In vivo and in vitro Immunological Responses to Heterologous and Homologous Thyroglobulin in Guinea Pigs Immunized with Heterologous Thyroid Extracts in Freund’s Complete Adjuvant

1971 ◽  
Vol 41 (1) ◽  
pp. 138-139
Author(s):  
M. Ricci ◽  
S. Romagnani ◽  
A. Passaleva ◽  
G. Biliotti
Keyword(s):  
Guinea Pigs ◽  
Freund’S Complete Adjuvant ◽  
Immunological Responses ◽  
Freund's Complete Adjuvant

scholarly journals Evaluation of different immunization protocols with P. brasiliensis antigens in Guinea pigs

1992 ◽  
Vol 34 (3) ◽  
pp. 243-249
Author(s):  
Amélia Kamegasawa ◽  
Maria Tereza Rezkallah Iwasso ◽  
Rosa Viero ◽  
Marcello Franco
Keyword(s):  
Immune Response ◽  
Guinea Pigs ◽  
Protective Immunity ◽  
Migration Inhibition ◽  
Freund’S Complete Adjuvant ◽  
Future Studies ◽  
Freund's Complete Adjuvant ◽  
Particulate Antigen ◽  
Long Time ◽  
Macrophage Migration Inhibition

The objective of the present study was to develop an efficient and reproducible protocol of immunization of guinea pigs with P. brasiliensis antigens as an animal model for future studies of protective immunity mechanisms. We tested three different antigens (particulate, soluble and combined) and six protocols in the presence and absence of Freund's complete adjuvant and with different numbers of immunizing doses and variable lenght of time between the last immunizing dose and challenge. The efficacy of the immunizing protocol was evaluated by measuring the humoral and cellular anti-P. brasiliensis immune response of the animals, using immuno-diffusion, skin test and macrophage migration inhibition test. It was observed that: 1. Three immunizing doses of the antigens induced a more marked response than two doses; 2. The highest immune response was obtained with the use of Freund's complete adjuvant; 3. Animals challenged a long time (week 6) after the last immunizing dose showed good anti-P. brasiliensis immune response; 4. The particulate antigen induced the lowest immune response. The soluble and the combined antigens were equally efficient in raising good humoral and cellular anti-P. brasiliensis immune response

scholarly journals Enzymatic changes in various organs of guinea pigs caused by the administration of Freund's complete adjuvant.

1983 ◽  
Vol 6 (12) ◽  
pp. 974-982 ◽  
Author(s):  
TAKAAKI AOYAGI ◽  
TAKAO WADA ◽  
TAKAAKI NISHIKIORI ◽  
FUKIKO KOJIMA ◽  
MACHIKO NAGAI ◽  
...  
Keyword(s):  
Guinea Pigs ◽  
Freund’S Complete Adjuvant ◽  
Freund's Complete Adjuvant ◽  
Enzymatic Changes

Airway Epithelial Cells in Various Models of Bronchial Allergic Inflammation in Guinea Pigs

1992 ◽  
Vol 5 (3) ◽  
pp. 165-172
Author(s):  
J.P. Tarayre ◽  
M. Aliaga ◽  
M. Barbara ◽  
N. Malfetes ◽  
S. Vieu
Keyword(s):  
Epithelial Cells ◽  
Anaphylactic Reaction ◽  
Guinea Pigs ◽  
Allergic Inflammation ◽  
Lavage Fluid ◽  
Airway Epithelial Cells ◽  
Airway Epithelial ◽  
Freund’S Complete Adjuvant ◽  
Freund's Complete Adjuvant ◽  
Similar Intensity

The number of airway epithelial cells (AEC) in bronchus lumen after various types of anaphylactic shock of similar intensity was determined in guinea pigs. Aerosol-induced anaphylactic shocks were studied after four types of active sensitization: sensitization by IM injection of 30 mg/kg ovalbumin mixed with Freund's complete adjuvant; sensitization by IM injection of 30 mg/kg ovalbumin; sensitization by IP injection of 1 μg ovalbumin and 50 mg A1 (OH)3 by animal; sensitization by 2 exposures to 1%-ovalbumin aerosol at a 7-day interval. One, 3,6,24 and 48 h after the anaphylactic reaction the number of AEC in bronchoalveolar lavage fluid (BALF) was counted in comparison to guinea pigs sensitized but exposed to 0.9% NaCl aerosol. The number of AEC was significantly increased only 24–48 h after the shock in animals sensitized with ovalbumin in Freund's complete adjuvant. In animals passively sensitized by antiserum obtained with sensitization by ovalbumin in Freund's complete adjuvant and challenged by IV or aerosol administration of antigen, non rise in the number of AEC was obtained 24–48 h after the shock. The higher amount of AEC 24–48 h after the anaphylactic reaction in animals actively sensitized by ovalbumin in Freund's complete adjuvant could be related to the higher increase in the number of neutrophils and of mononuclears obtained in the BALF at these times in comparison to other types of active sensitization. The rise in the number of AEC only after the active reaction seems to show the part played by T-lymphocytes in the induction of this effect. The increase in the number of eosinophils in BALF and the appearance of the hyperreactivity cannot be related to the rise in the AEC in bronchus lumen.

Experimental Amyloidosis Induced by Immune Complex

1971 ◽  
Vol 29 ◽  
pp. 582-583
Author(s):  
A. Kawaoi
Keyword(s):  
Immune Complex ◽  
Systemic Amyloidosis ◽  
Human Diseases ◽  
Experimental Amyloidosis ◽  
Freund’S Complete Adjuvant ◽  
Freund's Complete Adjuvant ◽  
Immunological Approach ◽  
Experimentally Induced

Numbers of immunological approach have been made to the amyloidosis through the variety of predisposing human diseases and the experimentally induced animals by the greater number of agents. The results suggest an important role of impaired immunity involving both humoral and cell-mediated aspects.Recently the author has succeeded in producing amyloidosis in the rabbits and mice by the injections of immune complex of heat denatured DNA.The aim of this report is to demonstrate the details of the ultrastructure of the amyloidosis induced by heterologous insoluble immune complex. Eleven of twelve mice, dd strain, subcutaneously injected twice a week with Freund's complete adjuvant and four of seven animals intraperitonially injected developed systemic amyloidosis two months later from the initial injections. The spleens were electron microscopically observed.

Involvement of 5-Hydroxytryptamine in Platelet Aggregation In Vivo in Rats and Guinea Pigs

1989 ◽  
Vol 61 (03) ◽  
pp. 463-467 ◽  
Author(s):  
G M Smith
Keyword(s):  
Platelet Count ◽  
Guinea Pigs ◽  
Platelet Adhesion ◽  
Adenosine Diphosphate ◽  
Rate Of Return ◽  
Low Doses ◽  
Dose Dependent ◽  
Higher Doses ◽  
Rat Platelets

SummaryIn this study, 5-hydroxytryptamine (5-HT) caused a dose- dependent fall in the circulating platelet count suggesting that 5-HT receptors are activated in rat platelets to cause platelet adhesion and aggregation. When low doses of adenosine diphosphate (ADP) were simultaneously injected with 5-HT, there was a significant potentiation of the responses to ADR Ketanserin significantly reduced the potentiated responses. When higher doses of ADP were infused with bolus injections of 5-HT there was no potentiation and ketanserin did not reduce these responses. Ketanserin did not inhibit the collagen-induced fall in circulating platelet count, but did significantly increase the rate of return to the basal platelet count compared with control. 5-HT did not cause a fall in platelet count in guinea-pigs

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