Legionella pneumophila – Host Interactions: Insights Gained from Comparative Genomics and Cell Biology

2009 ◽  
pp. 170-186 ◽  
Author(s):  
M. Lomma ◽  
Gomez Valero ◽  
C. Rusniok ◽  
C. Buchrieser
Keyword(s):  
Comparative Genomics ◽  
Legionella Pneumophila ◽  
Cell Biology ◽  
Host Interactions

scholarly journals The Pathogen-Host Interactions Database (PHI-base) Provides Insights into Generic and Novel Themes of Pathogenicity

2006 ◽  
Vol 19 (12) ◽  
pp. 1451-1462 ◽  
Author(s):  
Thomas K. Baldwin ◽  
Rainer Winnenburg ◽  
Martin Urban ◽  
Chris Rawlings ◽  
Jacob Koehler ◽  
...  
Keyword(s):  
Comparative Genomics ◽  
Virulence Genes ◽  
Host Responses ◽  
Plant Responses ◽  
Avirulence Genes ◽  
Pathogenicity Genes ◽  
Host Interactions ◽  
Effector Genes ◽  
Computational Resources

Fungal and oomycete pathogens of plants and animals are a major global problem. In the last 15 years, many genes required for pathogenesis have been determined for over 50 different species. Other studies have characterized effector genes (previously termed avirulence genes) required to activate host responses. By studying these types of pathogen genes, novel targets for control can be revealed. In this report, we describe the Pathogen-Host Interactions database (PHI-base), which systematically compiles such pathogenicity genes involved in pathogen-host interactions. Here, we focus on the biology that underlies this computational resource: the nature of pathogen-host interactions, the experimental methods that exist for the characterization of such pathogen-host interactions as well as the available computational resources. Based on the data, we review and analyze the specific functions of pathogenicity genes, the host-specific nature of pathogenicity and virulence genes, and the generic mechanisms of effectors that trigger plant responses. We further discuss the utilization of PHI-base for the computational identification of pathogenicity genes through comparative genomics. In this context, the importance of standardizing pathogenicity assays as well as integrating databases to aid comparative genomics is discussed.

scholarly journals Complete Genome Sequences of Six Legionella pneumophila Isolates from Two Collocated Outbreaks of Legionnaires’ Disease in 2005 and 2008 in Sarpsborg/Fredrikstad, Norway

2016 ◽  
Vol 4 (6) ◽  
Author(s):  
Marius Dybwad ◽  
Tone Aarskaug ◽  
Else-Marie Fykse ◽  
Elisabeth Henie Madslien ◽  
Janet Martha Blatny
Keyword(s):  
Comparative Genomics ◽  
Genetic Relationship ◽  
Legionella Pneumophila ◽  
Complete Genome ◽  
Sequence Similarity ◽  
Secretion Systems ◽  
Environmental Isolates ◽  
Genome Sequences ◽  
High Sequence Similarity ◽  
Legionnaires Disease

Here, we report the complete genome sequences of Legionella pneumophila isolates from two collocated outbreaks of Legionnaires’ disease in 2005 and 2008 in Sarpsborg/Fredrikstad, Norway. One clinical and two environmental isolates were sequenced from each outbreak. The genome of all six isolates consisted of a 3.36 Mb-chromosome, while the 2005 genomes featured an additional 68 kb-episome sharing high sequence similarity with the L. pneumophila Lens plasmid. All six genomes contained multiple mobile genetic elements including novel combinations of type-IVA secretion systems. A comparative genomics study will be launched to resolve the genetic relationship between the L. pneumophila isolates.

Molecular and cell biology of Legionella pneumophila

2004 ◽  
Vol 293 (7-8) ◽  
pp. 519-527 ◽  
Author(s):  
Dina M. Bitar ◽  
Maëlle Molmeret ◽  
Yousef Abu Kwaik
Keyword(s):  
Legionella Pneumophila ◽  
Cell Biology

scholarly journals Corynebacterium Comparative Genomics Reveals a Role for Cobamide Sharing in the Skin Microbiome

2020 ◽  
Author(s):  
Mary Hannah Swaney ◽  
Lindsay R Kalan
Keyword(s):  
Comparative Genomics ◽  
Microbial Communities ◽  
Community Dynamics ◽  
De Novo ◽  
Skin Barrier ◽  
Skin Microbiome ◽  
Bacterial Phyla ◽  
Host Interactions ◽  
Complex Interactions ◽  
Associated Species

