Characterization of Trypanosoma cruzi Antigens Recognized by Sera from Patients with Chronic Chagas’ Disease

1987 ◽  
Vol 84 (4) ◽  
pp. 410-413 ◽  
Author(s):  
S. Gea ◽  
P. Rodriguez ◽  
E. Vottero-Cima
Keyword(s):  
Trypanosoma Cruzi ◽  
Chagas Disease ◽  
Chronic Chagas Disease

scholarly journals Genetic Characterization of Trypanosoma cruzi Directly from Tissues of Patients with Chronic Chagas Disease

2000 ◽  
Vol 156 (5) ◽  
pp. 1805-1809 ◽  
Author(s):  
Annamaria R. Vago ◽  
Luciana O. Andrade ◽  
Adriana A. Leite ◽  
Débora d'Ávila Reis ◽  
Andrea M. Macedo ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Chagas Disease ◽  
Genetic Characterization ◽  
Chronic Chagas Disease

scholarly journals Evolution of anti-Trypanosoma cruzi antibody production in patients with chronic Chagas disease: Correlation between antibody titers and development of cardiac disease severity

2017 ◽  
Vol 11 (7) ◽  
pp. e0005796 ◽  
Author(s):  
Ingebourg Georg ◽  
Alejandro Marcel Hasslocher-Moreno ◽  
Sergio Salles Xavier ◽  
Marcelo Teixeira de Holanda ◽  
Eric Henrique Roma ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Disease Severity ◽  
Chagas Disease ◽  
Antibody Production ◽  
Cardiac Disease ◽  
Antibody Titers ◽  
Chronic Chagas Disease

scholarly journals Correction: Trypanosoma cruzi-specific IFN-γ-producing cells in chronic Chagas disease associate with a functional IL-7/IL-7R axis

2019 ◽  
Vol 13 (2) ◽  
pp. e0007168 ◽  
Author(s):  
María A. Natale ◽  
Gonzalo Cesar ◽  
Maria G. Alvarez ◽  
Melisa D. Castro Eiro ◽  
Bruno Lococo ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Chagas Disease ◽  
Ifn Γ ◽  
Chronic Chagas Disease

scholarly journals Characterization of Trypanosoma cruzi Sirtuins as Possible Drug Targets for Chagas Disease

2015 ◽  
Vol 59 (8) ◽  
pp. 4669-4679 ◽  
Author(s):  
Nilmar Silvio Moretti ◽  
Leonardo da Silva Augusto ◽  
Tatiana Mordente Clemente ◽  
Raysa Paes Pinto Antunes ◽  
Nobuko Yoshida ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Chagas Disease ◽  
Drug Targets ◽  
Wild Type ◽  
Content Type ◽  
Damage Repair ◽  
Lysine Deacetylases

ABSTRACTAcetylation of lysine is a major posttranslational modification of proteins and is catalyzed by lysine acetyltransferases, while lysine deacetylases remove acetyl groups. Among the deacetylases, the sirtuins are NAD+-dependent enzymes, which modulate gene silencing, DNA damage repair, and several metabolic processes. As sirtuin-specific inhibitors have been proposed as drugs for inhibiting the proliferation of tumor cells, in this study, we investigated the role of these inhibitors in the growth and differentiation ofTrypanosoma cruzi, the agent of Chagas disease. We found that the use of salermide during parasite infection prevented growth and initial multiplication after mammalian cell invasion byT. cruziat concentrations that did not affect host cell viability. In addition,in vivoinfection was partially controlled upon administration of salermide. There are two sirtuins inT. cruzi, TcSir2rp1 and TcSir2rp3. By using specific antibodies and cell lines overexpressing the tagged versions of these enzymes, we found that TcSir2rp1 is localized in the cytosol and TcSir2rp3 in the mitochondrion. TcSir2rp1 overexpression acts to impair parasite growth and differentiation, whereas the wild-type version of TcSir2rp3 and not an enzyme mutated in the active site improves both. The effects observed with TcSir2rp3 were fully reverted by adding salermide, which inhibited TcSir2rp3 expressed inEscherichia coliwith a 50% inhibitory concentration (IC50) ± standard error of 1 ± 0.5 μM. We concluded that sirtuin inhibitors targeting TcSir2rp3 could be used in Chagas disease chemotherapy.

