Production of Eosinophil-Activating Factor (EAF) by Peripheral Blood Mononuclear Cells from Asthma Patients

1989 ◽  
Vol 90 (4) ◽  
pp. 345-351 ◽  
Author(s):  
Kareen J.I. Thorne ◽  
B.A. Richardson ◽  
A.E. Butterworth ◽  
I. Hay ◽  
M. Jackson ◽  
...  
Keyword(s):  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells ◽  
Asthma Patients

scholarly journals 2221. Chlamydia pneumoniae (Cpn) Induces IFN-γ Responses in Peripheral Blood Mononuclear Cells (PBMC) from Pediatric and Adult Asthma Patients: Effects of Age and Inhaled Corticosteroid Use

2019 ◽  
Vol 6 (Supplement_2) ◽  
pp. S758-S758
Author(s):  
Aviva Szigeti ◽  
Margaret Hammerschlag ◽  
Diana Weaver ◽  
Tamar Smith-Norowitz ◽  
Stephan Kohlhoff
Keyword(s):  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Chlamydia Pneumoniae ◽  
Mononuclear Cells ◽  
Inhaled Corticosteroid ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells ◽  
Asthma Patients ◽  
Ifn Γ

Abstract Background Chlamydia pneumoniae (Cpn) is unique in its ability to cause chronic infections, potentially triggering asthma exacerbations as well as subsequent asthma development. Th1-mediated immunity and IFN-γ are critical for clearing chlamydial infections. Persistent or recent Cpn infection may be identified in vitro by detecting T-helper cytokine IFN-γ produced by peripheral blood mononuclear cells (PBMC) stimulated by Cpn. Inhaled corticosteroids (ICS) may have an inhibitory effect on IFN-γ. Prior studies have shown increased Th2 responses upon in vitro Cpn stimulation with increased age. Our aim was to determine whether age and inhaled corticosteroid (ICS) use affect Cpn-induced PBMC produced IFN-γ levels. Methods Pediatric and adult subjects with (n = 23) and without (n = 10) asthma were enrolled. PBMC obtained from all subjects were stimulated with Cpn (MOI = 0.1 x48h) in vitro. IFN-γ levels in culture supernatants were determined by ELISA and reported as pg/mL. Nasopharyngeal (NP) swabs were tested for Cpn using Real-Time PCR. Statistical analysis for continuous variables was performed using the Mann–Whitney U test. Results None of the subjects were positive for Cpn by PCR on NP swab. Levels of IFN-γ produced by PBMC stimulated by Cpn were similar between asthmatic vs. control subjects (41.7 vs. 68.8, respectively; P = 0.72) and between pediatric and adult subjects with asthma (IFN-γ 54 vs. 20.1 respectively, P = 0.95). Pediatric subjects with asthma who received ICS had lower IFN-γ levels than those who did not (median IFN-γ 25.5 vs. 209; P = 0.003). Conclusion Our finding of lower IFN-γ levels among asthma patients on ICS compared with those not on ICS suggests that ICS use may dampen the systemic inflammatory response. While we did not find a statistically significant difference between pediatric and adult age groups in this pilot study, there was a trend to higher Cpn-induced IFN-γ levels among younger pediatric subjects. Future prospective studies should further define predictors of diminished IFN-γ responses in patients with asthma. Disclosures All authors: No reported disclosures.

Interferon-gamma signaling is downregulated in peripheral blood mononuclear cells from asthma patients

2020 ◽  
Vol 4 (2) ◽  
Author(s):  
Carolina Valadares Nunes ◽  
Laila Rigolin Fortunato ◽  
Jhony Robison Oliveira ◽  
Aline Beatriz Mahler Pereira ◽  
Gabriela Rister Figueiredo Irie ◽  
...  
Keyword(s):  
Interferon Gamma ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells ◽  
Asthma Patients

Hyperoxidized peroxiredoxins in peripheral blood mononuclear cells of asthma patients is associated with asthma severity

Life Sciences ◽  
2012 ◽  
Vol 90 (13-14) ◽  
pp. 502-508 ◽  
Author(s):  
Hyouk-Soo Kwon ◽  
Yun-Jeong Bae ◽  
Keun-Ai Moon ◽  
Yoon Su Lee ◽  
Taehoon Lee ◽  
...  
Keyword(s):  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Asthma Severity ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells ◽  
Asthma Patients

Interferon-gamma signaling is downregulated in peripheral blood mononuclear cells from asthma patients

2020 ◽  
Vol 4 (2) ◽  
Author(s):  
Carolina Valadares Nunes ◽  
Laila Rigolin Fortunato ◽  
Jhony Robison Oliveira ◽  
Aline Beatriz Mahler Pereira ◽  
Gabriela Rister Figueiredo Irie ◽  
...  
Keyword(s):  
Interferon Gamma ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells ◽  
Asthma Patients
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