Correlation with DRB1 and DRB3 allelic sequences by hybridization with oligonucleotide probes

1989 ◽  
Vol 24 (1) ◽  
pp. 1-14 ◽  
Author(s):  
Jean-Marie Tiercy ◽  
Jack Gorski ◽  
Hervé Bétuel ◽  
A. Catherine Freidel ◽  
Lucette Gebuhrer ◽  
...  
Keyword(s):  
Dna Typing ◽  
Oligonucleotide Probes

scholarly journals Evidence for a primary association of celiac disease to a particular HLA-DQ alpha/beta heterodimer.

1989 ◽  
Vol 169 (1) ◽  
pp. 345-350 ◽  
Author(s):  
L M Sollid ◽  
G Markussen ◽  
J Ek ◽  
H Gjerde ◽  
F Vartdal ◽  
...  
Keyword(s):  
Celiac Disease ◽  
Oligonucleotide Probes ◽  
Hla Dq ◽  
Allele Specific ◽  
In Trans ◽  
Dqb1 Gene ◽  
Alpha Beta ◽  
Hla Dqa1 ◽  
In Cis ◽  
Dqb1 Allele

Typing of DNA from 94 unrelated children with celiac disease (CD) with HLA-DQA1 and -DQB1 allele-specific oligonucleotide probes revealed that all but one (i.e., 98.9%) may share a particular combination of a DQA1 and a DQB1 gene. These genes are arranged in cis position on the DR3DQw2 haplotype and in trans position in DR5DQw7/DR7DQw2 heterozygous individuals. Thus, most CD patients may share the same cis- or trans-encoded HLA-DQ alpha/beta heterodimer.

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