Corneal Re-Epithelialization following Phototherapeutic Keratectomy for Recurrent Corneal Erosion as in vivo Model of Epithelial Wound Healing

2009 ◽  
Vol 223 (6) ◽  
pp. 414-418 ◽  
Author(s):  
Martin Baumeister ◽  
Jens Bühren ◽  
Christian Ohrloff ◽  
Thomas Kohnen
Keyword(s):  
Wound Healing ◽  
In Vivo Model ◽  
Phototherapeutic Keratectomy ◽  
Corneal Erosion ◽  
Epithelial Wound Healing ◽  
Recurrent Corneal Erosion

scholarly journals Human interleukin-4–treated regulatory macrophages promote epithelial wound healing and reduce colitis in a mouse model

2020 ◽  
Vol 6 (23) ◽  
pp. eaba4376 ◽  
Author(s):  
Timothy S. Jayme ◽  
Gabriella Leung ◽  
Arthur Wang ◽  
Matthew L. Workentine ◽  
Sruthi Rajeev ◽  
...  
Keyword(s):  
Wound Healing ◽  
Healthy Volunteers ◽  
Wound Repair ◽  
Interleukin 4 ◽  
Cellular Immunotherapy ◽  
Blood Monocytes ◽  
Systemic Delivery ◽  
Epithelial Wound Healing

Murine alternatively activated macrophages can exert anti-inflammatory effects. We sought to determine if IL-4–treated human macrophages [i.e., hM(IL4)] would promote epithelial wound repair and can serve as a cell transfer treatment for inflammatory bowel disease (IBD). Blood monocytes from healthy volunteers and patients with active and inactive IBD were converted to hM(IL4)s. IL-4 treatment of blood-derived macrophages from healthy volunteers and patients with inactive IBD resulted in a characteristic CD206+CCL18+CD14low/− phenotype (RNA-seq revealed IL-4 affected expression of 996 genes). Conditioned media from freshly generated or cryopreserved hM(IL4)s promoted epithelial wound healing in part by TGF, and reduced cytokine-driven loss of epithelial barrier function in vitro. Systemic delivery of hM(IL4) to dinitrobenzene sulphonic acid (DNBS)–treated Rag1−/− mice significantly reduced disease. These findings from in vitro and in vivo analyses provide proof-of-concept support for the development of autologous M(IL4) transfer as a cellular immunotherapy for IBD.

scholarly journals Immunohistological changes in skin wounds during the early periods of healing in a rat model

2012 ◽  
Vol 57 (No. 2) ◽  
pp. 77-82 ◽  
Author(s):  
F. Sabol ◽  
L. Dancakova ◽  
P. Gal ◽  
T. Vasilenko ◽  
M. Novotny ◽  
...  
Keyword(s):  
Wound Healing ◽  
Wide Spectrum ◽  
Smooth Muscle Actin ◽  
Healing Process ◽  
Skin Wound ◽  
Hair Follicles ◽  
In Vivo Model ◽  
Wound Healing Process ◽  
Rat Skin

The complexity of the wound healing process, which is still poorly understood, prompted us to perform an immunohistochemical investigation using rat skin as an in vivo model. Fifteen Sprague-Dawley rats were included in the experiment. Two round full thickness wounds, 4 mm in diameter, were made on the backs of all rats. Haematoxylin and eosin basic staining as well as antibodies against wide spectrum keratin, keratin 10, keratin 14, α-smooth muscle actin, vimentin, fibronectin, collagens Type 1 and 3, and the transcription factor Sox-2 were applied to paraffin and frozen sections of skin wound specimens two, six and fourteen days after surgery, respectively. New hair follicles with Sox-2-positive cells were present after fourteen days; keratin/vimentin positivity was restricted to specimens of day two. Collagen-3 expression prevailed over collagen-1 expression at all evaluated time intervals, except in the uninjured part of the dermis. In conclusion, rat skin wound healing is a dynamic process which can serve as a model for studying phenomena such as cell-cell interactions and transitions in vivo.

Full-Thickness Human Foreskin Transplantation onto Nude Rats as an In Vivo Model of Acute Human Wound Healing

2003 ◽  
Vol 111 (6) ◽  
pp. 1988-1997 ◽  
Author(s):  
Peter B. Petratos ◽  
Jie Chen ◽  
Robert A. Soslow ◽  
Clifford B. Bleustein ◽  
Diane Felsen ◽  
...  
Keyword(s):  
Wound Healing ◽  
In Vivo Model ◽  
Full Thickness ◽  
Nude Rats

Fibronectin Enhancement of Corneal Epithelial Wound Healing of Rabbits In Vivo

1984 ◽  
Vol 102 (3) ◽  
pp. 455-456 ◽  
Author(s):  
T. Nishida ◽  
S. Nakagawa ◽  
C. Nishibayashi ◽  
H. Tanaka ◽  
R. Manabe
Keyword(s):  
Wound Healing ◽  
Corneal Epithelial ◽  
Epithelial Wound Healing

scholarly journals An In Vivo Model of Wound Healing in Genetically Modified Skin-Humanized Mice

2004 ◽  
Vol 123 (6) ◽  
pp. 1182-1191 ◽  
Author(s):  
María José Escámez ◽  
Marta García ◽  
Fernando Larcher ◽  
Alvaro Meana ◽  
Evangelina Muñoz ◽  
...  
Keyword(s):  
Wound Healing ◽  
Genetically Modified ◽  
Humanized Mice ◽  
In Vivo Model

Effect of Ofloxacin on Corneal Epithelial Wound Healing Evaluated by In Vitro and In Vivo Methods

1993 ◽  
Vol 6 (2) ◽  
pp. 96-103 ◽  
Author(s):  
Steven S. Matsumoto ◽  
Michael E. Stern ◽  
Roger M. Oda ◽  
Corine R. Ghosn ◽  
Josephine W. Cheng ◽  
...  
Keyword(s):  
Wound Healing ◽  
Corneal Epithelial ◽  
Epithelial Wound Healing
Sign in / Sign up

Export Citation Format

Share Document