Effects of Calcium lonophore and Phorbol Ester on Class-II-Restricted Virus-Specific Human Cytotoxic T Cell Clones

1988 ◽  
Vol 87 (2) ◽  
pp. 178-183
Author(s):  
John R. Richert ◽  
Eve D. Robinson
Keyword(s):  
T Cell ◽  
Phorbol Ester ◽  
Class Ii ◽  
Cytotoxic T Cell ◽  
T Cell Clones ◽  
Cell Clones

scholarly journals Specific killing of cytotoxic T cells and antigen-presenting cells by CD4+ cytotoxic T cell clones. A novel potentially immunoregulatory T-T cell interaction in man.

1990 ◽  
Vol 171 (6) ◽  
pp. 2011-2024 ◽  
Author(s):  
T H Ottenhoff ◽  
T Mutis
Keyword(s):  
T Cells ◽  
T Cell ◽  
Class Ii ◽  
Cytolytic Activity ◽  
Hla Class Ii ◽  
Cytotoxic T Cell ◽  
Target Antigen ◽  
T Cell Clones ◽  
Cell Clones ◽  
Hla Dq

Mycobacterial antigens not only stimulate Th cells that produce macrophage-activating factors, but also CD4+ and CD8+ CTL that lyse human macrophages. The mycobacterial recombinant 65-kD hsp was previously found to be an important target antigen for polyclonal CD4+ CTL. Because of the major role of 65-kD hsp in the immune response to mycobacterial as well as autoantigens, we have studied CTL activity to this protein at the clonal level. HLA-DR or HLA-DQ restricted, CD4+CD8- T cell clones that recognize different peptides of the M. leprae 65-kD hsp strongly lysed EBV-BLCL pulsed with specific but not irrelevant peptide. No bystander lysis of B cells, T cells, or tumor cells was seen. Target cell lysis could not be triggered by PMA + Ca2+ ionophore alone and depended on active metabolism. Interestingly, these CD4+ CTL also strongly lysed themselves and other HLA-class II compatible CD4+ (TCR-alpha/beta or -gamma/delta) or CD8+ CTL clones in the presence of peptide, suggesting that CTL are not actively protected from CTL-mediated lysis. Cold target competition experiments suggested that EBV-BLCL targets were more efficiently recognized than CD4+ CTL targets. These results demonstrate that hsp65 peptide-specific HLA class II-restricted CD4+ T cell clones display strong peptide-dependent cytolytic activity towards both APCs, and, unexpectedly, CD4+ and CD8+ CTL clones, including themselves. Since, in contrast to murine T cells human T cells express class II, CTL-mediated T cell killing may represent a novel immunoregulatory pathway in man.

HLA Class II Alloantigens Defined by Cytotoxic T Cell Clones and Monoclonal Antibodies

1984 ◽  
pp. 495-499 ◽  
Author(s):  
N. Flomenberg ◽  
K. Horibe ◽  
R. W. Knowles ◽  
D. Williams ◽  
K. Rosenkrantz ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
T Cell ◽  
Class Ii ◽  
Hla Class Ii ◽  
Cytotoxic T Cell ◽  
T Cell Clones ◽  
Cell Clones

scholarly journals Self-sparing of long-term in vitro-cloned or uncloned cytotoxic T lymphocytes.

1986 ◽  
Vol 164 (3) ◽  
pp. 962-967 ◽  
Author(s):  
M F Luciani ◽  
J F Brunet ◽  
M Suzan ◽  
F Denizot ◽  
P Golstein
Keyword(s):  
T Cell ◽  
T Lymphocytes ◽  
Cytotoxic T Lymphocytes ◽  
Cytotoxic T Cell ◽  
Cell Populations ◽  
Con A ◽  
T Cell Clones ◽  
Cell Clones

At least some long-term in vitro-cultured cytotoxic T cell clones and uncloned cell populations are able, in the presence of Con A, to lyse other cells, to be lysed by other cells, but not to lyse themselves. This as-yet-unexplained result may have implications as to the mechanism of T cell-mediated cytotoxicity.

Xenogeneic cytotoxic T-cell clones recognize alloantigenic determinants on HLA-A2

1983 ◽  
Vol 18 (5) ◽  
pp. 461-473 ◽  
Author(s):  
Victor H. Engelhard ◽  
Christopher Benjamin
Keyword(s):  
T Cell ◽  
Cytotoxic T Cell ◽  
T Cell Clones ◽  
Cell Clones

Transfected murine cells expressing HLA class II can be used to generate alloreactive human T cell clones

1994 ◽  
Vol 169 (1) ◽  
pp. 25-33 ◽  
Author(s):  
Anthony N. Warrens ◽  
Tricia Heaton ◽  
Sid Sidhu ◽  
Giovanna Lombardi ◽  
Robert I. Lechler
Keyword(s):  
T Cell ◽  
Class Ii ◽  
Hla Class Ii ◽  
T Cell Clones ◽  
Human T Cell ◽  
Cell Clones

scholarly journals Recognition of envelope protein by dengue virus serotype-specific human CD4+ CD8- cytotoxic T-cell clones.

1994 ◽  
Vol 68 (5) ◽  
pp. 3283-3288 ◽  
Author(s):  
P G Livingston ◽  
I Kurane ◽  
C J Lai ◽  
M Bray ◽  
F A Ennis
Keyword(s):  
T Cell ◽  
Dengue Virus ◽  
Envelope Protein ◽  
Cytotoxic T Cell ◽  
T Cell Clones ◽  
Cell Clones ◽  
Virus Serotype
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