Polymorphonuclear Leukocyte Stimulation Measured by Phage Inactivation

1989 ◽  
Vol 90 (3) ◽  
pp. 207-212 ◽  
Author(s):  
U. Ferrini ◽  
A.M. Mileo ◽  
A. Nista ◽  
E. Mattei ◽  
A. Orofino
Keyword(s):  
Polymorphonuclear Leukocyte ◽  
Phage Inactivation

Platelet Metabolism during the Interiorization of Two Different Types of Particulate Matter

1972 ◽  
Vol 27 (02) ◽  
pp. 263-271 ◽  
Author(s):  
I. A. Cooper ◽  
P Cochrane ◽  
B. G. Firkin ◽  
K. J. Pinkard
Keyword(s):  
Particulate Matter ◽  
Polymorphonuclear Leukocyte ◽  
Anaerobic Metabolism ◽  
Human Platelets ◽  
Metabolic Changes ◽  
Polystyrene Latex ◽  
Considerable Time ◽  
Difference Of Opinion ◽  
Different Types ◽  
Aerobic And Anaerobic

SummaryIt has been suggested that human platelets possess the ability to phagocytose particulate matter similar to the polymorphonuclear leukocyte. However some difference of opinion has arisen regarding this contention, particularly as differences have been demonstrated with regard to the observed metabolic changes occurring in platelets related to such a process.The experiments reported in this paper were designed to observe the aerobic and anaerobic metabolism in human platelets during and following interiorization of two different particles, viz. polystyrene latex and thorotrast. The results of these experiments show a marked difference between both types of particles with regard to observable metabolic changes despite the rapid interiorization of both types of material. Some alteration occurs in both aerobic and anaerobic metabolism a considerable time after interiorization of latex, whereas no alteration could be demonstrated after interiorization of thorotrast. It is suggested that the interiorization of particulate matter is by some process other than phagocytosis and that observed metabolic changes related to latex may be due to a release reaction.

Polymorphonuclear Leukocyte Function in Bacterial and Viral Infections

1982 ◽  
Vol 14 (1) ◽  
pp. 11-18 ◽  
Author(s):  
Claus O. Solberg ◽  
Tore Kalager ◽  
Harry R. Hill ◽  
Johan Gleite
Keyword(s):  
Polymorphonuclear Leukocyte ◽  
Viral Infections ◽  
Leukocyte Function

scholarly journals Endothelial Cell Cortactin Phosphorylation by Src Contributes to Polymorphonuclear Leukocyte Transmigration In Vitro

2006 ◽  
Vol 98 (3) ◽  
pp. 394-402 ◽  
Author(s):  
Lin Yang ◽  
Jennifer R. Kowalski ◽  
Xi Zhan ◽  
Sheila M. Thomas ◽  
Francis W. Luscinskas
Keyword(s):  
Endothelial Cell ◽  
Polymorphonuclear Leukocyte ◽  
Leukocyte Transmigration

Hypoxemia regulates effect of lipopolysaccharide on polymorphonuclear leukocyte CD11b/CD18 expression

1994 ◽  
Vol 76 (4) ◽  
pp. 1657-1663 ◽  
Author(s):  
H. H. Simms ◽  
R. D′Amico
Keyword(s):  
Monoclonal Antibody ◽  
Polymorphonuclear Leukocyte ◽  
Whole Blood ◽  
Lipid A ◽  
Cytochalasin B ◽  
Actinomycin D ◽  
H2o2 Production ◽  
Functional Capability ◽  
Receptor Shedding ◽  
Do So

Expression of the receptor CD11b/CD18 on the polymorphonuclear leukocyte (PMN) surface is important for several aspects of PMN function during endotoxemia. The mechanisms underlying regulation of CD11b/CD18 expression during hypoxemia and endotoxemia, however, are less clear. We investigated the effects of exposure of whole blood PMNs to lipopolysaccharide (LPS) during hypoxemia. During hypoxemia (10–30% O2 saturation), LPS reduced CD11b/CD18 expression. Both kinetic assays and experiments with microfilament stabilizers (phalloidin, cytochalasin B) demonstrated that this was most likely due to receptor shedding. Incubation of whole blood PMNs with an anti-CD14 monoclonal antibody (MEM18) largely prevented the LPS-induced reduction of CD11b/CD18 expression. Decreased CD11b/CD18 expression reduced PMN functional capability, as the binding of its ligand (erythrocytes opsonized with the 3rd component of complement Cbi) and intracellular H2O2 production were reduced. After exposure to LPS, N-formyl-methionyl-leucyl-phenylalanine could rapidly induce new CD11b/CD18 receptors to the cell surface, and this was not inhibited by actinomycin D or cycloheximide. After reoxygenation (> 90% O2 saturation), CD11b/CD18 expression was restored, and this was abrogated by exposure to cytochalasin B. Lipid A was able to reproduce the effects of LPS during hypoxemia and hypoxemia-reoxygenation but required a 10-fold greater concentration to do so. These results demonstrate that during hypoxemia an important pathophysiological property of LPS is to reduce CD11b/CD18 expression. This results in diminished PMN functional capability, which would contribute to the pathogenicity of LPS during acute infectious states.

Beditine, a new benzodioxane derivative, as a suppressor of human polymorphonuclear leukocyte and platelet activation

1997 ◽  
Vol 53 (11) ◽  
pp. 1753-1755 ◽  
Author(s):  
Alexander Panossian ◽  
Gagik Grigorian ◽  
Sergey Akopov ◽  
Anahit Aivasian ◽  
Edward Markarian ◽  
...  
Keyword(s):  
Platelet Activation ◽  
Polymorphonuclear Leukocyte ◽  
Human Polymorphonuclear Leukocyte

scholarly journals Fcα R expression on polymorphonuclear leukocyte and superoxide generation in IgA nephropathy

1994 ◽  
Vol 45 (3) ◽  
pp. 868-875 ◽  
Author(s):  
Abul Kashem ◽  
Masayuki Endoh ◽  
Yasuo Nomoto ◽  
Hideto Sakai ◽  
Hiroe Nakazawa
Keyword(s):  
Iga Nephropathy ◽  
Polymorphonuclear Leukocyte ◽  
Superoxide Generation

Polymorphonuclear leukocyte cells and elastase in tears

1997 ◽  
Vol 16 (8) ◽  
pp. 810-819 ◽  
Author(s):  
Miki Sakata ◽  
Robert A. Sack ◽  
Sonal Sathe ◽  
Brien Holden ◽  
Ann R. Beaton
Keyword(s):  
Polymorphonuclear Leukocyte
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