Nuclear Localisation of DNA Polymerase α and DNA Synthesis in Polyoma Virus Infected Mouse Cells

Oncology ◽  
1977 ◽  
Vol 34 (5) ◽  
pp. 190-192 ◽  
Author(s):  
U. Wintersberger ◽  
E. Wintersberger
Keyword(s):  
Dna Synthesis ◽  
Infected Mouse ◽  
Dna Polymerase ◽  
Polyoma Virus ◽  
Nuclear Localisation ◽  
Dna Polymerase Α ◽  
Mouse Cells

Structure of Polyoma Virus mRNAs Synthesized in Productively Infected Mouse Cells

1979 ◽  
Vol 13 (1) ◽  
pp. 45 ◽  
Author(s):  
R. KAMEN ◽  
J. FAVALORO ◽  
J. PARKER ◽  
R. TREISMAN ◽  
A.J. FLAVELL ◽  
...  
Keyword(s):  
Infected Mouse ◽  
Polyoma Virus ◽  
Mouse Cells ◽  
Virus Mrnas

scholarly journals The algorithm of estimation of the K m values for primers in DNA synthesis catalyzed by human DNA polymerase α

FEBS Letters ◽  
1996 ◽  
Vol 399 (1-2) ◽  
pp. 113-116 ◽  
Author(s):  
Tat'yana I. Kolocheva ◽  
Galiya A. Maksakova ◽  
Ol'ga D. Zakharova ◽  
Georgy A. Nevinsky
Keyword(s):  
Dna Synthesis ◽  
Dna Polymerase ◽  
Dna Polymerase Α ◽  
Human Dna

scholarly journals Poly(adenosine diphosphate ribose) polymerase activity and nicotinamide adenine dinucleotide in differentiating cardiac muscle

1976 ◽  
Vol 154 (2) ◽  
pp. 387-393 ◽  
Author(s):  
W C. Claycomb
Keyword(s):  
Cyclic Amp ◽  
Dna Synthesis ◽  
Cardiac Muscle ◽  
Dna Polymerase ◽  
Specific Activity ◽  
Polymerase Activity ◽  
Neonatal Rat ◽  
Dibutyryl Cyclic Amp ◽  
Fresh Tissue ◽  
Dna Polymerase Α

Poly(ADP-ribose) polymerase activity in nuclei isolated from differentiating cardiac muscle of the rat has been characterized and its activity measured during development. Optimum enzyme activity is observed at pH 8.5. Poly(ADP-ribose) polymerase is inhibited by ATP, thymidine, nicotinamide, theophylline, 3-isobutyl-1-methylxanthine and caffeine and stimulated by actinomycin D. The activity measured under optimal assay conditions increases during differentiation of cardiac muscle and is inversely related to the rate of DNA synthesis and to the activities of DNA polymerase α and thymidine kinase. When DNA synthesis and the activity of DNA polymerase α are inhibited in cardiac muscle of the 1-day-old neonatal rat by dibutyryl cyclic AMP or isoproterenol, the specific activity of poly(ADP-ribose) polymerase measured in isolated nuclei is increased. The concentration of NAD+ in cardiac muscle increases during postnatal development. In the adult compared with the 1-day-old neonatal rat the concentration of NAD+ relative to fresh tissue weight, DNA or protein increased 1.7-fold, 5.2-fold or 1.4-fold respectively. The concentration of NAD+ in cardiac muscle of the 1-day-old neonatal rat can be increased by approx. 20% by dibutyryl cyclic AMP. These data suggest that NAD+ and poly(ADP-ribose) polymerase may be involved with the repression of DNA synthesis and cell proliferation in differentiating cardiac muscle.

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