Fractionation of Human Thymocytes and Peripheral Blood Lymphocytes on Percoll Density Gradients and DNA Synthesis Stimulating Effect of Mercuric Chloride

1984 ◽  
Vol 75 (1) ◽  
pp. 16-19 ◽  
Author(s):  
K. Nordlind
Keyword(s):  
Dna Synthesis ◽  
Mercuric Chloride ◽  
Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
Density Gradients ◽  
Blood Lymphocytes

Antigenotoxic effect of melatonin against mercuric chloride in human peripheral blood lymphocytes

2018 ◽  
Vol 34 (11) ◽  
pp. 778-786 ◽  
Author(s):  
Tapan A Patel ◽  
Mandava V Rao
Keyword(s):  
Mercuric Chloride ◽  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Antioxidant Property ◽  
Peripheral Blood Lymphocytes ◽  
Blood Cultures ◽  
High Concentration ◽  
Blood Lymphocytes ◽  
Antigenotoxic Effect

Melatonin (MLT) is an extraordinary antioxidant, which plays an important role in reducing reactive oxygen species (ROS) by scavenging them directly or indirectly. Mercury (Hg) is a heavy metal, which induces cytogenetic alterations via various mechanisms, leading to genotoxicity. It induces genotoxicity by enhancing the ROS chiefly. In the present study, the antigenotoxic effect of MLT was evaluated against mercuric chloride (HgCl2). All experiments were conducted in vitro in peripheral blood lymphocytes. Blood cultures were exposed to various concentrations of HgCl2 (2.63, 6.57, and 10.52 microM) for 24 h to study a range of genotoxic parameters. MLT (0.2 mM) supplementation, alone and in combination with the high concentration of Hg, was administered to blood cultures for 24 h. Genotoxic parameters, such as chromosomal aberrations (CAs; structural aberrations (chromatid gaps and breaks, chromosomal gaps and breaks) and numerical aberrations), micronuclei (MNs), and comet assay, were evaluated and analyzed using suitable statistical analysis. Hg treatment revealed a significant increase in CAs, MNs, and comet length. Co-supplementation of MLT along with Hg showed marked protection of these genotoxic end points in treated cultures. In conclusion, our findings suggest that MLT protects against Hg-induced augmentation in genotoxic indices because of its antioxidant property.

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