Minimal Molecular Size of Dextran Required to Elicit Heterologous Passive Cutaneous Anaphylaxis in Guinea Pigs

1972 ◽  
Vol 43 (2) ◽  
pp. 252-268 ◽  
Author(s):  
W. Richter
Keyword(s):  
Guinea Pigs ◽  
Molecular Size ◽  
Passive Cutaneous Anaphylaxis

PASSIVE CUTANEOUS ANAPHYLAXIS INDUCED IN GUINEA PIGS BY INSULINS AND THEIR COMPONENT CHAINS

1966 ◽  
Vol 44 (7) ◽  
pp. 989-996 ◽  
Author(s):  
S. Wilson ◽  
Marie A. Aprile ◽  
Louise Sasaki
Keyword(s):  
Guinea Pigs ◽  
Competitive Inhibition ◽  
The Other ◽  
Passive Cutaneous Anaphylaxis ◽  
Antigenic Determinants ◽  
Enzymic Digestion ◽  
Heterologous Antiserum ◽  
Wide Range ◽  
Homologous Antiserum

Small amounts of purified ox insulin and its component chains provoked passive cutaneous anaphylaxis (PCA) in guinea pigs previously injected intradermally with antiserum to ox insulin. Cod insulin and its chains also yielded a PCA reaction with antiserum to cod insulin, indicating that antibody-combining sites are located on both the A- and B-chains of insulin Enzymic digestion of the chain preparations essentially destroyed their ability to combine with anaphylactic antibodies, although the B-chain digests had some residual PCA reactivity.Ox and cod insulins gave positive PCA reactions with the heterologous antiserum but at higher levels than with the homologous antiserum, and none of the four chain preparations reacted with the heterologous antiserum. These results demonstrated differences between the antibody-combining sites on the chains of ox and cod insulins.Insulins from a wide range of species also yielded a PCA reaction with antisera to cod and ox insulins, suggesting that the different hormone preparations have certain antigenic determinants in common. Immunologic relationships between several insulins were derived from the competitive inhibition of one antiserum by the other when both were present in the same animal.

scholarly journals CONCOMITANT ANAPHYLACTIC SENSITIZATION AND CONTACT UNRESPONSIVENESS FOLLOWING THE INFUSION OR FEEDING OF PICRYL CHLORIDE TO GUINEA PIGS

1966 ◽  
Vol 124 (1) ◽  
pp. 69-80 ◽  
Author(s):  
Jerome R. Pomeranz ◽  
Philip S. Norman
Keyword(s):  
Large Dose ◽  
Guinea Pigs ◽  
Contact Hypersensitivity ◽  
Passive Cutaneous Anaphylaxis ◽  
Picryl Chloride ◽  
Circulating Antibody

Guinea pigs receiving one large dose of picryl chloride by the intravenous or oral routes commonly develop circulating antibody demonstrable by passive cutaneous anaphylaxis or by active anaphylaxis. They often concommittantly become unresponsive to the induction of delayed contact hypersensitivity by intracutaneous injections. Erythrocytes obtained from guinea pigs after infusion or feeding of picryl chloride may be used to sensitize other animals when injected with adjuvant. It is concluded that guinea pigs may be anaphylactically sensitized to simple chemicals by the intravenous and oral routes if a sufficient dose is administered.

scholarly journals RABBIT ANAPHYLACTIC ANTIBODY

1966 ◽  
Vol 123 (5) ◽  
pp. 935-950 ◽  
Author(s):  
Nathan J. Zvaifler ◽  
Elmer L. Becker
Keyword(s):  
Guinea Pig ◽  
Latent Period ◽  
Electrophoretic Mobility ◽  
Guinea Pigs ◽  
Hemagglutinating Activity ◽  
Antigenic Stimulation ◽  
Passive Cutaneous Anaphylaxis ◽  
Peak Activity ◽  
Rabbit Skin ◽  
Almost All

Antibody capable of sensitizing rabbit skin for passive cutaneous anaphylaxis is produced in the rabbit as early as 6 to 7 days following antigenic stimulation. It reaches peak activity around the 9th day and is gone by the 3rd wk. The antibody is heat labile, sensitive to treatment with mercaptoethanol, non-precipitating and does not fix complement. In order to demonstrate PCA activity a latent period is required of from 48 to 72 hr after introduction of the antibody into the rabbit's skin; the activity can persist for at least 17 days. It has a faster electrophoretic mobility than rabbit γG-globulin, and is eluted somewhat earlier than γG-globulin from Sephadex G-200, although distinctly after γM-globulin. No relationship was demonstrated between the rabbit PCA activity and the hemagglutinating activity found in the same sera. The rabbit anaphylactic antibody differs in almost all properties studies from the rabbit 7S antibody capable of sensitizing guinea pigs for PCA which arises at the same time. This latter antibody found early in immunization had properties which were indistinguishable from those described for the rabbit 7S antibody giving PCA in the guinea pig found in late hyperimmune sera.

