Ultrastructural Morphology of Human Mast Cell Progenitors in Sequential Cocultures of Cord Blood Cells and Fibroblasts

1993 ◽  
Vol 100 (3) ◽  
pp. 219-229 ◽  
Author(s):  
Ann M. Dvorak ◽  
Takuma Furitsu ◽  
Teruko Ishizaka
Keyword(s):  
Mast Cell ◽  
Cord Blood ◽  
Blood Cells ◽  
Human Mast Cell ◽  
Ultrastructural Morphology ◽  
Cord Blood Cells

Interleukin-6 Directly Modulates Stem Cell Factor-Dependent Development of Human Mast Cells Derived From CD34+Cord Blood Cells

Blood ◽  
1999 ◽  
Vol 94 (2) ◽  
pp. 496-508 ◽  
Author(s):  
Tatsuya Kinoshita ◽  
Nobukuni Sawai ◽  
Eiko Hidaka ◽  
Tetsuji Yamashita ◽  
Kenichi Koike
Keyword(s):  
Mast Cell ◽  
Stem Cell ◽  
Mast Cells ◽  
Cord Blood ◽  
Interleukin 6 ◽  
Blood Cells ◽  
Stem Cell Factor ◽  
Flow Cytometric Analysis ◽  
Flow Cytometric ◽  
Cord Blood Cells

In the present study, we attempted to clarify the effects of interleukin-6 (IL-6) on the growth and properties of human mast cells using cultured mast cells selectively generated by stem cell factor (SCF) from CD34+ cord blood cells. The addition of IL-6 to cultures containing mast cells resulted in a substantial reduction of the number of progenies grown by SCF in the liquid culture. This IL-6–mediated inhibition of mast cell growth may be due in part to the suppression at the precursor level, according to the results of a clonal cell culture assay. Moreover, a flow cytometric analysis showed that the cultured mast cells grown in the presence of SCF+IL-6 had decreased c-kit expression. The exposure of cultured mast cells to SCF+IL-6 also caused substantial increases in the cell size, frequency of chymase-positive cells, and intracellular histamine level compared with the values obtained with SCF alone. The flow cytometric analysis showed low but significant levels of expression of IL-6 receptor (IL-6R) and gp130 on the cultured mast cells grown with SCF. The addition of either anti–IL-6R antibody or anti-gp130 antibody abrogated the biological functions of IL-6. Although IL-4 exerted an effect similar to that of IL-6 on the cultured mast cells under stimulation with SCF, the results of comparative experiments suggest that the two cytokines use different regulatory mechanisms. Taken together, the present findings suggest that IL-6 modulates SCF-dependent human mast cell development directly via an IL-6R-gp130 system.

Interleukin-6 Directly Modulates Stem Cell Factor-Dependent Development of Human Mast Cells Derived From CD34+Cord Blood Cells

Blood ◽  
1999 ◽  
Vol 94 (2) ◽  
pp. 496-508 ◽  
Author(s):  
Tatsuya Kinoshita ◽  
Nobukuni Sawai ◽  
Eiko Hidaka ◽  
Tetsuji Yamashita ◽  
Kenichi Koike
Keyword(s):  
Mast Cell ◽  
Stem Cell ◽  
Mast Cells ◽  
Cord Blood ◽  
Interleukin 6 ◽  
Blood Cells ◽  
Stem Cell Factor ◽  
Flow Cytometric Analysis ◽  
Flow Cytometric ◽  
Cord Blood Cells

Abstract In the present study, we attempted to clarify the effects of interleukin-6 (IL-6) on the growth and properties of human mast cells using cultured mast cells selectively generated by stem cell factor (SCF) from CD34+ cord blood cells. The addition of IL-6 to cultures containing mast cells resulted in a substantial reduction of the number of progenies grown by SCF in the liquid culture. This IL-6–mediated inhibition of mast cell growth may be due in part to the suppression at the precursor level, according to the results of a clonal cell culture assay. Moreover, a flow cytometric analysis showed that the cultured mast cells grown in the presence of SCF+IL-6 had decreased c-kit expression. The exposure of cultured mast cells to SCF+IL-6 also caused substantial increases in the cell size, frequency of chymase-positive cells, and intracellular histamine level compared with the values obtained with SCF alone. The flow cytometric analysis showed low but significant levels of expression of IL-6 receptor (IL-6R) and gp130 on the cultured mast cells grown with SCF. The addition of either anti–IL-6R antibody or anti-gp130 antibody abrogated the biological functions of IL-6. Although IL-4 exerted an effect similar to that of IL-6 on the cultured mast cells under stimulation with SCF, the results of comparative experiments suggest that the two cytokines use different regulatory mechanisms. Taken together, the present findings suggest that IL-6 modulates SCF-dependent human mast cell development directly via an IL-6R-gp130 system.

Long-term engraftment stability of umbilical cord blood cells preserved in −135 °C mechanical freezers

2021 ◽  
pp. 106590
Author(s):  
Harumi Kato ◽  
Hirofumi Taji ◽  
Takeshi Kodaira ◽  
Tomohiro Kinoshita ◽  
Kazuhito Yamamoto
Keyword(s):  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Blood Cells ◽  
Cord Blood Cells ◽  
Umbilical Cord Blood Cells

In Utero Transplantation of Human Cord Blood Cells into Rabbits

2005 ◽  
Vol 80 (2) ◽  
pp. 282-283 ◽  
Author(s):  
Georg S. Wengler ◽  
Guerino Lombardi ◽  
Tiziana Frusca ◽  
Daniele Alberti ◽  
Alberto Albertini ◽  
...  
Keyword(s):  
Cord Blood ◽  
Blood Cells ◽  
In Utero ◽  
Human Cord Blood ◽  
In Utero Transplantation ◽  
Cord Blood Cells

scholarly journals Ultrastructural Studies of Human Basophils and Mast Cells

2005 ◽  
Vol 53 (9) ◽  
pp. 1043-1070 ◽  
Author(s):  
Ann M. Dvorak
Keyword(s):  
Growth Factor ◽  
Mast Cells ◽  
Cord Blood ◽  
Blood Cells ◽  
De Novo ◽  
Ultrastructural Studies ◽  
Specific Growth Factor ◽  
Cord Blood Cells ◽  
Human Basophils

Ultrastructural studies of human mast cells (HMCs) and basophils (HBs) are reviewed. Sources of HMCs include biopsies of tissue sites and in situ study of excised diseased organs; isolated, partially purified samples from excised organs; and growth-factor-stimulated mast cells that develop de novo in cultures of cord blood cells. Sources of HBs for study include partially purified peripheral blood basophils, basophils in tissue biopsies, and specific growth factor-stimulated basophils arising de novo from cord blood cells. The ultrastructural studies reviewed deal with identity, secretion, vesicles, recovery, and synthesis issues related to the biology of these similar cells.

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