ABSTRACTThe human skin microbiome is a key player in human health, with diverse functions ranging from defense against pathogens to education of the immune system. Recent studies have begun unraveling the complex interactions within skin microbial communities, shedding light on the invaluable role that skin microorganisms have in maintaining a healthy skin barrier. While the Corynebacterium genus is a dominant taxon of the skin microbiome, relatively little is known how skin-associated Corynebacteria contribute to microbe-microbe and microbe-host interactions on the skin. Here, we performed a comparative genomics analysis of 71 Corynebacterium species from diverse ecosystems, which revealed functional differences between host- and environment-associated species. In particular, host-associated species were enriched for de novo biosynthesis of cobamides, which are a class of cofactor essential for metabolism in organisms across the tree of life but are produced by a limited number of prokaryotes. Because cobamides have been hypothesized to mediate community dynamics within microbial communities, we analyzed skin metagenomes for Corynebacterium cobamide producers, which revealed a positive correlation between cobamide producer abundance and microbiome diversity, a trait associated with skin health. We also provide the first metagenome-based assessment of cobamide biosynthesis and utilization in the skin microbiome, showing that both dominant and low abundant skin taxa encode for the de novo biosynthesis pathway and that cobamide-dependent enzymes are encoded by phylogenetically diverse taxa across the major bacterial phyla on the skin. Taken together, our results support a role for cobamide sharing within skin microbial communities, which we hypothesize mediates community dynamics.

scholarly journals Cellular Electron Cryo-Tomography to Study Virus-Host Interactions

2020 ◽  
Vol 7 (1) ◽  
pp. 239-262
Author(s):  
Emmanuelle R.J. Quemin ◽  
Emily A. Machala ◽  
Benjamin Vollmer ◽  
Vojtěch Pražák ◽  
Daven Vasishtan ◽  
...  
Keyword(s):  
Viral Entry ◽  
Cell Biology ◽  
Molecular Mechanisms ◽  
Virus Assembly ◽  
Three Dimensional ◽  
Surface Proteins ◽  
Host Cells ◽  
Phase Plate ◽  
Structural Cell ◽  
Host Interactions

Viruses are obligatory intracellular parasites that reprogram host cells upon infection to produce viral progeny. Here, we review recent structural insights into virus-host interactions in bacteria, archaea, and eukaryotes unveiled by cellular electron cryo-tomography (cryoET). This advanced three-dimensional imaging technique of vitreous samples in near-native state has matured over the past two decades and proven powerful in revealing molecular mechanisms underlying viral replication. Initial studies were restricted to cell peripheries and typically focused on early infection steps, analyzing surface proteins and viral entry. Recent developments including cryo-thinning techniques, phase-plate imaging, and correlative approaches have been instrumental in also targeting rare events inside infected cells. When combined with advances in dedicated image analyses and processing methods, details of virus assembly and egress at (sub)nanometer resolution were uncovered. Altogether, we provide a historical and technical perspective and discuss future directions and impacts of cryoET for integrative structural cell biology analyses of viruses.

Cell biology of Legionella pneumophila

1999 ◽  
Vol 2 (1) ◽  
pp. 30-34 ◽  
Author(s):  
Joseph P Vogel ◽  
Ralph R Isberg
Keyword(s):  
Legionella Pneumophila ◽  
Cell Biology

The Genetics and Cell Biology ofWolbachia-Host Interactions

2008 ◽  
Vol 42 (1) ◽  
pp. 683-707 ◽  
Author(s):  
Laura R. Serbus ◽  
Catharina Casper-Lindley ◽  
Frédéric Landmann ◽  
William Sullivan
Keyword(s):  
Cell Biology ◽  
Host Interactions

scholarly journals Resolving the homology—function relationship through comparative genomics of membrane-trafficking machinery and parasite cell biology

2016 ◽  
Vol 209 (1-2) ◽  
pp. 88-103 ◽  
Author(s):  
Christen M. Klinger ◽  
Inmaculada Ramirez-Macias ◽  
Emily K. Herman ◽  
Aaron P. Turkewitz ◽  
Mark C. Field ◽  
...  
Keyword(s):  
Comparative Genomics ◽  
Membrane Trafficking ◽  
Cell Biology ◽  
Parasite Cell ◽  
Function Relationship

Cell biology of the intracellular infection by Legionella pneumophila

2004 ◽  
Vol 6 (1) ◽  
pp. 129-139 ◽  
Author(s):  
Maëlle Molmeret ◽  
Dina M. Bitar ◽  
Lihui Han ◽  
Yousef Abu Kwaik
Keyword(s):  
Legionella Pneumophila ◽  
Cell Biology ◽  
Intracellular Infection
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