Copy Number Variation of Testis-Specific Protein, Y-Encoded (TSPY) in 14 Different Breeds of Cattle (Bos taurus)

2009 ◽  
Vol 3 (4) ◽  
pp. 205-213 ◽  
Author(s):  
C.K. Hamilton ◽  
L.A. Favetta ◽  
G.P. Di Meo ◽  
S. Floriot ◽  
A. Perucatti ◽  
...  
Keyword(s):  
Copy Number Variation ◽  
Copy Number ◽  
Bos Taurus ◽  
Specific Protein ◽  
Number Variation

scholarly journals Copy number variation of testis‐specific protein Y‐encoded (TSPY) among different breeds of cattle (Bos taurus) and related individuals

2009 ◽  
Vol 23 (S1) ◽  
Author(s):  
Christine Karin Hamilton ◽  
Laura Favetta ◽  
Giulia Pia Di Meo ◽  
Sandrine Floriot ◽  
Angela Perucatti ◽  
...  
Keyword(s):  
Copy Number Variation ◽  
Copy Number ◽  
Bos Taurus ◽  
Specific Protein ◽  
Number Variation ◽  
Related Individuals

175 Testis-Specific Protein Y Encoded Copy Number Variation in In Vitro-Produced Bovine Early Embryos

2018 ◽  
Vol 30 (1) ◽  
pp. 227
Author(s):  
N.Y. Rho ◽  
L. A. Favetta ◽  
T. Mogas ◽  
W. A. King
Keyword(s):  
Copy Number Variation ◽  
Copy Number ◽  
Specific Protein ◽  
Development Rate ◽  
Male Reproduction ◽  
Cell Stage ◽  
Early Embryos ◽  
Number Variation ◽  
Developmental Rates

Testis-specific protein Y (TSPY) is one of the genes located in the male-specific region of the Y chromosome involved in male reproduction, particularly in spermatogenesis. It has been shown to have different copy number (CN) in different species, in different individuals within a species and in the same family (i.e. among brothers from the same father). Previous studies from our laboratory in the bovine species showed that different TSPY CN was correlated with fertility, as bulls with higher CN showed higher fertility rate. In this study, we investigated development rate in in vitro-produced (IVP) bovine early embryos produced by sex-sorted semen (X and Y) from 3 bulls (1, 2, and 3) and TSPY copy number variation (CNV) in the male blastocysts. The IVP embryos by the 3 different types of semen (sub-divided: 1X, 1Y, 2X, 2Y, 3X, 3Y) and parthenotes (PART) for the negative control were assessed for developmental rates (shown in Table 1 below) and individually collected at each cell stage from zygote to blastocyst to extract DNA for TSPY CN analysis. The DNA from individual blastocysts was treated with a restriction enzyme for relative quantification of TSPY CN using digital droplet PCR (ddPCR) with SRY, known to have only one copy in bovine, as a reference gene. Developmental data showed that Y-carrying semen had higher rates both in cleavage and blastocyst compared with X-carrying semen from the same bull. Particularly, bull 3 (both 3X and 3Y) had the highest developmental rates compared with the other 2 types of bulls. TSPY was detected in male blastocyst, where both parthenotes and female embryos were negative. Relative CN of TSPY versus SRY from blastocysts produced from bulls 1, 2, and 3 averaged to 87.5, 55, and 20, respectively. Interestingly, the CN among brother blastocysts showed a variation of less than 7.5, where bull-to-bull variation was approximately from 20 to 60 copies. Our results show that TSPY CN in the embryo varies according to the sire and among brother blastocysts. Moreover, TSPY CNV among brother blastocysts allows us to speculate that individually different recombination events occurred in meiosis during spermatogenesis. Table 1.Embryo development rate by semen type This research was funded by the Canada Research Chair’s program, Natural Sciences and Engineering Research Council of Canada and the Ontario Veterinary College.

scholarly journals Population differentiated copy number variation of Bos taurus, Bos indicus and their African hybrids

