Relationship between Mitogenic Factor and in vitro Lymphocyte Stimulation during Guinea Pig Immune Response to KLH

1977 ◽  
Vol 54 (4) ◽  
pp. 347-355 ◽  
Author(s):  
L.A.E. Ashworth
Keyword(s):  
Immune Response ◽  
Guinea Pig ◽  
Lymphocyte Stimulation ◽  
Mitogenic Factor

scholarly journals THE SPECIFICITY OF ALLERGIC REACTIONS

1963 ◽  
Vol 117 (3) ◽  
pp. 401-423 ◽  
Author(s):  
John E. Coe ◽  
S. B. Salvin
Keyword(s):  
Immune Response ◽  
Guinea Pig ◽  
The State ◽  
Delayed Hypersensitivity ◽  
Protein Carrier ◽  
Specific Contact ◽  
Tolerant Animal ◽  
Gastric Feeding

"Gastric feeding" of adult guinea pigs with dinitrochlorobenzene (DCB) resulted in a specific unresponsiveness to sensitization with the specific contact hapten. The more DCB gastric-fed to a guinea pig, the more complete the unresponsiveness to the hapten. When mycobacteria were incorporated into the sensitizing emulsion, the state of unresponsiveness to the dinitrophenyl (DNP) group was less apparent. When animals gastric-fed with DCB were later sensitized with an in vitro conjugate of the hapten combined with a heterologous protein such as dinitrophenyl-hen egg albumin (DNP·HEA), an immune response similar to that in the controls occurred both to the hapten and to the protein carrier. However, when the tolerant animals were sensitized with a conjugate containing a homologous protein carrier such as dinitrophenyl guinea pig serum (DNP·GPS), they showed diminished immune responses in comparison with those in the non-tolerant controls. The presence of circulating anti-DNP antibodies from sensitization with DNP-HEA did not affect the unresponsiveness to the specific contact hapten, regardless of whether these antibodies are present before or after induction of tolerance. Sensitization with picryl chloride (PiCl) (a cross-reacting hapten), either before or after gastric feeding of DCB, did not affect the state of unresponsiveness to DNP. Similarly when the DNP-tolerant animal was sensitized with PiCl, the subsequent immune response was similar to that in the controls; cross-reactions with the DNP group both in the contact and circulating antibody phase occurred at a rate similar to that in the controls. The foregoing relationships can be explained by presuming that, upon the gastric feeding of DCB, an in vivo conjugate is formed with a somatic protein, which determines the basic specificity of the tolerance. Acquired tolerance seems to manifest an immunologic specificity similar to that of delayed hypersensitivity, a relationship not unexpected if delayed hypersensitivity is an early phase of the immune response.

scholarly journals Whole-blood lymphocyte stimulation assay for measurement of cell-mediated immune responses in bovine brucellosis

1978 ◽  
Vol 7 (6) ◽  
pp. 550-557
Author(s):  
J M Kaneene ◽  
R K Anderson ◽  
D W Johnson ◽  
C C Muscoplat ◽  
P Nicoletti ◽  
...  
Keyword(s):  
Immune Response ◽  
Whole Blood ◽  
Blood Lymphocyte ◽  
Lymphocyte Stimulation ◽  
Soluble Antigen ◽  
Bovine Brucellosis ◽  
Serological Tests ◽  
Cell Mediated Immune Response ◽  
Stimulation Tests

A study was conducted to develop an in vitro whole-blood lymphocyte stimulation assay for measurement of cell-mediated immune response in bovine brucellosis. A soluble antigen (BASA) prepared from killed cells of Brucella abortus 1119-3 was used. Cattle infected with B. abortus field strains, B. abortus 19 calfhood- and adult-vaccinated cattle, and nonexposed cattle were tested. Blood was diluted 10-fold in RPMI-1640 medium (without added serum) and cultured with BASA (at a concentration of 2.2 microgram per culture) at varying times of incubation. Results were assayed for [3H]thymidine incorporation into deoxyribonucleic acid. A 6-day period was found to be optimal for incubating blood cultures to achieve maximum specific lymphocyte stimulation. Serological tests and bacteriological isolation attempts were conducted simultaneously with lymphocyte stimulation tests, and there was a significant correlation between cell-mediated immune response and bacteriological findings. There was a significant correlation between cell-mediated immune response and the level of serum antibodies on a group basis, but there was little correlation between the two systems on individual infected animals. Among vaccinated animals there was little or no correlation between cell-mediated immune and humoral responses. The whole-blood assay was found to be simple, fast, sensitive, and reproducible.

Primary immune response of guinea pig spleen cells in vitro

1978 ◽  
Vol 20 ◽  
pp. 131-141 ◽  
Author(s):  
J. Kettman ◽  
S. Ben-Sasson ◽  
J.U. Rudin
Keyword(s):  
Immune Response ◽  
Guinea Pig ◽  
Primary Immune Response ◽  
Spleen Cells

scholarly journals HETEROGENEITY OF THE CELLULAR IMMUNE RESPONSE

1971 ◽  
Vol 133 (2) ◽  
pp. 187-201 ◽  
Author(s):  
Robert C. Bast ◽  
Eleanor J. Manseau ◽  
Harold F. Dvorak
Keyword(s):  
Immune Response ◽  
Cellular Immune Response ◽  
Large Population ◽  
Specific Antigen ◽  
Lymphoid Cells ◽  
Lymphocyte Stimulation ◽  
Delayed Hypersensitivity ◽  
Skin Reactions ◽  
Cellular Immune