scholarly journals SPECIFICITY OF FIXATION LOCI FOR HOMOCYTOTROPIC ANTIBODIES

1971 ◽  
Vol 134 (2) ◽  
pp. 381-394 ◽  
Author(s):  
Jack R. Battisto ◽  
Daniel Budman ◽  
Robert Freedman
Keyword(s):  
Guinea Pigs ◽  
Passive Cutaneous Anaphylaxis ◽  
Heat Stable

The heat-stable, homocytotropic antibody synthesized by guinea pigs to highly purified dextran has been studied by passive cutaneous anaphylaxis (PCA). Antibody arising in single guinea pigs was found to give PCA in some homologous recipients but not in others. Data are presented which indicate that the antibodies are bound in the skin of some animals to a greater degree than in that of others. This suggests some type of variability, perhaps allotypic, in the Fc portion of these immunoglobulins which relates to specificities of the loci where Fc becomes fixed in skin.

scholarly journals ELECTROPHORETIC SEPARATION AND PROPERTIES OF MOUSE ANTIHAPTEN ANTIBODIES INVOLVED IN PASSIVE CUTANEOUS ANAPHYLAXIS AND PASSIVE HEMOLYSIS

1964 ◽  
Vol 120 (2) ◽  
pp. 315-328 ◽  
Author(s):  
Ruth Sonntag Nussenzweig ◽  
Carmen Merryman ◽  
Baruj Benacerraf
Keyword(s):  
Guinea Pig ◽  
Guinea Pigs ◽  
Lytic Activity ◽  
Passive Cutaneous Anaphylaxis ◽  
Electrophoretic Separation ◽  
Sheep Erythrocytes ◽  
Electrophoretic Mobilities ◽  
Different Types ◽  
Specific Rabbit

Some properties of mouse antibodies produced by hyperimmunization with conjugated haptens, emulsified in complete adjuvant, have been investigated. Under these conditions two antigenically different types of precipitating antibody, with small differences in their electrophoretic mobilities, both migrating in the γ-region, are produced. Both antibody types were shown to be different from the ß2A-globulins) as revealed by the absence of reaction with specific rabbit antisera against mouse ß2A-myeloma globulins. γ2 or the slower migrating antibodies, similarly to what has been described for the guinea pig, were able to provoke lysis of antigen-coated tanned sheep erythrocytes, in the presence of complement. This activity could not be detected in the faster migrating antibody type. The slower migrating fractions of the γ2-antibodies were able to transfer passive cutaneous anaphylaxis (PCA) in the guinea pig, but not in their own species. γ1-Antibodies were found to be capable of sensitizing the mouse for cutaneous anaphylaxis. The sensitivity of these antibodies to reduction and alkylation was different, the lytic activity being practically abolished, the PCA titers in mice much reduced, and the PCA activity in guinea pigs only partially inactivated.

Polymerized Hemoglobin With Increased Molecular Size Reduces Toxicity in Healthy Guinea Pigs

2020 ◽  
Vol 3 (5) ◽  
pp. 2976-2985 ◽  
Author(s):  
Alexander T. Williams ◽  
Cynthia R. Muller ◽  
Allyn M. Eaker ◽  
Donald A. Belcher ◽  
Crystal Bolden-Rush ◽  
...  
Keyword(s):  
Guinea Pigs ◽  
Molecular Size ◽  
Polymerized Hemoglobin

ELISA for Guinea-pig IgG1: An Alternative to the PCA Test for Relative Allergenicity

1991 ◽  
Vol 19 (1) ◽  
pp. 8-14
Author(s):  
Annette Prentø
Keyword(s):  
Guinea Pig ◽  
Guinea Pigs ◽  
Passive Cutaneous Anaphylaxis ◽  
Relative Importance ◽  
Elisa Method ◽  
Passive Cutaneous Anaphylaxis Test

An ELISA method to replace the PCA (passive cutaneous anaphylaxis) test has been developed for use in the evaluation of relative allergenicity in guinea-pigs (Dunkin-Hartley). The method detects antigen-specific IgG1 antibodies formed in the guinea-pig during sensitisation, and correlates well with the corresponding PCA test. The relative importance of IgG1 and IgE for relative allergenicity in the Dunkin-Hartley guinea-pig strain is discussed.

Sign in / Sign up

Export Citation Format

Share Document