In vitro Proliferation of Murine Spleen Cells

1989 ◽  
Vol 90 (2) ◽  
pp. 162-168 ◽  
Author(s):  
P. Zhou ◽  
L.J. Quackenbush ◽  
M.B. Zaleski
Keyword(s):  
Spleen Cells ◽  
In Vitro Proliferation ◽  
Murine Spleen

In Vitro Proliferation of Murine Spleen Cells: I. Strain Variation of Response to Medium from Cultures of EL-4 Cells

1989 ◽  
Vol 18 (8) ◽  
pp. 1007-1017 ◽  
Author(s):  
W. K. Dowjat ◽  
P. Zhou ◽  
L. J. Quackenbush ◽  
T. Gorzynski ◽  
M. B. Zoleski
Keyword(s):  
Strain Variation ◽  
Spleen Cells ◽  
In Vitro Proliferation ◽  
Murine Spleen

scholarly journals Mycobacterial Di-O-Acyl-Trehalose Inhibits Mitogen- and Antigen-Induced Proliferation of Murine T Cells In Vitro

2001 ◽  
Vol 8 (6) ◽  
pp. 1081-1088 ◽  
Author(s):  
Rafael Saavedra ◽  
Erika Segura ◽  
Rosario Leyva ◽  
Luis A. Esparza ◽  
Luz M. López-Marı́n
Keyword(s):  
Cell Division ◽  
Cell Envelope ◽  
Inhibitory Effect ◽  
Host Cells ◽  
Mouse Spleen ◽  
Dependent Manner ◽  
Spleen Cells ◽  
Murine Spleen ◽  
Focus Of Infection

ABSTRACT 2,3-Di-O-acyl-trehalose (DAT) is a glycolipid located on the outer layer of the Mycobacterium tuberculosis cell envelope. Due to its noncovalent linkage to the mycobacterial peptidoglycan, DAT could easily interact with host cells located in the focus of infection. The aim of the present work was to study the effects of DAT on the proliferation of murine spleen cells. DAT was purified from reference strains of M. tuberculosis,or M. fortuitum as a surrogate source of the compound, by various chromatography and solvent extraction procedures and then chemically identified. Incubation of mouse spleen cells with DAT inhibited in a dose-dependent manner concanavalin A-stimulated proliferation of the cells. Experiments, including the propidium iodide exclusion test, showed that these effects were not due to death of the cells. Tracking of cell division by labeling with 5,6-carboxyfluorescein diacetate succinimidyl ester revealed that DAT reduces the rounds of cell division. Immunofluorescence with an anti-CD3 monoclonal antibody indicated that T lymphocytes were the population affected in our model. Our experiments also suggest that the extent of the suppressive activity is strongly dependent on the structural composition of the acyl moieties in DATs. Finally, the inhibitory effect was also observed on antigen-induced proliferation of mouse spleen cells specific for Toxoplasma gondii. All of these data suggest that DAT could have a role in the T-cell hyporesponsiveness observed in chronic tuberculosis.

scholarly journals In vitro generation of tumor-specific cytotoxic lymphocytes. Secondary allogeneic mixed tumor lymphocyte culture of normal murine spleen cells

1977 ◽  
Vol 146 (2) ◽  
pp. 468-482 ◽  
Author(s):  
S Gillis ◽  
KA Smith
Keyword(s):  
Cell Surface ◽  
Lymphocyte Culture ◽  
Leukemia Cells ◽  
Cytotoxic Lymphocytes ◽  
Mixed Tumor ◽  
Spleen Cells ◽  
Syngeneic Tumor ◽  
Murine Spleen ◽  
Murine Leukemia