scholarly journals Molecular characterization of Trypanosoma cruzi Mexican strains and their behavior in the mouse experimental model

2011 ◽  
Vol 44 (6) ◽  
pp. 684-690 ◽  
Author(s):  
César Gómez-Hernández ◽  
Karine Rezende-Oliveira ◽  
Gabriel Antônio Nogueira Nascentes ◽  
Lara Rocha Batista ◽  
Henrique Borges Kappel ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Chagas Disease ◽  
Random Amplified Polymorphic Dna ◽  
Geographic Origin ◽  
Pcr Amplification ◽  
Biological Characterization ◽  
Long Time ◽  
Intergenic Regions ◽  
Mexican Strains

INTRODUCTION: For a long time, the importance of Chagas disease in Mexico, where many regarded it as an exotic malady, was questioned. Considering the great genetic diversity among isolates of Trypanosoma cruzi, the importance of this biological characterization, and the paucity of information on the clinical and biological aspects of Chagas disease in Mexico, this study aimed to identify the molecular and biological characterization of Trypanosoma cruzi isolates from different endemic areas of this country, especially of the State of Jalisco. METHODS: Eight Mexican Trypanosoma cruzi strains were biologically and genetically characterized (PCR specific for Trypanosoma cruzi, multiplex-PCR, amplification of space no transcript of the genes of the mini-exon, amplification of polymorphic regions of the mini-exon, classification by amplification of intergenic regions of the spliced leader genes, RAPD - (random amplified polymorphic DNA). RESULTS: Two profiles of parasitaemia were observed, patent (peak parasitaemia of 4.6×10(6) to 10(7) parasites/mL) and subpatent. In addition, all isolates were able to infect 100% of the animals. The isolates mainly displayed tropism for striated (cardiac and skeletal) muscle. PCR amplification of the mini-exon gene classified the eight strains as TcI. The RAPD technique revealed intraspecies variation among isolates, distinguishing strains isolated from humans and triatomines and according to geographic origin. CONCLUSIONS: The Mexican T. cruzi strains are myotrophic and belong to group TcI.

Functionally active cardiac antibodies in chronic Chagas' disease are specifically blocked by Trypanosoma cruzi antigens

1998 ◽  
Vol 12 (14) ◽  
pp. 1551-1558 ◽  
Author(s):  
Masako Oya Masuda ◽  
Mariano Levin ◽  
Selma Farias De Oliveira ◽  
Patricia C. Dos Santos Costa ◽  
Pablo Lopez Bergami ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Chagas Disease ◽  
Chronic Chagas Disease

scholarly journals Trypanosoma cruzi and experimental Chagas' disease: characterization of a stock isolated from a patient with associated digestive and cardiac form

1993 ◽  
Vol 26 (1) ◽  
pp. 25-33 ◽  
Author(s):  
E.C. Oliveira ◽  
M.M.A. Stefani ◽  
A.O. Luquetti ◽  
E.F. Vêncio ◽  
M.A.R. Moreira ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Digestive Tract ◽  
Chagas Disease ◽  
Acute Phase ◽  
Experimental Infection ◽  
Post Infection ◽  
Chronic Phase ◽  
Igg Antibodies ◽  
Histopathology Examination

A new Trypanosoma cruzi stock isolated from a patient in the chronic phase of Chagas' disease with the digestive and cardiac fortn of the disease was characterized by experimental infection in isogenic, susceptible, A/Sn strain mice. Parasitemia curves showed up to 1.7x10(6) parasites/ml and no mortality was observed up to 300 days post infection. Specific IgM was found in mice in the acute phase up to 40 days and also in the chronic phase. IgG antibodies yvere detected in the acute and chronic phase. Histopathology examination demonstrated myotropism to the digestive tract muscle layers and to the heart.

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