BMC Genomics ◽  
2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Jisung Jang ◽  
Kwondo Kim ◽  
Young Ho Lee ◽  
Heebal Kim
Keyword(s):  
Copy Number Variation ◽  
Copy Number ◽  
Bos Taurus ◽  
Bos Indicus ◽  
Olfactory Receptors ◽  
Read Depth ◽  
Pathogen Resistance ◽  
Individual Level ◽  
Copy Numbers ◽  
Number Variation

Abstract Background CNV comprises a large proportion in cattle genome and is associated with various traits. However, there were few population-scale comparison studies on cattle CNV. Results Here, autosome-wide CNVs were called by read depth of NGS alignment result and copy number variation regions (CNVRs) defined from 102 Eurasian taurine (EAT) of 14 breeds, 28 Asian indicine (ASI) of 6 breeds, 22 African taurine (AFT) of 2 breeds, and 184 African humped cattle (AFH) of 17 breeds. The copy number of every CNVRs were compared between populations and CNVRs with population differentiated copy numbers were sorted out using the pairwise statistics VST and Kruskal-Wallis test. Three hundred sixty-two of CNVRs were significantly differentiated in both statistics and 313 genes were located on the population differentiated CNVRs. Conclusion For some of these genes, the averages of copy numbers were also different between populations and these may be candidate genes under selection. These include olfactory receptors, pathogen-resistance, parasite-resistance, heat tolerance and productivity related genes. Furthermore, breed- and individual-level comparison was performed using the presence or copy number of the autosomal CNVRs. Our findings were based on identification of CNVs from short Illumina reads of 336 individuals and 39 breeds, which to our knowledge is the largest dataset for this type of analysis and revealed important CNVs that may play a role in cattle adaption to various environments.

scholarly journals Ectopic KIT Copy Number Variation Underlies Impaired Migration of Primordial Germ Cells Associated with Gonadal Hypoplasia in Cattle (Bos taurus)

PLoS ONE ◽  
2013 ◽  
Vol 8 (9) ◽  
pp. e75659 ◽  
Author(s):  
Heli Venhoranta ◽  
Hubert Pausch ◽  
Michal Wysocki ◽  
Izabela Szczerbal ◽  
Reetta Hänninen ◽  
...  
Keyword(s):  
Copy Number Variation ◽  
Germ Cells ◽  
Copy Number ◽  
Bos Taurus ◽  
Primordial Germ Cells ◽  
Number Variation

scholarly journals Copy number variation of ZNF280BY in Bos taurus and Bos indicus breeds

2019 ◽  
Vol 72 (04) ◽  
pp. 403-407
Author(s):  
JEEVAN C . ◽  
Indrajit Ganguly . ◽  
Siddharth Deswal . ◽  
Sanjeev Singh . ◽  
S P Dixit .
Keyword(s):  
Copy Number Variation ◽  
Copy Number ◽  
Bos Taurus ◽  
Bos Indicus ◽  
Number Variation

Identification of Novel Germline and Tumor-Specific Nucleotide Variants and Copy Number Variation in Clival Chordomas by Exome Sequencing

2015 ◽  
Vol 76 (S 01) ◽  
Author(s):  
Georgios Zenonos ◽  
Peter Howard ◽  
Maureen Lyons-Weiler ◽  
Wang Eric ◽  
William LaFambroise ◽  
...  
Keyword(s):  
Copy Number Variation ◽  
Exome Sequencing ◽  
Copy Number ◽  
Number Variation ◽  
Specific Nucleotide

The impact of paralog genes: detection of copy number variation in spinal muscle atrophy patients

BIOCELL ◽  
2018 ◽  
Vol 42 (3) ◽  
pp. 87-91 ◽  
Author(s):  
Sergio LAURITO ◽  
Juan A. CUETO ◽  
Jimena PEREZ ◽  
Mar韆 ROQU�
Keyword(s):  
Copy Number Variation ◽  
Muscle Atrophy ◽  
Copy Number ◽  
Number Variation ◽  
The Impact
Sign in / Sign up

Export Citation Format

Share Document