In vivo or in vitro immunity to murine leukemia virus (MuLV)-induced leukemia cells which do not effectively produce virus, has been difficult to demonstrate. Because immunizations with allogeneic murine leukemia cells have been used to confer syngeneic tumor immunity to virus- producing cells, we attempted to generate lymphocytes, cytotoxic to syngeneic nonproducer leukemia cells, by stimulating normal murine spleen cells with allogeneic nonproducer leukemia cells in mixed tumor lymphocyte culture (MTLC) reactions in vitro. Secondary allogeneic MTLC of normal C57BL/6 or DBA/2 spleen cells effectively produced syngeneic tumor-specific cytotoxic lymphocytes. Target cells lysed in lymphocyte- mediated cytolysis (LMC) assays, included both Friend and Rauscher virus- induced syngeneic murine leukemia cells and chemically-induced hematopoietic tumor cells. Syngeneic tumor cells were lysed regardless of whether they produced infectious MuLV or expressed viral antigens gp-71, p-30, or p-12 at the cell surface. Syngeneic normal cells (thymus, lymph node, or Concanavalin A-stimulated spleen cells) used as targets in LMC assays were uneffected by lymphocytes harvested from secondary allogeneic MTLC. Several other in vitro culture treatments including secondary syngeneic MTLC and repetitive mixed lymphocyte culture stimulations were incapable of generating tumor-specific cytotoxic lymphocytes. Based upon these results, we propose that secondary MTLC stimulation of normal spleen cells with allogeneic nonproducer leukemia cells selects for the proliferation of two subpopulations of antigen-specific cytotoxic lymphocytes. The population capable of effecting syngeneic tumor cell lysis is directed against tumor-associated cell surface antigens which may be distinct from viral structural proteins or glycoproteins. The growth of these tumor-specific cytotoxic lymphocytes may be enhanced by a soluble allogeneic effect factor produced by the proliferation of the second subpopulation of lymphocytes generated in repetitive allogeneic MTLC, namely those lymphocytes with specificities directed against differing histocompatibility antigens.

In Vitro Effect of Acetaldehyde on Cell-Mediated Cytotoxicity by Murine Spleen Cells

1989 ◽  
Vol 13 (6) ◽  
pp. 766-771 ◽  
Author(s):  
Amrik S. Walia ◽  
Kenneth M. Pruitt ◽  
Dirck L. Dillehay ◽  
G. M. Marshall ◽  
E. W. Lamon
Keyword(s):  
Spleen Cells ◽  
Murine Spleen ◽  
Vitro Effect

scholarly journals Obligatory role of gamma interferon in T cell-replacing factor-dependent, antigen-specific murine B cell responses.

1985 ◽  
Vol 161 (5) ◽  
pp. 953-971 ◽  
Author(s):  
M Brunswick ◽  
P Lake
Keyword(s):  
T Cell ◽  
B Cell ◽  
Cell Activation ◽  
Human Peripheral Blood ◽  
Gamma Interferon ◽  
Spleen Cells ◽  
Ifn Gamma ◽  
Murine Spleen

The role of gamma interferon (IFN-gamma) in T cell-replacing factor (TRF) activity for antigen-specific plaque-forming cell (PFC) responses in vitro was studied using antibodies to murine IFN-gamma (Mu IFN-gamma). TRF activity was present in supernatants (Sn) of Con A- or mixed leukocyte reaction-stimulated murine spleen cells as well as in an IL-2-rich fraction of phytohemagglutinin-stimulated human peripheral blood lymphocyte Sn and in the Sn of the Gibbon T lymphoma MLA-144. The human TRF was highly active with cells from nu/nu mice and normal mice but not with cells from animals with the xid immunologic defect, similar to the activity of murine TRF. Antibodies to IFN-gamma consisted of hyper-immune rabbit antisera, IFN-gamma affinity-purified rabbit immunoglobulin and an interspecies hybridoma specific for Mu IFN-gamma. The results show that the activities of all preparations of TRF are markedly diminished or abrogated by antibody to Mu IFN-gamma but not by antibodies to human IFN-gamma (Hu IFN-gamma), nor by normal rabbit sera or purified rabbit Ig. The degree of inhibition was dose dependent and was quantitatively reversed by the addition to the cultures of recombinant-derived Mu IFN-gamma (Mu rIFN-gamma) but not Hu rIFN-gamma. This reversal was fully antigen specific and thus not attributable to polyclonal B cell activation by IFN-gamma, which is inactive alone in the TRF assay. Kinetic analysis shows that IFN-gamma must act by 24-48 h to produce PFC responses at 4 d. Together, the data demonstrate that IFN-gamma is a necessary mediator for TRF effects and that IFN-gamma is induced by TRF from T-depleted murine spleen cells in sufficient quantity to support large antibody responses. The source of this IFN-gamma may be the potent natural killer cells that are induced in cultures stimulated with TRF.

Production of IgG-producing hybridomas by in vitro stimulation of murine spleen cells

1987 ◽  
Vol 96 (2) ◽  
pp. 247-253 ◽  
Author(s):  
Miyoko Takahashi ◽  
Steven A. Fuller ◽  
John G.R. Hurrell
Keyword(s):  
Spleen Cells ◽  
Murine Spleen ◽  
Stimulation Of
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