Lymph node cells from guinea pigs immunized with HSA in complete Freund's adjuvant were grown in cultures containing different concentrations of specific antigen. Stimulation of thymidine incorporation was induced with progressively lower concentrations of HSA at successive intervals after sensitization. Moreover, the intensity of delayed skin reactions and the magnitude of stimulation in vitro increased over the same interval. These events are considered compatible with an evolution of the cellular immune response resulting from the selection of lymphoid cells by decreasing concentrations of antigen in vivo. Cells from animals rendered tolerant to HSA failed to respond to specific antigen in culture. As tolerance waned, stimulation was achieved at high but not low antigen concentrations. Tolerance, measured by cutaneous reactivity or by lymphocyte stimulation, was less readily induced in animals sensitized with adjuvant containing a reduced concentration of mycobacteria. Lymph nodes from these animals contained a large population of cells reactive at high antigen concentration, presumably less susceptible to the toleragenic effect of intravenous antigen. The dissociation of delayed hypersensitivity and antibody formation observed early in the immune response and upon recovery from tolerance has permitted correlation of lymphocyte stimulation with delayed hypersensitivity and cutaneous basophil hypersensitivity respectively.

scholarly journals Immune response of Lewis rats to peptide C1 (residues 68-88) of guinea pig and rat myelin basic proteins.

1977 ◽  
Vol 146 (5) ◽  
pp. 1323-1331 ◽  
Author(s):  
R F Kibler ◽  
R B Fritz ◽  
F Chou ◽  
Jen Chou C-H ◽  
N Y Peacocke ◽  
...  
Keyword(s):  
Immune Response ◽  
T Cells ◽  
Amino Acid ◽  
Guinea Pig ◽  
Proliferative Response ◽  
Single Amino Acid ◽  
Helper Function ◽  
Circulating Antibodies ◽  
Stimulation Of

Peptide C1 (residues 68-88) from GP and rat BP differ by a single amino acid interchange at residue 79. This residue is serine in GP C1 and threonine in rat C1. GP C1 was encephalitogenic in Le rats at doses as low as 15 ng. Rat C1 was encephalitogenic at doses of 1,500 ng or greater. LNC from rats challenged with 25 X 10(-4) micronmol of GP C1 and 250 X 10(-4) micronmol of rat C1 showed a proliferative response in vitro to both peptides, but in each instance the magnitude of the response was greater to the GP peptide. GP C1 also induced higher levels of circulating antibodies at 25 X 10(-4) micronmol, but the specificity of antibodies produced by the two peptides was the same. These results have been interpreted as indicating that the presence of serine at position 79 in GP C1 results in the stimulation of greater numbers of T cells involved in (a) the induction of EAE, (b) the in vitro proliferative response and (c) helper function in antibody production.

Immune response of guinea pig lymphoid cells in vitro

1980 ◽  
Vol 50 (1) ◽  
pp. 216-223 ◽  
Author(s):  
J. Kettman ◽  
S. Ben-Sasson
Keyword(s):  
Immune Response ◽  
Guinea Pig ◽  
Lymphoid Cells

scholarly journals Development of a Guinea Pig Immune Response-Related Microarray and Its Use To Define the Host Response following Mycobacterium bovis BCG Vaccination

2006 ◽  
Vol 74 (2) ◽  
pp. 1436-1441 ◽  
Author(s):  
Julia A. Tree ◽  
Michael J. Elmore ◽  
Sajid Javed ◽  
Ann Williams ◽  
Philip D. Marsh
Keyword(s):  
Gene Expression ◽  
Immune Response ◽  
Immune Responses ◽  
Guinea Pig ◽  
Mycobacterium Bovis ◽  
Host Response ◽  
Custom Made ◽  
Guinea Pig Model ◽  
Type Response

ABSTRACT Immune responses in the guinea pig model are understudied because of a lack of commercial reagents. We have developed a custom-made guinea pig oligonucleotide microarray (81 spots) and have examined the gene expression profile of splenocytes restimulated in vitro from Mycobacterium bovis BCG-vaccinated and naive animals. Eleven genes were significantly (P < 0.05) up-regulated following vaccination, indicating a Th1-type response. These results show that microarrays can be used to more fully define immune profiles of guinea pigs.

Inhibition of Human and Animal Platelet Adhesiveness to Glass Bead Columns by Adenosine, Dipyridamole, Chlorpromazine and Acetylsalicylic Acid

1976 ◽  
Vol 36 (02) ◽  
pp. 401-410 ◽  
Author(s):  
Buichi Fujttani ◽  
Toshimichi Tsuboi ◽  
Kazuko Takeno ◽  
Kouichi Yoshida ◽  
Masanao Shimizu
Keyword(s):  
Guinea Pig ◽  
Acetylsalicylic Acid ◽  
Guinea Pigs ◽  
Glass Bead ◽  
Animal Species ◽  
Inhibitory Effect ◽  
Rabbit Platelet ◽  
Platelet Adhesiveness

SummaryThe differences among human, rabbit and guinea-pig platelet adhesiveness as for inhibitions by adenosine, dipyridamole, chlorpromazine and acetylsalicylic acid are described, and the influence of measurement conditions on platelet adhesiveness is also reported. Platelet adhesiveness of human and animal species decreased with an increase of heparin concentrations and an increase of flow rate of blood passing through a glass bead column. Human and rabbit platelet adhesiveness was inhibited in vitro by adenosine, dipyridamole and chlorpromazine, but not by acetylsalicylic acid. On the other hand, guinea-pig platelet adhesiveness was inhibited by the four drugs including acetylsalicylic acid. In in vivo study, adenosine, dipyridamole and chlorpromazine inhibited platelet adhesiveness in rabbits and guinea-pigs. Acetylsalicylic acid showed the inhibitory effect in guinea-pigs, but not in rabbits.

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