Brain Monoamines in Guinea Pigs with Experimental Allergic Encephalitis

1973 ◽  
Vol 45 (3) ◽  
pp. 341-351 ◽  
Author(s):  
E. Lycke ◽  
B.-E. Roos
Keyword(s):  
Guinea Pigs ◽  
Brain Monoamines ◽  
Experimental Allergic Encephalitis ◽  
Experimental Allergic

scholarly journals EXPERIMENTAL ALLERGIC ENCEPHALITIS

1972 ◽  
Vol 136 (1) ◽  
pp. 156-174 ◽  
Author(s):  
Lynn E. Spitler ◽  
Christine M. von Muller ◽  
H. Hugh Fudenberg ◽  
Edwin H. Eylar
Keyword(s):  
Amino Acid ◽  
Amino Acid Sequence ◽  
Cellular Immunity ◽  
Guinea Pigs ◽  
Native Protein ◽  
Brain Protein ◽  
Experimental Allergic Encephalitis ◽  
Experimental Allergic

The encephalitogenic determinant of brain protein, a nonapeptide having the amino acid sequence Phe-Ser-Trp-Gly-Ala-Glu-Gly-Gln-Lys, has been characterized and synthesized. In a previous study, analogues of this encephalitogenic peptide were synthesized and some were shown to be encephalitogenic while others were not. Guinea pigs were immunized with encephalitogenic peptides having amino acid sequences different from that in the native protein. These guinea pigs did not show cellular immunity in vivo (skin reactivity) or in vitro (lymphocyte stimulation or macrophage migration inhibition) to the encephalitogenic brain protein (EP) although they did show cellular immunity to the immunizing antigenic peptide. Guinea pigs immunized with an encephalitogenic peptide having the same amino acid sequence as the brain protein, or with a nonencephalitogenic peptide having the same amino acid sequence as the native protein but lacking the terminal lysine, did develop cellular immunity to the EP. Animals immunized with EP showed cellular immunity to this protein, but not to the encephalitogenic peptides. Animals immunized with nonencephalitogenic protein (NEP), prepared by altering the tryptophan residue of EP, did not develop disease but did show cellular immunity in vitro and in vivo to the EP. Animals protected from disease by immunization with NEP similarly showed cellular immunity to EP. Thus, the results suggest a dissociation between cellular immunity to EP and the production of experimental allergic encephalitis (EAE). Animals immunized with the encephalitogenic peptides develop EAE, but do not show cellular immunity to EP, and animals immunized with NEP show cellular immunity to EP but do not develop EAE. A fresh approach to the examination of the pathogenesis of EAE is now possible through the use of these well-characterized antigens.

Oligoclonal IgG in the cerebrospinal fluid of guinea pigs with acute experimental allergic encephalomyelitis

1982 ◽  
Vol 53 (3) ◽  
pp. 433-441 ◽  
Author(s):  
Abdolmohammad Rostami ◽  
Robert P. Lisak ◽  
Nadine Blanchard ◽  
Felicula Guerrero ◽  
Burton Zweiman ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
Experimental Allergic Encephalomyelitis ◽  
Guinea Pigs ◽  
Allergic Encephalomyelitis ◽  
Oligoclonal Igg ◽  
Experimental Allergic

Peptide length requirement for experimental allergic encephalomyelitis in guinea pigs

1980 ◽  
Vol 5 (1) ◽  
pp. 37-42 ◽  
Author(s):  
Shimon Levit ◽  
James M. Powers ◽  
Debra Milek ◽  
Steven W. Brostoff
Keyword(s):  
Experimental Allergic Encephalomyelitis ◽  
Guinea Pigs ◽  
Allergic Encephalomyelitis ◽  
Peptide Length ◽  
Experimental Allergic
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